喉癌病变过程中细胞周期调控因子的流式检测及意义

发布时间：2018-06-07 16:11

本文选题：喉肿瘤 + 癌前病变　；参考：《临床耳鼻咽喉头颈外科杂志》2014年09期

【摘要】：目的:探讨细胞周期调控基因cyclin E、cyclin D1、p21、p16在喉癌发生、发展中的作用。方法:选取23例声带息肉、69例喉癌前病变、33例喉癌石蜡包埋组织为研究对象,切片脱蜡后制备成细胞悬液,采用流式细胞术检测cyclin E、cyclin D1、p21、p16基因的荧光表达情况。结果:在声带息肉、喉癌前病变、喉癌组织中,cyclin E的阳性表达率分别为13.04%、20.29%和42.42%;cyclin D1的阳性表达率分别为26.09%、43.48%和93.94%;p16的阳性表达率分别为61.90%、40.98%和14.28%;p21的阳性表达率分别为47.62%、23.81%和26.23%。提示cyclin D1、cyclin E的阳性率表达形成声带息肉→喉癌前病变→喉癌组织阳性强度逐渐增强的梯度现象,而p21、p16的阳性表达强度则呈逐渐减弱的现象。结论:①细胞周期调控因子的异常表达是喉癌发生的分子事件;②正性调控因子cyclin D1、cyclin E高表达,负性调控因子p16、p21低表达,即多个与细胞周期相关的正负调控因子失衡在喉癌的发生中可能起着重要作用。
[Abstract]:Aim: to investigate the role of cell cycle regulation gene cyclin Ecyclin D _ 1 p21 ~ (-1) p16 in the development of laryngeal carcinoma. Methods: the paraffin embedded tissues of 23 cases of vocal cord polyp and 69 cases of laryngeal precancerous lesions were selected as the study objects. After dewaxing, the cell suspension was prepared and the fluorescence expression of cyclin D1p21 p16 gene was detected by flow cytometry. Results: the positive expression rates of cyclin E in vocal cord polyps, laryngeal precancerous lesions and laryngeal carcinomas were 13.04% and 42.42%, respectively. The positive rates of cyclin D1 were 26.099.09%, 43.48% and 93.94%, respectively. The positive rates of cyclin E were 61.9040.98% and 14.28%, respectively. The positive rates of cyclin D1 and cyclin D1 were 47.62% and 26.2381%, respectively. The results suggest that the positive rate of cyclin D _ 1 cyclin E is a gradient phenomenon of the progressive enhancement of the positive intensity in the precancerous lesion of vocal cord polyp, while the positive intensity of p21 ~ + p16 decreases gradually. Conclusion the abnormal expression of cell cycle regulatory factor in 1 cell line is due to the overexpression of cyclin D1 cyclin E, and the low expression of negative regulatory factor p16 p21 in laryngeal carcinogenesis. That is, multiple positive and negative regulatory factors associated with cell cycle may play an important role in laryngeal carcinogenesis.
【作者单位】：广州中医药大学第二附属医院;广东省中医院耳鼻咽喉科;广东省中医药科学院(广东省中医院)中心实验室;广东省中医院病理科;广州中医药大学附属中山医院;中山市中医院耳鼻咽喉科;
【基金】：国家自然科学基金(No:30873288)
【分类号】：R739.65

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