益生菌提取物LFK对变应性鼻炎小鼠模型脾脏组织中CD80和CD86表达的影响

发布时间：2018-06-23 22:31

本文选题：变应性鼻炎 + 益生菌　；参考：《南京医科大学》2014年硕士论文

【摘要】：背景：虽然人们已普遍认识到摄入益生菌(probiotics)这种微生物有利于改善慢性黏膜炎症和变态反应性疾病,但是这类微生物如何产生这些免疫学上的改变仍不十分清楚。目的：对变应性鼻炎(allergic rhinitis, AR)小鼠模型经口途径分别给予酶裂解的肠球菌FK-23株(lysed Enterococcus faecalis FK-23, LFK),观测小鼠脾脏内抗原提呈细胞(antigen-presenting cells, APC)表面组织中共刺激分子CD80和CD86的表达改变,评价益生菌对实验性AR的免疫调节作用。方法：共计75只BALB/c小鼠随机分为三组：①阳性对照组；②LFK干预组；③阴性对照组。采用OVA腹腔注射及鼻部激发建立小鼠AR模型,阴性对照组小鼠采用生理盐水代替OVA进行腹腔注射及滴鼻。用LFK菌液分别对AR小鼠模型进行灌胃,共计3周；阳性和阴性对照组以生理盐水代替菌液灌胃处理。观测第21天小鼠搔鼻和喷嚏次数。第22天杀鼠取材,小鼠脾脏组织用免疫组织化学法检测APC表面共刺激分子CD80和CD86的表达。并采用酶联免疫吸附试验(enzyme-linked immunosorbent assay, ELISA)检测小鼠血清中细胞因子IL-12水平。结果：第21天小鼠搔鼻和喷嚏次数,阳性对照组及LFK干预组明显高于阴性对照组(P0.05),LFK干预组明显低于阳性对照组(P0.05)。血清IL-12水平阳性对照组较阴性对照组显著降低(P0.001),LFK干预组较阳性对照组显著升高(P0.001),但与阴性对照组相比仍明显降低(P0.001)。小鼠脾脏APC表面共刺激分子组织中CD80的表达,阳性对照组明显低于阴性对照组及LFK干预组(P0.05),LFK干预组与阴性对照组相比差异无统计学意义(P0.05)；CD86的表达,阳性对照组明显高于阴性对照组及LFK灌胃干预组(P0.05),LFK干预组与阴性对照组相比差异无统计学意义(P0.05)。结论：在小鼠AR模型中,经口途径使用益生菌制剂品LFK可明显减轻小鼠鼻部变应性症状,改变小鼠脾脏细胞表面组织中共刺激分子CD80和CD86的表达,并对细胞因子血清IL-12水平具有一定的调节作用。
[Abstract]:Background: although it has been widely recognized that ingestion of probiotic (probiotics) is beneficial to the improvement of chronic mucosal inflammation and allergic diseases, it is not clear how these microbes produce these immunological changes. Objective: to observe the expression of costimulatory molecules CD80 and CD86 on the surface of (allergic rhinitis, cells (antigen-presenting cells) of lysed Enterococcus faecalis FK-23 (LFK). To evaluate the immunomodulatory effect of probiotics on experimental AR. Methods: a total of 75 BALB / c mice were randomly divided into three groups. The AR model of mice was established by OVA intraperitoneal injection and nasal stimulation. The mice in negative control group were treated with saline instead of OVA for intraperitoneal injection and nasal drip. The AR mouse model was perfused with LFK bacteria solution for 3 weeks, and the positive and negative control groups were treated with normal saline instead of bacteria solution. The times of nose scratching and sneezing were observed on day 21. The expression of costimulatory molecules CD80 and CD86 on the surface of APC was detected by immunohistochemical method. The level of cytokine IL-12 in serum of mice was detected by enzyme-linked immunosorbent assay (Elisa). Results: the times of nose scratching and sneezing were significantly higher in the positive control group and LFK intervention group than in the negative control group on the 21st day (P0.05) and lower in the LFK intervention group than in the positive control group (P0.05). The level of serum IL-12 in the positive control group was significantly lower than that in the negative control group (P0.001). The serum IL-12 level in LFK intervention group was significantly higher than that in the positive control group (P0.001), but it was still significantly lower than that in the negative control group (P0.001). The expression of CD80 was significantly lower in the positive control group than that in the negative control group and LFK intervention group (P0.05). There was no significant difference in CD86 expression between the LFK intervention group and the negative control group (P0.05). The positive control group was significantly higher than the negative control group and LFK gastric perfusion intervention group (P0.05) there was no significant difference between LFK intervention group and negative control group (P0.05). Conclusion: in mouse AR model, the oral administration of probiotic preparation LFK can significantly reduce the nasal allergic symptoms of mice, and change the expression of CD80 and CD86 on the surface of spleen cells in mice. And it can regulate the level of IL-12 in serum of cytokines.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R765.21

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