HPA、CK2β和HIF-1α在鼻咽癌组织中的表达及意义

发布时间：2018-06-07 08:43

本文选题：鼻咽肿瘤 + 乙酰肝素酶　；参考：《汕头大学》2011年硕士论文

【摘要】：目的: 1、检测乙酰肝素酶、蛋白激酶ck2β、缺氧诱导因子-1α在鼻咽癌组织中的表达以及与临床特征之间的关系。 2、分析乙酰肝素酶、蛋白激酶ck2β、缺氧诱导因子-1α在鼻咽癌组织中表达的相关性。 3、探讨乙酰肝素酶、蛋白激酶ck2β、缺氧诱导因子-1α的表达以及临床特征与鼻咽癌患者预后的关系。材料与方法: 采用免疫组织化学染色技术Super-Vision法检测49例鼻咽癌(NPC)组织标本及15例慢性鼻咽炎(CNT)组织对照标本中HPA、CK2β及HIF-1α的表达,并应用SPSS13.0统计软件包进行相关临床和病理参数分析。结果: 1、HPA在NPC组织及CNT组织中的高表达率分别为49.0%与6.7%,差异有统计学意义(P=0.003)。HPA的表达与NPC临床分期、治疗后有无复发或转移密切相关,不同组别表达水平的差异均有统计学意义(P值分别为0.046、0.001)。HPA的表达与患者的性别、年龄无相关性,不同组别表达水平的差异均无统计学意义。 2、CK2β在NPC组织及CNT组织中的高表达率分别为44.9%与13.2%,差异有统计学意义(P=0.027)。CK2β的表达与NPC临床分期、治疗后有无复发或转移密切相关,不同组别表达水平差异均有统计学意义(P值分别为0.026、0.030)。CK2β的表达与患者的性别、年龄无相关性,不同组别表达水平的差异均无统计学意义。 3、HIF-1α在NPC组织及CNT组织中的高表达率分别为40.8与6.7%,差异有统计学意义(P=0.014)。HIF-1α的表达与NPC患者的年龄、临床分期、治疗后有无复发或转移密切相关,不同组别表达水平差异均有统计学意义(P值分别为0.002、0.027、0.005)。HIF-1α的表达与患者的性别无相关性,不同组别表达水平的差异无统计学意义。 4、HPA与CK2β、HPA与HIF-1α、CK2β与HIF-1α在NPC组织中的表达均呈正相关性(P值分别为0.012、0.006、0.000)。 5、NPC的临床分期、治疗后有无复发或转移与患者的预后密切相关,不同组别比较生存率的差异有统计学意义(P值分别为0.020、0.000)。HPA、CK2β、HIF-1α的表达水平与患者的预后密切相关,不同组别比较生存率的差异有统计学意义(P值分别为0.001、0.019、0.011)。Cox回归模型预后多因素分析显示,仅治疗后有无复发或转移与患者的预后密切相关,不同组别比较生存率的差异有统计学意义(P=0.001)。结论: 1、HPA、CK2β和HIF-1α参与了NPC的生长、浸润及转移,HPA、CK2β和HIF-1α的高表达可促进NPC的发生和发展。 2、在NPC的发生发展过程中,HPA、CK2β和HIF-1α之间可能存在协同作用。 3、临床分期、治疗后有复发或转移以及HPA、CK2β、HIF-1α的表达水平是影响患者预后的因素。 4、治疗后有无复发或转移是影响患者预后的独立危险因素。
[Abstract]:Objective: 1. The expression of heparanase, protein kinase ck2 尾 and hypoxia inducible factor-1 伪 in nasopharyngeal carcinoma (NPC) and the relationship between heparanase, protein kinase ck2 尾 and clinical features were detected. 2. The correlation of heparanase, protein kinase ck2 尾 and hypoxia inducible factor-1 伪 expression in nasopharyngeal carcinoma was analyzed. 3. To investigate the relationship between the expression of heparanase, protein kinase ck2 尾, hypoxia inducible factor-1 伪 and the prognosis of nasopharyngeal carcinoma. Materials and methods: The expression of HPACK2 尾 and HIF-1 伪 in 49 cases of nasopharyngeal carcinoma (NPC) and 15 cases of chronic nasopharyngeal carcinoma (CNT) were detected by immunohistochemical staining Super-Vision method. The clinical and pathological parameters were analyzed by SPSS13.0 statistical software package. Results: 1the high expression rates of HPA in NPC and CNT were 49.0% and 6.7, respectively. The difference was statistically significant. The expression of HPA was closely related to the clinical stage of NPC and the recurrence or metastasis after treatment. There was no correlation between the expression level of HPA and sex and age, but there was no significant difference in expression level between different groups. 2the high expression rates of CK2 尾 in NPC and CNT were 44.9% and 13.2respectively. The difference was statistically significant. The expression of CK2 尾 was closely related to the clinical stage of NPC and the recurrence or metastasis after treatment. There was no correlation between the expression level of CK2 尾 and sex and age, but there was no significant difference in expression level between different groups. 3the high expression rates of HIF-1 伪 in NPC and CNT tissues were 40.8 and 6.7, respectively. The difference was statistically significant. The expression of HIF-1 伪 was closely related to the age, clinical stage, recurrence or metastasis of NPC patients. There was no correlation between the expression of HIF-1 伪 and the sex of the patients, but there was no significant difference in the expression level of HIF-1 伪 between different groups. (4) the expression of HPA and CK2 尾 -HPA and HIF-1 伪, CK2 尾 and HIF-1 伪 were positively correlated in NPC tissues (P = 0.012, P = 0.006, P = 0.000). 5The clinical stage, recurrence or metastasis after treatment were closely related to the prognosis of patients. The difference of survival rate in different groups was significantly different (P = 0.020 0.000). The expression level of HPACK2 尾 -HIF-1 伪 was closely related to the prognosis of patients. The difference of survival rate in different groups was statistically significant (P = 0.001 / 0.019 / 0.011.Cox regression model). Multivariate analysis showed that only recurrence or metastasis after treatment was closely related to the prognosis of the patients. The difference of survival rate in different groups was statistically significant. Conclusion: 1HPA-CK2 尾 and HIF-1 伪 participate in the growth of NPC, and the overexpression of HPA-CK2 尾 and HIF-1 伪 can promote the occurrence and development of NPC. 2. There may be a synergistic effect between HPACK2 尾 and HIF-1 伪 during the development of NPC. 3, clinical stage, recurrence or metastasis after treatment and the expression level of HPA-CK2 尾 -HIF-1 伪 were the prognostic factors. 4. Recurrence or metastasis after treatment is an independent risk factor for prognosis.
【学位授予单位】：汕头大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.6

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