庆大霉素耳毒性模型大鼠耳蜗凋亡诱导因子表达及阿司匹林的拮抗作用

发布时间：2018-06-29 20:56

本文选题：阿司匹林 + 庆大霉素　；参考：《听力学及言语疾病杂志》2014年04期

【摘要】：目的研究庆大霉素耳毒性模型大鼠耳蜗凋亡诱导因子(apoptosis-inducing factor,AIF)表达及阿司匹林的拮抗作用。方法 20只SD大鼠随机分为阿司匹林(acetylsalicylic acid,ASA)+庆大霉素(GM)组(ASA+GM组)和庆大霉素组(GM组),每组各10只动物,GM组腹腔注射GM 120mg·kg-1·d-1,连续21天,ASA+GM组先给予ASA 100mg·kg-1·d-1灌胃,1小时后皮下注射等剂量GM,连续21天;两组动物分别于用药前、用药第7、14、21d行ABR检测,并于用药21d后进行耳蜗毛细胞计数及免疫荧光染色观察两组大鼠耳蜗AIF表达。结果用药第7、14、21dASA+GM组ABR反应阈均低于GM组(P0.05);用药21d时ASA+GM组耳蜗毛细胞缺失率为7.20%,明显低于GM组(21.51%)(P0.05),且在两组耳蜗毛细胞亡处均见AIF高表达。结论阿司匹林能拮抗庆大霉素的耳毒性,庆大霉素损伤耳蜗外毛细胞时AIF参与了细胞的凋亡。
[Abstract]:Objective to study the expression of apoptosis-inducing factor AIF and the antagonistic effect of aspirin on gentamicin ototoxicity in rat cochlea. Methods Twenty SD rats were randomly divided into gentamycin (GM) group and gentamicin group (GM group). 10 rats in each group were intraperitoneally injected with GM 120mg kg-1 d-1. Subcutaneous injection of equal dose of GM for 21 days; ABR was detected on day 714 and 21, and the expression of AIF in cochlea of the two groups was observed by immunofluorescence staining and counting of hair cells in cochlea. Results the ABR response threshold of ASA GM group was lower than that of GM group (P0.05), and the loss rate of cochlear hair cells in ASA GM group was 7.20%, which was significantly lower than that in GM group (21.51%) (P0.05). Conclusion AIF can antagonize the ototoxicity of gentamicin, and AIF is involved in the apoptosis of the cochlear outer hair cells.
【作者单位】：第四军医大学西京医院全军耳鼻咽喉-头颈外科专科中心;第四军医大学西京医院神经内科;
【基金】：国家“973”项目(2011CB504505) 国际(地区)合作与交流项目(81120108008)资助
【分类号】：R764

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