慢性中耳炎细菌生物膜形成与细菌培养结果的相关性分析

发布时间：2018-06-14 19:07

本文选题：中耳炎 + 生物膜　；参考：《临床耳鼻咽喉头颈外科杂志》2014年13期

【摘要】：目的:探讨慢性中耳炎细菌生物膜形成与细菌培养之间的相关性。方法:对32例慢性化脓性中耳炎及中耳胆脂瘤患者术中取得的样本进行扫描电镜检查,并对中耳分泌物行细菌培养;分析慢性中耳炎细菌生物膜形成与细菌培养结果之间的关系。结果:慢性化脓性中耳炎(活动期)与中耳胆脂瘤细菌生物膜形成率分别为87.5%、81.3%(P0.05)。慢性中耳炎扫描电镜细菌生物膜结果与细菌培养结果之间比较,灵敏度为70.37%,特异度为60.00%,误诊率为40.00%,漏诊率为29.63%,阳性预测值为90.46%,阴性预测值为27.27%,正确率为68.75%,约登指数为30.37%,Pearson相关系数为0.232(P0.05)。结论:慢性化脓性中耳炎(活动期)与中耳胆脂瘤均有较高的生物膜形成率,但中耳分泌物常规细菌培养结果不能反应慢性中耳炎患者细菌生物膜的形成情况,需要探索更为可靠的细菌学实验方法来准确反映慢性中耳炎的感染情况。
[Abstract]:Objective: to investigate the relationship between bacterial biofilm formation and bacterial culture in chronic otitis media. Methods: the samples obtained from 32 patients with chronic suppurative otitis media and cholesteatoma were examined by scanning electron microscope, and the secretion of middle ear was cultured. To analyze the relationship between bacterial biofilm formation and bacterial culture results in chronic otitis media. Results: the biofilm formation rates of chronic suppurative otitis media (active stage) and middle ear cholesteatoma were 87.5 and 81.3% respectively. The results of bacterial biofilm and bacterial culture in chronic otitis media were compared with those in scanning electron microscope. The sensitivity was 70.37 and the specificity was 60.00. the misdiagnosis rate was 40.00. the missed diagnosis rate was 29.63. The positive predictive value was 90.46 and the negative predictive value was 27.27. The accuracy was 68.75. The Pearson correlation coefficient was 0.232P 0.05. Conclusion: both chronic suppurative otitis media (active stage) and middle ear cholesteatoma have high biofilm formation rate, but the bacterial biofilm formation in patients with chronic otitis media can not be reflected by the results of routine bacterial culture of middle ear secretion. A more reliable bacteriological method is needed to accurately reflect the infection of chronic otitis media.
【作者单位】：新疆维吾尔自治区人民医院耳鼻咽喉二科;新疆医科大学第一附属医院;新疆医科大学第一附属医院耳鼻咽喉科;
【基金】：新疆维吾尔自治区人民医院院内资助项目(No:20110121)
【分类号】：R764.21

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