FA-MNP-MMP-9-ASODN纳米复合物对鼻咽癌的体外抑制研究

发布时间：2018-06-12 13:42

本文选题：鼻咽肿瘤 + 基质金属蛋白酶　；参考：《临床耳鼻咽喉头颈外科杂志》2014年09期

【摘要】：目的:观察FA-MNP-MMP-9-ASODN纳米复合物对鼻咽癌HNE-1细胞的体外抑制效应。方法:采用RT-PCR、Western-blot方法、MTT方法、流式细胞技术及Matrigel体外侵袭实验观察转染FA-MNP-MMP-9-ASODN 48h后HNE-1细胞MMP-9mRNA及蛋白表达水平和细胞增殖能力、凋亡和侵袭能力的变化。结果:FA-MNP-MMP-9-ASODN组HNE-1细胞MMP-9 mRNA和蛋白的表达水平较对照组及FA-MNP-MMP-9-NSODN无义序列组明显降低。同时FA-MNP-MMP-9-ASODN组HNE-1细胞生长抑制率约为35.66%,增殖活性较对照组和无义序列组明显降低。且HNE-1细胞周期也受到抑制,细胞凋亡率约为12.60%,明显高于对照组和无义序列组。侵袭实验显示FA-MNP-MMP-9-ASODN组穿膜细胞数约为21.00个,明显低于对照组和无义序列组。结论:FA-MNP-MMP-9-ASODN纳米复合物通过抑制MMP-9的表达,可降低鼻咽癌细胞的增殖和侵袭能力,促进凋亡,体外抑制效果良好。
[Abstract]:Aim: to investigate the inhibitory effect of FA-MNP-MMP-9-ASODN nanocomposite on nasopharyngeal carcinoma HNE-1 cells in vitro. Methods: MTT assay, flow cytometry and Matrigel invasion assay were used to observe the changes of MMP-9 mRNA and protein expression, cell proliferation, apoptosis and invasion ability of HNE-1 cells transfected with FA-MNP-MMP-9-ASODN 48 h after transfection. Results the expression of MMP-9 mRNA and protein in HNE-1 cells in the group of 10% FA-MNP-MMP-9-ASODN was significantly lower than that in the control group and FA-MNP-MMP-9-NSODN group. At the same time, the growth inhibition rate of HNE-1 cells in FA-MNP-MMP-9-ASODN group was about 35.66, and the proliferative activity was significantly lower than that in control group and nonsense sequence group. The cell cycle of HNE-1 was also inhibited, the apoptosis rate was about 12.60%, which was significantly higher than that of control group and nonsense sequence group. Invasion test showed that the number of perforating cells in FA-MNP-MMP-9-ASODN group was 21.00, which was significantly lower than that in control group and nonsense sequence group. Conclusion by inhibiting the expression of MMP-9, the cell proliferation and invasion of nasopharyngeal carcinoma cell line can be reduced, and apoptosis can be promoted by the nano-complex: FA-MNP-MMP-9-ASODN. The inhibition effect in vitro is good.
【作者单位】：广州南方医科大学珠江医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金资助项目(No:81372477,No:81260406) 教育部高等学校博士学科点专项科研基金(No:20114433110001)
【分类号】：R739.63

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