环磷腺苷效应元件结合蛋白磷酸化在胰岛素样生长因子-1抑制耳蜗毛细胞凋亡中的作用

发布时间：2018-06-22 21:42

本文选题：毛细胞 + 凋亡　；参考：《中国老年学杂志》2015年12期

【摘要】：目的探讨磷酸化环磷腺苷效应元件结合蛋白(P-CREB)对胰岛素样生长因子(IGF)-1抑制小鼠毛细胞凋亡的作用。方法体外培养新生小鼠耳蜗基底膜。实验分为A组:正常对照培养液;B组:庆大霉素(GM)(0.5 mmol/L);C组:GM(0.5 mmol/L)+IGF-1(1 ng/ml);D组:GM(0.5 mmol/L)+IGF-1(10 ng/ml);E组GM(0.5 mmol/L)+IGF-1(50 ng/ml);F组:GM(0.5 mmol/L)+IGF-1(100 ng/ml)。培养24 h后收集细胞及固定。应用TUNEL法观察各组耳蜗毛细胞的凋亡情况。应用Western印迹及RT-PCR法观察P-CREB的表达情况。结果 A组无凋亡细胞。B组检测到凋亡细胞。C组细胞凋亡较B组明显减少。D组凋亡细胞减少最明显(与B组相比,P0.05)。E组及F组凋亡细胞较D组有所增加。P-CREB:Western印迹结果及RT-PCR结果表明:A组及B组P-CREB蛋白及m RNA表达较低。与B组相比,C、D、E及F组P-CREB蛋白及m RNA明显增加,以D组增加最明显。结论 IGF可能通过激活CREB磷酸化抑制毛细胞凋亡。
[Abstract]:Objective to investigate the effect of P-CREB on the inhibition of mouse hair cell apoptosis by insulin like growth factor (IGF) -1. Methods the cochlear basement membrane was cultured in vitro. The experiment was divided into A group: normal control medium; B group: gentamicin (GM) (0.5 mmol/L); C group: GM (0.5 mmol/L) +IGF-1 (1 ng/ml); 0 .5 mmol/L) +IGF-1 (10 ng/ml); E group GM (0.5 mmol/L) +IGF-1 (50 ng/ml); F group: GM (0.5 mmol/L) (100). Cells and immobilization were collected after cultivation 24. Apoptotic cells in group.C group were significantly lower than that in group B (compared with group B, compared with group B, P0.05), and the apoptotic cells in group.E and F group increased.P-CREB:Western blot results and RT-PCR results showed that A group and B group were significantly higher than those in D group. Conclusion IGF may inhibit the apoptosis of hair cells by activating CREB phosphorylation.
【作者单位】：辽宁医学院附属第一医院耳鼻咽喉科;辽宁医学院附属第一医院风湿科;
【基金】：辽宁省教育厅高等学校科研项目(2014A460)
【分类号】：R764

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