去整合素Echistatin对糖尿病兔后发性白内障模型中晶状体上皮细胞间质转分化及胶原合成的影响

发布时间：2018-06-25 19:30

  本文选题：后囊膜混浊 + 糖尿病　； 参考：《眼科新进展》2014年04期

【摘要】：目的观察去整合素Echistatin对糖尿病兔晶状体摘出术后后囊膜混浊(posterior capsular opacification,PCO)的影响,探讨Echistatin对晶状体上皮细胞(lens epithelial cells,LEC)间质转分化及胶原合成的抑制作用。方法应用四氧嘧啶耳缘静脉注射建立糖尿病兔模型,随机分组并行晶状体摘出术,术毕前房分别注入0.2 mL高压灭菌蒸馏水(对照组),7.5 mg·L-1Echistatin(7.5 mg·L-1组)、10.0 mg·L-1Echistatin(10.0 mg·L-1组),每组又分为10 d和6周2个时间点(n=4)。裂隙灯显微镜观察各组糖尿病兔术后10 d和6周PCO分级情况,同时摘取术眼应用RT-PCR法检测晶状体后囊膜上α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)和Ⅳ型胶原mRNA表达情况。结果术后10 d,各组间α-SMA mRNA和IV型胶原mRNA表达差异均无统计学意义(均为P0.05)。术后6周,Echistatin干预组PCO分级均明显低于对照组(P=0.024);7.5 mg·L-1Echistation组α-SMA mRNA表达为3.97±0.76,对照组为9.06±3.40,10.0 mg·L-1组为6.80±3.15,7.5 mg·L-1组明显低于其余两组(P=0.002、0.024);Ⅳ型胶原mRNA表达对照组为30.88±20.13,7.5 mg·L-1组为8.62±6.99,10.0 mg·L-1组为5.76±6.73,Echistatin干预组Ⅳ型胶原mRNA表达均低于对照组(P=0.005、0.002)。结论去整合素Echistatin对糖尿病兔晶状体摘出术后PCO的发生和发展有一定的抑制作用,可能与其抑制晶状体后囊膜上α-SMA和Ⅳ型胶原的mRNA表达有关。
[Abstract]:Objective to observe the effect of Echistatin on (posterior capsular opacification of posterior capsular opacification after lens extraction in diabetic rabbits, and to investigate the inhibitory effect of Echistatin on interstitial transdifferentiation and collagen synthesis of lens epithelial cells (lens epithelial cells). Methods Diabetic rabbit model was established by intravenous injection of alloxan into ear margin. At the end of the operation, 0.2 mL high pressure sterilized distilled water (control group) was injected with 7.5 mg L -1 Echistatin (7.5 mg L -1 group) and 10.0 mg L -1 Echistatin (10.0 mg L -1 group) respectively. Each group was divided into two time points (nun4) for 10 days and 6 weeks. Slit-lamp microscope was used to observe the grade of PCO in diabetic rabbits 10 and 6 weeks after operation. The expression of 伪 -smooth muscle actin (伪 -SMA) and type 鈪，

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