人乳头状瘤病毒DNA及其亚型在喉癌、喉咽癌中的表达及临床意义

发布时间：2018-06-18 05:06

本文选题：喉肿瘤 + 癌　；参考：《青岛大学》2013年硕士论文

【摘要】：目的通过检测喉鳞状细胞癌(鳞癌)肿瘤细胞中人乳头状瘤病毒(Human papillo-mavirus,HPV)的DNA及其亚型的表达,探讨HPV感染与喉鳞癌的关系及在喉鳞癌发病中的意义。方法收集2011年12月-2013年03月间,青岛大学医学院附属医院耳鼻咽喉头颈外科初治喉癌患者82例,男性80例,女性2例,37-83岁,平均(60.3±9.6)岁,均经病理检查诊断为喉鳞状细胞癌,无喉乳头状瘤恶变者。对照组为同期喉乳头状瘤患者8例,均为男性,51-61岁,平均(57.8±3.2)岁,术后均经病理检查证实为喉乳头状瘤。将术中切除的新鲜病变组织以棉拭子刷取肿瘤组织细胞,并存放至细胞保存液中。采用聚合酶链式反应(Polymerase Chain Reaction,PCR)扩增和DNA反向点杂交相结合的DNA芯片技术,检测两组细胞HPV DNA及其亚型的表达。结果喉癌82例HPV DNA检出者5例,喉乳头状瘤组8例检出者6例。喉癌组HPVDNA捡出率为6.10%(5/82)较喉乳头状瘤组75.00%(6/8)低,差异具有极显著性意义(X2=26.15,P=0,P＜0.01)。喉癌组高危型HPV16DNA检出者3例,占3.66%,低危型HPV11DNA与HPV43DNA各1例,分别占1.22%。喉乳头状瘤组HPV16DNA捡出者2例,占25.00%.HPV11、HPV6DNA各2例,分别占25.00%。喉癌组高危型HPV16DNA与低危型HPV43DNA检出率分别与喉乳头状瘤组比较,差异均无显著性意义(P=0.061、1.0,P＞0.05)。喉乳头状瘤组低危型HPV11DNA与HPV6DNA检出率较喉癌组高,差异均有显著性意义(P=0.02、0.007,P0.05)。结论本研究观察到HPV感染与喉鳞癌有关,HPV DNA检出率为6.1%;HPV DNA阳性的喉鳞癌患者中以高危型HPV16为主,占60%。目的通过检测喉咽鳞状细胞癌(喉咽癌)中人乳头状瘤病毒(HPV)的DNA及其亚型的表达,探讨HPV感染与喉咽癌的关系及在喉咽癌发病中的意义。方法收集2011年12月-2013年03月间,青岛大学医学院附属医院耳鼻咽喉头颈外科初治喉咽癌患者36例,男性35例,女性1例,42-77岁,平均(57.4±8.2)岁,均经病理检查诊断为喉咽鳞状细胞癌,术中以棉拭子刷取肿瘤细胞。对照组患者35例,男性32例,女性3例,42-75岁,平均(55.1±8.1)岁,其中声带息肉患者26例,会厌囊肿患者9例,术中以棉拭子刷取喉咽部黏膜脱落细胞。采用聚合酶链式反应(PCR)扩增和DNA反向点杂交相结合的DNA芯片技术,检测两组细胞HPV DNA及其亚型的表达。结果喉咽癌组36例中HPV DNA检出者6例,HPV DNA检出率16.7%(6/36)与对照组(0%)比较,差异有显著性意义(x2=4.40,P=0.036,P0.05).喉咽癌组中高危型HPV DNA检出者5例,占83.3%,其中HPV16DNA2例,HPV18、59、66DNA各1例；低危型HPV DNA检出者仅有1例,为HPV43。喉咽癌组高危型HPV DNA检出者与对照组比较,差异无显著性意义(x2=3.323,P=0.068,P0.05).喉咽癌组低危型HPV DNA检出者与对照组比较,差异亦无显著性意义(P=1.00,P0.05). 结论本研究观察到HPV感染与喉咽鳞癌有关,HPV DNA捡出率为16.7%；HPVDNA阳性的喉咽鳞癌患者中以高危型HPV为主,占83.3%。
[Abstract]:Objective to investigate the relationship between HPV infection and laryngeal squamous cell carcinoma (LSCC) and its significance in the pathogenesis of laryngeal squamous cell carcinoma (LSCC) by detecting the expression of human papillo-mavirusus (HPVV) DNA and its subtypes in human laryngeal squamous cell carcinoma (LSCC). Methods from December 2011 to March 2013, 82 patients with primary laryngeal carcinoma in the Department of Otorhinolaryngology and head and neck surgery, affiliated Hospital of Qingdao University, were collected, including 80 males and 2 females aged 37-83 years, with an average age of 60.3 卤9.6 years. All patients were diagnosed as laryngeal squamous cell carcinoma by pathological examination. There was no malignant change in laryngeal papilloma. There were 8 cases of laryngeal papilloma in the control group, all of them were male (51-61 years old, mean 57.8 卤3.2) years old. All cases were proved to be laryngeal papilloma by pathological examination. The tumor cells were removed by cotton swab and stored in the cell preservation solution. DNA microarray was used to detect the expression of HPV DNA and its subtypes in two groups of cells by polymerase chain reaction (PCR) amplification and DNA reverse dot hybridization. Results HPV DNA was detected in 5 cases in 82 cases of laryngeal carcinoma and 6 cases in 8 cases of laryngeal papilloma. The DNA extraction rate of HPV in laryngeal carcinoma group was 6.10 / 82, which was significantly lower than that in laryngeal papilloma group (75.00 / 6 / 8), and the difference was significant (P < 0.01). High risk HPV 16 DNA was detected in 3 cases (3.66%) in laryngeal carcinoma group, and 1 case (1.22%) in low risk type HPV11 DNA and 1 case HPV43 DNA respectively. In the laryngeal papilloma group, 2 cases of HPV16 DNA were picked out, 2 cases of HPV16 DNA were found in 25.00 cases, 2 cases of HPV11 HPV6 DNA were found in the laryngeal papilloma group, accounting for 25.00% of the DNA, respectively. The detection rate of high risk HPV16 DNA and low risk HPV 43 DNA in laryngeal carcinoma group was not significantly different from that in laryngeal papilloma group (P > 0.05). The detection rate of HPV11 DNA and HPV6 DNA in laryngeal papilloma group was higher than that in laryngeal carcinoma group (P < 0.05). Conclusion in this study, we observed that the detection rate of HPV DNA in laryngeal squamous cell carcinoma associated with HPV infection was 6.1 and HPV16 was the most common type in laryngeal squamous cell carcinoma, accounting for 60 cases. Objective to investigate the relationship between human papillomavirus (HPV) infection and laryngeal carcinoma by detecting the expression of HPVDNA and its subtypes in laryngeal squamous cell carcinoma (LSCC) and its significance in the pathogenesis of laryngopharyngeal carcinoma. Methods from December 2011 to March 2013, 36 patients with laryngopharynx carcinoma treated in the Department of Otorhinolaryngology and neck surgery, affiliated Hospital of Qingdao University Medical College, were collected, including 35 males and 1 female, aged 42-77 years, with an average of 57.4 卤8.2 years old. All patients were diagnosed as laryngopharyngeal squamous cell carcinoma by pathological examination. The tumor cells were removed with cotton swab during operation. 35 patients in the control group, 32 males and 3 females aged 42-75 years, with an average age of 55.1 卤8.1 years, including 26 patients with vocal cord polyps and 9 patients with epiglottic cyst. DNA microarray was used to detect the expression of HPV DNA and its subtypes in two groups of cells by polymerase chain reaction (PCR) amplification and reverse dot hybridization. Results the detection rate of HPV DNA in 6 out of 36 patients with laryngopharyngeal carcinoma was 16. 7 / 36), which was significantly higher than that in the control group (P 0. 05). High risk HPV DNA was detected in 5 cases (83.3%) in laryngopharyngeal carcinoma group, including 2 cases of HPV16 DNA, 1 case of HPV1859.66 DNA, and 1 case of low risk type HPV DNA (HPV43). There was no significant difference in high risk HPV DNA detection between the larynx carcinoma group and the control group. There was no significant difference between the low risk HPV DNA detection group and the control group. Conclusion in this study, the detection rate of HPV DNA in larynx squamous cell carcinoma associated with HPV infection was 16.7% (83.3%) in patients with HPV DNA positive larynx pharynx squamous cell carcinoma.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R739.6

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