光照强度对豚鼠屈光发育的影响及视网膜多巴胺变化研究

发布时间：2018-06-22 20:51

本文选题：光照强度 + 屈光发育　；参考：《复旦大学》2011年硕士论文

【摘要】：目的：探讨豚鼠在不同光强度照明条件下(5Lux,500Lux,10000 Lux,白色光,色温6000K)的屈光发育状况,以比较光强度对豚鼠屈光发育的影响,并检测视网膜多巴胺含量变化,初步探讨光照强度影响屈光发育的可能机制。方法：30只3周龄的健康豚鼠(英国种三色豚鼠),随机分为强光组10只、弱光组10只和正常对照组10只,分别置于10000Lux,5Lux,500Lux三种光照强度下,光照周期为12/12h(早6：00-晚6：00)。于实验前及光照12周末分别用带状检影计、A超测定仪、角膜曲率计对豚鼠右眼重复进行眼球的生物学测量(包括屈光度、眼轴、角膜曲率)。光照12周结束后处死豚鼠取右眼球行高效液相色谱(HPLC)分析以及光镜电镜下行后极眼球壁结构观察。对不同时间点的组间测量数据采用单因素方差分析,以P0.05为差异有统计学意义。结果：光照前不同组间生物学测量参数差异无显著性(P0.05)。光照12周后,强光组屈光度为4.03±1.59D,同光照前相比发生0.45±1.65D的变化,正常对照组屈光度为2.15±2.O1D,发生2.28±0.66D的变化,强光组同正常对照组相比远视度数偏高约1.501),两者差异有显著性(P0.05)；强光组眼轴增长0.54±0.10mm,正常对照组为0.76±0.05mm,强光组较对照组眼轴长度延长较慢,差异有显著性(P0.05)；光照后不同组角膜曲率半径均增加,但组间变化差异无显著性(P0.05)。强光组视网膜多巴胺含量平均为148.70±22.44 nmol/g,正常对照组为44.50±12.45 nmol/g,两者差异有显著性(P0.01)。光照12周后弱光组较正常对照组相比,无论是屈光度、曲率、眼轴以及视网膜多巴胺含量均无统计学差异(P0.05)。光镜下见强光组后极部巩膜层较正常光照组厚。电镜下见强光组光感受器部分外节膜盘间隙增宽,内节部分线粒体肿胀,嵴脱落,余未见明显异常。结论：高强度光照可以引起豚鼠眼球眼轴增长减缓,正视化进展减慢,屈光度数偏远视,弱光对豚鼠的屈光发育没有影响。强光照射后可以引起豚鼠视网膜多巴胺含量增加,可能为强光引起豚鼠正视化进展减缓的机制之一。强光对豚鼠视网膜光感受器造成轻微损伤。
[Abstract]:Objective: to study the refractive development of guinea pigs under different light intensity illumination (5Lux500Lux10000 Lux10000, white light, color temperature 6000K), to compare the effect of light intensity on refractive development of guinea pigs, and to detect the changes of dopamine content in retina. To explore the possible mechanism of light intensity affecting refractive development. Methods Thirty 3-week-old healthy guinea pigs (British tricolor guinea pigs) were randomly divided into three groups: high light group (n = 10), weak light group (n = 10) and normal control group (n = 10). Under three light intensities of 10000Lux5Lux500Lux, the period of illumination was 12 / 12h (6: 00 to 6:00). The biologic measurements of the right eye of guinea pig (including diopter, axis, cornea curvature) were carried out before the experiment and at the end of 12 weeks of illumination by using the band detector and keratometer respectively. At the end of 12 weeks of illumination, the right eye balls of the guinea pigs were sacrificed for HPLC analysis and for observation of the wall structure of the posterior polar eyeball under light microscope and electron microscope. Single factor analysis of variance (ANOVA) was used to measure the data at different time points, and the difference was statistically significant (P0.05). Results: there was no significant difference in biological parameters between different groups before illumination (P0.05). After 12 weeks of illumination, the diopter of the high-light group was 4.03 卤1.59D, which was 0.45 卤1.65D compared with that of the control group, and the diopter of the control group was 2.15 卤2.O1D and 2.28 卤0.66D, and the hyperopia degree of the high-light group was higher than that of the control group (about 1.501), the difference was significant (P0.05). The axial length of the eyes in the strong light group was 0.54 卤0.10 mm, and that in the normal control group was 0.76 卤0.05 mm. The length of the axial axis in the strong light group was longer than that in the control group (P0.05), and the corneal radius of curvature was increased in the different groups after illumination (P0.05). The average dopamine content was 148.70 卤22.44 nmol / g in the high-light group and 44.50 卤12.45 nmol / g in the normal control group. There was a significant difference between the two groups (P0.01). There was no significant difference in diopter curvature eye axis and retinal dopamine content between the low light group and the normal control group after 12 weeks of illumination (P0.05). Under light microscope, the posterior sclera of the strong light group was thicker than that of the normal light group. Under the electron microscope, the outer segment of the photoreceptor in the strong light group was enlarged, the mitochondria of the inner ganglia was swollen, the ridge was abscission, and no obvious abnormality was found in the rest. Conclusion: high intensity illumination can slow down the growth of eye axis, slow down the progress of emmetropia, and have no effect on the development of refraction in guinea pigs. The dopamine content in the retina of guinea pigs increased after strong light irradiation, which may be one of the mechanisms of slowing down the progress of emmetropia caused by intense light. Strong light caused slight damage to retinal photoreceptors in guinea pigs.
【学位授予单位】：复旦大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R778

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