重组人凋亡素2配体对放射线诱导鼻咽癌CNE细胞凋亡作用的研究

发布时间：2018-06-04 08:14

本文选题：TNF相关凋亡诱导配体 + 放射疗法　；参考：《青岛大学》2014年硕士论文

【摘要】：目的本实验旨在研究重组人凋亡素2配体(Apo-2ligand, Apo-2L)与放射线协同对体外培养的鼻咽癌CNE细胞增殖周期及凋亡率的影响。方法MTT法检测不同浓度Apo-2L对鼻咽癌CNE细胞凋亡的影响,并确定Aop-2L与细胞作用时间。将细胞随机分为4组：对照组(CK组)、Apo-2L组(T组)、Apo-2L+放射组(T-R组)及单纯放射组(R组)；给予不同的组间处理后分别将4组细胞制成细胞悬液,将所得细胞悬液进行流式细胞仪分析测得各组细胞凋亡率和细胞周期变化。结果MTT法示鼻咽癌CNE细胞的抑制率与Apo-2L的浓度成正相关；当取Apo-2L浓度为100ng/ml作用CNE细胞24h,Apo-2L+放射组凋亡率较其它各组明显升高,P0.05,有统计学意义；Apo-2L+放射组G2/M期细胞比率显著降低,与其它各组相比有统计学差异,P0.05。结论Apo-2L对体外培养的鼻咽癌CNE细胞有抑制增殖和促进凋亡作用,其浓度与凋亡作用正相关；Apo-2L联合放射线可以明显提高CNE细胞的凋亡率,并促使细胞周期同步化分布。
[Abstract]:Objective to investigate the effects of recombinant human apoptin 2 ligand Apo-2 ligand (Apo-2L) and radiation on the proliferation cycle and apoptosis rate of nasopharyngeal carcinoma (NPC) CNE cells cultured in vitro. Methods MTT assay was used to detect the effect of different concentrations of Apo-2L on the apoptosis of nasopharyngeal carcinoma (NPC) CNE cells, and to determine the time of interaction between Aop-2L and the cells. The cells were randomly divided into four groups: control group (CK group), Apo-2L group (T group) and simple radiation group (group R). The apoptosis rate and cell cycle were measured by flow cytometry. Results MTT assay showed that the inhibitory rate of CNE cells in nasopharyngeal carcinoma was positively correlated with the concentration of Apo-2L, and that the apoptosis rate of CNE cells in 100ng/ml group was significantly higher than that in other groups (P 0.05), and the ratio of G 2 / M phase cells in Apo-2L radiation group was significantly lower than that in other groups. Compared with other groups, there was a significant difference (P 0.05). Conclusion Apo-2L can inhibit proliferation and promote apoptosis of CNE cells cultured in vitro. The concentration of Apo-2L in combination with radiation can significantly increase the apoptosis rate of CNE cells and promote the synchronous distribution of cell cycle.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.63

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