根皮素对糖尿病小鼠光感受器视杆细胞的保护作用

发布时间：2018-06-27 23:30

本文选题：根皮素 + 糖尿病视网膜病变　；参考：《眼科新进展》2014年09期

【摘要】：目的观察根皮素对糖尿病小鼠视网膜视杆细胞功能和形态的影响。方法健康8周龄C57BL/6小鼠随机分为正常对照组、糖尿病对照组和根皮素治疗组,每组6只。采用腹腔注射链脲佐菌素50mg·kg-1连续5d建立糖尿病小鼠模型,成模后根皮素治疗组予以根皮素100mg·kg-1灌胃,连续12周,正常对照组和糖尿病对照组予以等量生理盐水。成模18周后3组小鼠行暗适应视网膜电图检查视杆细胞功能,并测量视网膜含糖量以及行HE染色切片测量距离视盘上下方0.48mm、0.96mm、1.44mm、1.92mm处视网膜外核层厚度检查视杆细胞形态学变化。结果糖尿病模型成模后18周糖尿病对照组视网膜含糖量为(142.25±6.92)nmol glucose/mg protein、根皮素治疗组视网膜含糖量为(97.82±4.12)nmol glucose/mg protein,均高于正常对照组(39.43±2.17)nmol glucose/mg protein,但根皮素治疗组视网膜含糖量比糖尿病对照组低,差异有显著统计学意义(P0.01)。暗适应视网膜电图发现糖尿病对照组a波及b波振幅为(125.6±19.6)μV、(264.3±21.5)μV,根皮素治疗组为(226.7±23.2)μV、(515.3±36.4)μV,均低于正常对照组(359.3±32.3)μV、(830.8±45.2)μV,但根皮素治疗组比糖尿病对照组高,差异均有显著统计学意义(均为P0.01)。根皮素治疗组和糖尿病对照组小鼠视网膜外核层厚度较正常对照组下降(6.28~16.05)%和(20.41~35.38)%,然而根皮素治疗组视网膜外核层厚度较糖尿病对照组小鼠厚(6.61~29.92)%,差异有统计学意义(P0.05)。结论根皮素通过抑制葡萄糖转运蛋白-1转运葡萄糖进入视网膜,限制视网膜局部含糖量,从而对光感受器视杆细胞的功能和形态均产生保护作用。
[Abstract]:Objective to observe the effect of phylloderm on the function and morphology of retinal rod cells in diabetic mice. Methods healthy 8 week old C57BL / 6 mice were randomly divided into normal control group, diabetic control group and root skin therapy group with 6 mice in each group. The diabetic mice model was established by intraperitoneal injection of streptozotocin 50mg kg-1 for 5 days. After the model was established, the rats in the treated group were fed with 100mg kg-1 for 12 weeks, and the normal control group and the diabetic control group were given the same amount of normal saline. After 18 weeks of modeling, dark adaptation electroretinogram was used to examine the cell function of the rod, and the retinal sugar content was measured. The thickness of the outer nuclear layer of the retina was measured by HE staining at the distance of 0.48 mm ~ 0.96 mm ~ 1.44 mm ~ 1.92 mm from the upper and lower side of the optic disc. The thickness of the outer nuclear layer of the retina was measured to examine the morphological changes of the rod cell. Results the content of glucose in retina of diabetic control group was (142.25 卤6.92) nmol glucose/mg at 18 weeks after diabetic model, and that of retina was (97.82 卤4.12) nmol glucose/mg in dermatocutin-treated group, which was higher than that in normal control group (39.43 卤2.17) nmol glucose/mg protein, but the glucose content in retina of treated group was lower than that in diabetic control group. The difference was statistically significant (P0.01). The amplitudes of a wave and b wave were (125.6 卤19.6) 渭 V, (264.3 卤21.5) 渭 V in diabetic control group, (226.7 卤23.2) 渭 V, (515.3 卤36.4) 渭 V in root dermatotin group, which were lower than those in normal control group (359.3 卤32.3) 渭 V and (830.8 卤45.2) 渭 V, respectively. Compared with the normal control group, the thickness of the outer nuclear layer of the retina decreased (6.28 ~ 16.05)% and (20.41 卤35.38), but the thickness of the outer nuclear layer of the retina in the treated group was thicker than that in the diabetic control group (6.61 卤29.92), the difference was statistically significant (P0.05). Conclusion by inhibiting glucose transport of glucose transporter 1 into the retina and limiting the local glucose content in the retina, phloderin has protective effects on the function and morphology of the photoreceptor rod cells.
【作者单位】：南昌大学第二附属医院眼科;
【基金】：江西省教育厅青年科学基金(编号:GJJ13046)~~
【分类号】：R587.2;R774.1

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