Cox2、Cx26、Stathmin在喉乳头状瘤组织中的表达及临床意义

发布时间：2018-07-03 01:55

本文选题：喉乳头状瘤 + 免疫组织化学　；参考：《福建医科大学》2010年硕士论文

【摘要】： 目的通过检测Cox2、Cx26及Stathmin蛋白在喉乳头状瘤及声带息肉组织中的表达情况,探讨它们在喉乳头状瘤发生、发展过程中的作用及相互关系。方法采用免疫组织化学技术(PV-9000二步法)检测Cox2、Cx26及Stathmin蛋白在60例喉乳头状瘤和15例声带息肉组织中的表达情况,应用Image-ProPlus图像分析软件测定免疫组化图像的平均光密度(Mean Optical Density, MOD),运用SPSS 13.0软件进行统计学分析,观察各指标与喉乳头状瘤临床特征之间的关系及其在喉乳头状瘤组织中表达的相关性。结果(1)Cox2及Stathmin蛋白在喉乳头状瘤组织中的阳性表达率分别为73.33%、88.33%,均明显高于它们在声带息肉组织中的表达率(33.33%、53.33%),两者差异有统计学意义(P0.05)。(2)Cx26蛋白在喉乳头状瘤组织中的阳性表达率为33.33 %,明显低于其在声带息肉组织中的表达率(73.33 %),两者差异有统计学意义(P0.05)。喉乳头状瘤中Cx26蛋白的表达伴有细胞内定位的改变,Cx26蛋白在声带息肉组织中主要表达在细胞膜,而在喉乳头状瘤组织中的细胞膜、细胞浆和小部分细胞核中均有阳性表达。(3)Cox2、Cx26及Stathmin蛋白的表达水平与临床分型、是否复发、患者性别及年龄均无相关性(P0.05)。(4)喉乳头状瘤中,Cox2蛋白与Stathmin蛋白表达呈正相关(r=0.287,P0.05),而Cx26蛋白表达与Cox2及Stathmin蛋白表达呈负相关(r=-0.213、r=-0.184,P0.05)。结论 1. Cox2蛋白在喉乳头状瘤组织中高表达,提示其与喉乳头状瘤的发生可能有密切关系,有望为选择性Cox2抑制剂预防和治疗喉乳头状瘤提供依据。 2.喉乳头状瘤组织中Cx26蛋白的表达明显低于声带息肉,且伴有细胞内定位的改变,提示其在喉乳头状瘤的发生、发展过程可能起重要的作用。 3. Stathmin蛋白在喉乳头状瘤组织中高表达,提示其与喉乳头状瘤的多发性、浸润性可能有密切联系,可能成为研究生物治疗的方向之一。 4. Cox2蛋白与Stathmin蛋白在喉乳头状瘤组织中的表达呈正相关,提示它们可能在喉乳头状瘤的发生、发展过程中起协同作用。Cx26蛋白与Cox2蛋白及Stathmin蛋白在喉乳头状瘤组织中的表达均呈负相关,可能与它们之间的蛋白质相互作用有关,并可能与喉乳头状瘤的发生、发展有关。
[Abstract]:Objective to investigate the expression of Cox2Cx26 and Stathmin protein in laryngeal papilloma and vocal cord polyp, and to explore their roles in the development and development of laryngeal papilloma. Methods the expression of Cox2Cx26 and Stathmin protein in 60 cases of laryngeal papilloma and 15 cases of vocal cord polyps were detected by immunohistochemical technique (PV-9000 two-step method). The mean optical density (MOD) of immunohistochemical images was measured by Image-ProPlus image analysis software, and the statistical analysis was carried out by SPSS 13.0 software. To observe the relationship between the indexes and the clinical features of laryngeal papilloma and its expression in laryngeal papilloma. Results (1) the positive expression rates of Cox2 and stathmins in laryngeal papilloma were 73.33 and 88.33, respectively, which were significantly higher than those in vocal cord polyps (33.3353.33%). There was a significant difference between them (P0.05). (2) and Cx26 protein in laryngeal papilloma. The sexual expression rate was 33.33, which was significantly lower than that in vocal cord polyps (73.33%), the difference was statistically significant (P0.05). The expression of Cx26 protein in laryngeal papilloma is associated with the change of intracellular localization. Cx26 protein is mainly expressed in the cell membrane of vocal cord polyp, but in the cell membrane of laryngeal papilloma. (3) the expression level of Cox2Cx26 and Stathmin protein and clinical type, recurrence or not. There was no correlation between sex and age (P0.05). (4). There was a positive correlation between the expression of Cox2 protein and stathmin protein in laryngeal papilloma (P 0.05), but a negative correlation between the expression of Cx26 protein and the expression of Cox2 and stathmin protein (r-0.213r-0.184P0.05). Conclusion 1. The overexpression of Cox2 protein in laryngeal papilloma suggests that Cox2 protein may be closely related to the occurrence of laryngeal papilloma and may provide evidence for the prevention and treatment of laryngeal papilloma with selective Cox2 inhibitor. The expression of Cx26 protein in laryngeal papilloma was significantly lower than that in vocal cord polyp and accompanied by changes in intracellular localization, suggesting that Cx26 protein might play an important role in the occurrence and development of laryngeal papilloma. 3. The high expression of Stathmin protein in laryngeal papilloma suggests that Stathmin protein may be closely related to the multiple and invasive laryngeal papilloma and may be one of the research directions of biotherapy. Cox2 protein was positively correlated with the expression of stathmin protein in laryngeal papilloma, suggesting that Cox2 protein might occur in laryngeal papilloma. The expression of Cox2 protein and Stathmin protein in laryngeal papilloma was negatively correlated with the synergistic effect of Cx26 protein during the development of laryngeal papilloma, which may be related to the protein interaction between them, and to the occurrence and development of laryngeal papilloma.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.65

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