Periostin过表达对鼻咽癌6-10B细胞侵袭和迁移的影响及机制

发布时间：2018-06-06 05:02

本文选题：Periostin + 鼻咽癌　；参考：《解剖学报》2014年04期

【摘要】：目的探讨外源表达periostin蛋白对鼻咽癌(NPC)6-10B细胞侵袭和迁移能力的影响及机制。方法采用脂质体转染技术建立稳定高表达periostin的6-10B细胞,RT-PCR结合Western blotting检测转染效率,Tanswell分析periostin高表达后6-10B细胞侵袭和迁移能力的改变,明胶酶谱法检测基质金属蛋白酶(MMPs)活性的变化;免疫组织化学检测整合素(integrin)αvβ5在转染periostin前后6-10B细胞中的表达以及其与periostin在鼻咽癌和正常鼻咽黏膜组织中的表达,image-pro Plus 6.0软件进行图像分析及吸光度测定,统计学分析鼻咽癌组织中periostin和integrinαvβ5表达强度的相关性。结果 Periostin过表达的6-10B细胞侵袭、迁移能力明显增强,细胞培养上清中MMP-2、MMP-9活性增加,integrinαvβ5在periostin过表达的6-10B细胞及NPC组织的表达明显强于对照组,Spearman相关性分析显示,integrinαvβ5和periostin在NPC组织中的表达强度成正相关(r=0.682,P=0.000)。结论外源表达periostin能促进NPC的侵袭和转移,其机制可能是通过与NPC细胞膜上integrinαvβ5受体结合而提高MMPs的活性。
[Abstract]:Objective to investigate the effect and mechanism of exogenous expression of periostin protein on invasion and migration of nasopharyngeal carcinoma (NPC) NPC-610B cells. Methods Liposome transfection technique was used to establish stable and high expression periostin 6-10B cells. RT-PCR and Western blotting were used to detect the transfection efficiency of 6-10B cells. Tanswell was used to analyze the changes of invasion and migration ability of 6-10B cells after high expression of periostin. The activity of matrix metalloproteinase (MMPs) was detected by gelatinase spectrum. The expression of integrin 伪 v 尾 5 in 6-10B cells before and after periostin transfection and the expression of integrin 伪 v 尾 5 and periostin in nasopharyngeal carcinoma and normal nasopharyngeal mucosa were detected by immunohistochemistry. The correlation between the expression of periostin and integrin 伪 v 尾 5 in nasopharyngeal carcinoma was analyzed statistically. Results the invasion and migration of Periostin overexpression 6-10B cells were significantly enhanced. The activity of MMP-2 and MMP-9 in the supernatant of cell culture increased the expression of integrin 伪 v 尾 5 in periostin overexpressed 6-10B cells and NPC tissues, and the correlation analysis showed that the expression intensity of integrin 伪 v 尾 5 and periostin in NPC tissue was positively correlated with that in NPC tissue. Conclusion exogenous expression of periostin can promote the invasion and metastasis of NPC, and its mechanism may be to increase the activity of MMPs by binding to integrin 伪 v 尾 5 receptor on NPC cell membrane.
【作者单位】：南华大学医学院组织学胚胎学教研室;南华大学医学院细胞分化和组织发生研究室;南华大学护理学院护理研究所;衡阳市中心医院耳鼻喉科;
【基金】：国家自然科学基金资助项目(81072199,81272959) 湖南省自然科学衡阳联合基金资助项目(12JJ9034) 湖南省科技厅资助项目(2011SK3088) 湖南省大学生创新课题资助项目(2012cshncx1)
【分类号】：R739.63

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