沉默赖氨酰氧化酶基因对喉癌Hep-2细胞凋亡及化疗敏感性的影响

发布时间：2018-06-07 15:59

本文选题：喉肿瘤 + RNA干扰　；参考：《中国现代医学杂志》2014年29期

【摘要】：目的探讨沉默赖氨酰氧化酶(LOX)基因表达对喉鳞癌Hep-2细胞凋亡及化疗敏感性的影响。方法将LOX siRNA转染至Hep-2细胞,免疫细胞化学及免疫印迹(Western blot)检测喉鳞癌Hep-2细胞中LOX蛋白表达情况,实时聚合酶反应(RT-PCR)检测LOX mRNA;MTT法检测不同浓度顺铂对Hep-2细胞生长抑制率的影响;流式细胞术(FCM)检测Hep-2细胞凋亡。结果转染LOX siRNA后Hep-2细胞中LOX蛋白和mRNA表达水平下调(P0.05);用浓度为2、3、4 mg/L顺铂作用Hep-2细胞后,可明显抑制细胞生长(P0.05)。转染组细胞联合顺铂作用后细胞凋亡指数及生长抑制率显著高于阴性对照组、空白对照组、顺铂组及转染组(均P0.05)。结论特异性抑制LOX表达可诱导Hep-2细胞凋亡,并增强喉鳞癌Hep-2细胞对顺铂的敏感性。
[Abstract]:Objective to investigate the effect of silencing lysyl oxidase (LX) gene expression on apoptosis and chemosensitivity of laryngeal squamous cell carcinoma (Hep-2) cells. Methods LOX siRNA was transfected into Hep-2 cells. The expression of LOX protein in laryngeal squamous cell carcinoma (Hep-2) cells was detected by immunocytochemistry and Western blotting. The effect of different concentrations of cisplatin on the growth inhibition of Hep-2 cells was detected by real-time polymerase reaction assay (RT-PCR). Apoptosis of Hep-2 cells was detected by flow cytometry (FCM). Results the expression of LOX protein and mRNA in Hep-2 cells was down-regulated after transfection of LOX siRNA, and the proliferation of Hep-2 cells was significantly inhibited by the treatment of cisplatin with 2G 3O 4 mg/L. The apoptosis index and growth inhibition rate of transfected cells combined with cisplatin were significantly higher than those of negative control group, blank control group, cisplatin group and transfection group (all P 0.05). Conclusion the specific inhibition of LOX expression can induce apoptosis of Hep-2 cells and enhance the sensitivity of Hep-2 cells to cisplatin.
【作者单位】：河北医科大学第一医院耳鼻咽喉头颈外科;
【基金】：河北省医学科学研究重点课题计划项目(No:20120049)
【分类号】：R739.65

【共引文献】