重组人内皮抑素对氧诱导视网膜新生血管抑制作用的研究

发布时间：2018-06-04 13:27

本文选题：视网膜新生血管 + 内皮抑素　；参考：《石河子大学》2013年硕士论文

【摘要】：目的：探讨重组人血管内皮抑素（恩度，ENDOSTAR）对高氧诱导的小鼠视网膜病变（oxygen-inducedretinopathy,OIR）模型中新生血管的抑制作用方法：将60只C57BL/6J小鼠随机分为正常对照组（A组），高氧对照组（B组），生理盐水对照组（C组），恩度干预组（D组），每组15只，除正常对照组(A组)外，均建立OIR模型。正常对照组(A组)，高氧对照组(B组)小鼠不做处理，生理盐水对照组(C组)于第12d给予腹腔注射0.1mlNS，恩度干预组(D组)于第12d给予腹腔注射rh.ES注射液0.1ml，连续注射5d，鼠龄17d时，视网膜组织切片行HE染色计数突破内界膜的新生血管内皮细胞核数目；应用免疫组织化学染色法检测视网膜中VEGF的表达。结果：HE染色正常对照组（A组）平均每张切片切片突破内界膜的内皮细胞细胞核个数为(0．97±0．04)个，高氧对照组（B组）平均每张切片突破内界膜的内皮细胞细胞核个数为(24.30±1.73)个。生理盐水对照组（C组）平均每张切片突破内界膜的内皮细胞细胞核个数为个数为(22.58±1.54)个。恩度干预组（D组）突破视网膜内界膜的血管内皮细胞核数目为(3.38±0.27)个。高氧对照组（B组）和生理盐水对照组（C组）突破内界膜的新生血管内皮细胞核数目明显多于正常对照组（A组），差异均有显著统计学意义(P＜0.01)；恩度干预组（D组）视网膜突破内界膜的新生血管内皮细胞核数目明显减少，与高氧对照组（B组）和生理盐水对照组（C组）比较，差异均有显著统计学意义(P＜0.01)；恩度干预组（D组）与正常对照组（A组）比较差异具有显著统计学意义(P0.05)。免疫组织化学检测显示，正常对照组（A组）:视网膜各层排列整齐，VEGF见弱阳性表达，主要位于神经纤维层和内核层；高氧对照组（B组）：视网膜组织结构紊乱，明显水肿，可见大量VEGF表达，呈棕黄色，以内界膜，神经纤维层及新生血管内皮细胞表达最明显。生理盐水对照组（C组）：视网膜各层组织水肿，细胞排列紊乱，VEGF分布在内界膜，神经纤维层及新生血管内皮细胞，，呈棕黄色，表达强；恩度干预组（D组）：VEGF表达不明显，各层均匀表达呈弱阳性。生理盐水对照组（C组）分别与正常对照组（A组）比较，差异均有显著性统计学意义(P0.01)；高氧对照组（B组），生理盐水对照组（C组）分别与恩度干预组（D组）比较，差异均有显著性统计学意义(P0.01)。恩度干预组（D组）与正常对照组（A组）比较，差异具有统计学意义(P0.05)。结论：腹腔内注射恩度可抑制小鼠模型中的视网膜新生血管形成，有望成为防治血管增生性视网膜病变的一种方法。
[Abstract]:Objective: To investigate the inhibitory effect of recombinant human endostatin (ENDOSTAR) on neovascularization in the oxygen-inducedretinopathy (OIR) model of hyperoxia induced mice
Methods: 60 C57BL/6J mice were randomly divided into normal control group (group A), hyperoxic control group (group B), normal saline control group (group C), and degree intervention group (group D), 15 rats in each group, except the normal control group (group A), the OIR model was established. The normal control group (A group), the hyperoxic control group (B group) mice did not do, the saline control group (C group) was in 12D. Intraperitoneal injection of 0.1mlNS, D group (Group) was given rh.ES injection 0.1ml by intraperitoneal injection at 12D, continuous injection of 5D, and rat age 17D, the number of neovascular endothelial nuclei breaking through the inner boundary membrane was counted by HE staining, and the expression of VEGF in the retina was detected by immunohistochemistry.
Results: the average number of nuclei of endothelial cells in the inner boundary membrane of each slice in the normal control group (group A) was (0.97 + 0.04), and the average number of nuclei of the endothelial cell nuclei of each slice breaking through the inner boundary membrane in the high oxygen control group (group B) was (24.30 + 1.73). The average of each slice in the saline control group (group C) broke through the inner boundary membrane. The number of cell nuclei of endothelial cells was (22.58 + 1.54). The number of nuclei of vascular endothelial cells that broke through the inner boundary membrane of the retina was (3.38 + 0.27). The number of nuclei in the neovascularization of the neovascularization in the hyperoxic control group (group B) and the saline control group (group C) was more than that of the normal control group (group A). Significant statistical significance (P < 0.01); the number of neovascular endothelial nuclei of the retinal breakthroughs in the retina was significantly reduced, compared with the hyperoxia control group (group B) and the saline control group (group C), the difference was statistically significant (P < 0.01). The difference between the degree intervention group (D group) and the normal control group (group A) was significantly different. Significant statistical significance (P0.05). The immunohistochemistry test showed that the normal control group (group A): the retina layers were arranged neatly, VEGF was weakly positive, mainly in the nerve fiber layer and the core layer, and the hyperoxia control group (group B): the retinal tissue structure was disorganized, the edema was obvious, and a large number of VEGF expressions were seen, with brown yellow, inner boundary membrane and nerve fiber. The expression of vascular endothelial cells in the vascular layer and neovascularization was most obvious. In the normal saline control group (group C), the tissues of the retina were edema, the cells were arranged in disorder, the VEGF distribution of the inner boundary membrane, the nerve fiber layer and the neovascular endothelial cells were brown and strong, and the expression of:VEGF was not obvious in the degree of grace intervention group (group D), and the homogeneous expression in each layer was weak positive. Compared with the normal control group (group A), the saline control group (group C) had significant statistical significance (P0.01), and in the hyperoxic control group (group B), the normal saline control group (group C) compared with the ENN intervention group (D group), the difference was statistically significant (P0.01). The difference was compared with the normal control group (D group) and the normal control group (A group). Statistical significance (P0.05).
Conclusion: intraperitoneal injection of Endostar inhibits the formation of retinal neovascularization in mouse models, and is expected to become a method for prevention and treatment of vascular proliferative retinopathy.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R774.1

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