早期鼻咽癌基因甲基化谱的初步构建

发布时间：2018-06-05 06:35

本文选题：早期鼻咽癌 + 基因芯片　；参考：《中山大学学报(医学科学版)》2014年06期

【摘要】：【目的】分析早期鼻咽癌的基因甲基化状态,初步构建早期鼻咽癌的基因甲基化谱。【方法】收集I期(T1N0M0)鼻咽癌石蜡标本及炎性增生性鼻咽组织各4例,分别提取DNA,富集两组样品基因组甲基化片段后采用甲基化芯片(Nimble Gen)对两组标本进行高通量测序分析,对杂交信号进行扫描及初步数据处理,分析并筛选出两组标本的差异甲基化基因。【结果】4例鼻咽癌石蜡标本中均出现甲基化的基因数目为1 134个,而4例炎性增生性鼻咽组织中均出现甲基化的基因有2 091个,4例鼻咽癌标本全部发生甲基化但4例炎性增生性鼻咽组织全未发生甲基化的基因有218个,提示此218个基因可能为早期鼻咽癌患者的甲基化基因谱。另外,4例鼻咽癌标本全未发生甲基化而4例炎性增生性鼻咽组织全部发生甲基化的基因数目为1 558个。【结论】本研究初步构建了早期鼻咽癌的基因甲基化谱,为进一步建立更为精确的甲基化谱奠定了基础,同时也为寻找鼻咽癌新的分子标志物提供了可能。
[Abstract]:[Objective] to analyze the methylation status of early nasopharyngeal carcinoma gene and construct the gene methylation spectrum of early nasopharyngeal carcinoma. [Methods] 4 cases of I (T1N0M0) nasopharyngeal carcinoma and 4 cases of inflammatory hyperplastic nasopharynx were collected, and DNA were extracted respectively, and the methylation chips (Nimble Gen) were used in the two groups after the enrichment of two groups of genomic methylation fragments. High flux sequencing analysis, scanning and preliminary data processing of hybrid signals, analysis and screening of two groups of differential methylation genes. [results] 4 cases of nasopharyngeal carcinoma paraffin specimens were 1134 of the number of methylated genes, and 4 cases of inflammatory hyperplastic nasopharyngeal tissues were methylated in 2091 genes. 4 cases of nasopharyngeal carcinoma were all methylated but 218 genes were not methylated in 4 cases of inflammatory hyperplastic nasopharynx, which suggested that the 218 genes might be the methylation gene spectrum of early nasopharyngeal carcinoma. In addition, 4 cases of nasopharyngeal carcinoma were methylation and 4 cases of inflammatory hypertrophic nasopharyngeal tissue were all methylation genes. The number is 1558. [Conclusion] this study has preliminarily constructed the gene methylation spectrum of early nasopharyngeal carcinoma, which lays the foundation for the further establishment of more accurate methylation spectrum, and also provides a possibility for finding new molecular markers of nasopharyngeal carcinoma.
【作者单位】：南方医科大学研究生学院;广东省人民医院∥广东省医学科学院耳鼻咽喉头颈外科;
【基金】：广州市科技计划项目(2011J4300067)
【分类号】：R739.63

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