氧化应激上调IL-17在Graves眼病的研究
发布时间：2018-06-29 21:05
本文选题：氧化应激 + 白细胞介素17 ； 参考：《重庆医科大学》2011年硕士论文
【摘要】：目的 研究细胞因子白细胞介素17(interleukine-17,IL-17)在Graves眼病(Graves' ophthalmopathy,GO)发病中的作用,并探讨氧化应激与IL-17的关系。 方法 25例初发活动期的Graves眼病病人作为GO组,15例健康人作为对照组纳入研究。临床评估GO病人眼病活动度(clinical activity score,CAS)及严重度(NOSPECS))。每位入选者均采集外周血,检测血清中游离三碘甲腺原氨酸(free triiodothyronine,FT3 )、游离四碘甲腺原氨酸(free tetraiodothyronine,FT4)、超敏促甲状腺激素(hypersensitive thyroid-stimulating hormone,μTSH)、甲状腺球蛋白抗体(thyroglobulin antibody,TgAb)、甲状腺过氧化物酶抗体(thyroid peroxidase antibody,TPOAb)、促甲状腺激素受体抗体(thyrotrophin receptor antibody ,TRAb)以及IL-17、丙二醛(monohydrate ,MDA)水平和超氧化物歧化酶(superoxide dismutase ,SOD)活力。 从对照组和GO组中各选取10例,采外周血。分离T淋巴细胞,体外与过氧化氢(Hydrogen Peroxide Solution,H2O2)和/维生素C(Vitamin C,Vitc)共同培养。每例标本的T淋巴细胞均分组为:空白组(H2O2 =0,VitC =0),过氧化氢组(H_2O_2 =1 000 nM,VitC=0),维生素C组(H_2O_2 =0,VitC =25μM),过氧化氢+维生素C组(H_2O_2 =1000 nM,VitC=25μM),检测细胞培养上清液IL-17水平,并进行统计分析。 结果 1.GO病人眼病活动度评分均≥3分(范围3-6,均值4.88±0.93),严重度NOSPECS≥3级(范围3-5,均值3.56±0.65)。 2.与对照组相比,GO组血清FT3(13.49±4.96 vs. 3.11±0.16 pg/ml),FT4(6.03±2.64 vs.1.24±1.36 ng/dl),TPOAb(300.38±233.05 vs.44.20±37.51 IU/ml),TgAb(1542.54±1614.51 vs.59.52±34.66 IU/ml),TRAb(19.41±3.77 vs.12.47±1.15U/L)及IL-17(13.62±2.43 vs. 10.15±0.93ng/L)水平较正常显著升高(P㩳0.01或P㩳0.05),μTSH (0.02±0.02 vs.1.24±0.14 mIU/L)显著降低(P㩳0.01),年龄无显著差异(30.00±5.30 vs.31.04±8.33岁)(P㧐0.05)。GO血清IL-17与眼病临床活动评分CAS(r=0.43,P=0.03)正相关,与严重度无相关。 3.GO组血清MDA较对照组(6.77±1.11 vs. 4.16±0.61nmol/ml)显著升高(P㩳0.05),SOD活力(53.95±17.53 vs. 73.16±15.33U/ml)显著降低(P㩳0.05)。GO组血清MDA水平与IL-17呈显著正相(r=0.490,P=0.046);MDA水平、SOD活力与眼病临床活动评分和严重度均无相关性(P㧐0.05)。 4 T淋巴细胞培养上清液IL-17水平检测分析,GO组中T淋巴细胞的H2O2组(27.55±4.75 ng/L)㧐空白组(20.24±2.13 ng/L )㧐VitC(18.76±1.13 ng/L)组及H2O2+VitC(17.59±1.85 ng/L)组(P㩳0.05);VitC组及H2O2+VitC组间无显著差异(P㧐0.05)。对照组的T淋巴细胞各组间无显著差异(P㧐0.05)。 结论 1.初发活动期GO病人外周血IL-17显著升高,并与CAS相关,可能参与了GO的发病; 2.氧化应激参与了GO病人IL-17水平的上调。VitC可在一定程度减少IL-17释放,为临床补充抗氧化剂作为GO辅助治疗提供思路和依据。
[Abstract]:Objective to investigate the role of interleukine-17 (IL-17) in the pathogenesis of Graves' ophthalmopathy (Graves' ophthalmopathy go) and to explore the relationship between oxidative stress and IL-17. Methods 25 patients with Graves' ophthalmopathy in the first active stage were included in the study as the go group and 15 healthy persons as the control group. Clinical evaluation of (clinical activity scorex and NOSPECS). In patients with go Each candidate collected peripheral blood, Serum free triiodothyronine (FT3), free tetraiodothyronine (FT4), hypersensitive thyroid-stimulating hormone (渭 TSH), thyroglobulin antibody-TgAb (TGAb), (thyroid peroxidase antibody TPOAb (TPOAb) and thyrotropin receptor (TPOAb) were detected. The levels of (thyrotrophin receptor antibody, IL-17, malondialdehyde (monohydrate) and the activity of superoxide dismutase (superoxide dismutase) were observed. Peripheral blood was collected from 10 cases in control group and 10 cases in go group. T lymphocytes were isolated and co-cultured with hydrogen peroxide (H _ 2O _ 2) and vitamin C (Vitamin C) in vitro. The T lymphocytes of each specimen were divided into three groups: blank group (H _ 2O _ 0 / Vit _ C _ (0), H _ 2O _ 2 / T _ 2 (H _ 2O _ 2 / 1 000 nM ~ (vit) C _ (10), Vitamin C _ (25 渭 M), H _ 2O _ 2O _ 2 / S (H _ 2O _ 2O _ 2 / M Vitamin C ~ (25 渭 M) and H _ 2O _ 2 / V _ 2O _ 2 vitamin C ~ (2 +) group (H _ 2O _ 2 / M ~ (2 000) N ~ (VitC) ~ (25 渭 M). Results 1. The scores of eye motion were 鈮，
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