COX-2在声带息肉中的表达

发布时间：2018-07-03 00:54

本文选题：声带息肉 + COX-2　；参考：《昆明医学院》2011年硕士论文

【摘要】：[中文摘要]目的：检测环氧化酶-2(COX-2)在正常声带组织和声带息肉中的表达,了解COX-2对声带息肉患者发病的影响,进一步探讨声带息肉的发病机理,为临床防治提供理论依据。方法：选取经病理诊断确诊,且排除其他声带病变的声带息肉患者标本40例为实验组。取正常声带组织标本20例为对照组。所有标本经HE染色后,进行MaxVisionTM免疫组织化学染色,并行计算机图像定量分析。结果：在正常声带中,COX-2的表达较弱,而在声带息肉组织中的上皮细胞、炎性细胞、血管内皮细胞、腺体细胞、成纤维细胞表达较强。声带息肉组COX-2阳性单位均值(37.9950±2.15586)高于正常声带组(22.6235±3.20561),有统计学差异(p0.05)。结论：①声带息肉是多因素共同作用的结果,各种原因导致反复的声带损伤,引起局部充血、水肿、出血,粘膜上皮变性、坏死、脱落、增生,炎性细胞浸润,血管增生、扩张。②声带在长期的损伤过程中,由于COX-2与其它相关炎性介质的共同作用导致慢性炎症,血管内皮基底膜受损、血管壁通透性增强,一些本来不易通过血管壁的血管内物质进入组织间隙,导致声带粘膜固有层,特别是Reinke, s间隙水肿,炎性细胞浸润,纤维细胞增生,玻璃样变性等,固有层结缔组织排列紊乱,层状结构消失,息肉形成。③声带息肉发展过程中,合成COX-2促进息肉血管的形成,息肉血管增生,息肉获得丰富的血供从而不断生长。
[Abstract]:Objective: to detect the expression of cyclooxygenase-2 (COX-2) in normal vocal cord tissues and vocal cord polyps, to understand the effect of COX-2 on the pathogenesis of vocal cord polyps, and to provide a theoretical basis for clinical prevention and treatment. Methods: 40 patients with vocal cord polyps diagnosed by pathological diagnosis and excluded from other vocal cord lesions were selected as experimental group. 20 normal vocal cord specimens were taken as control group. All the specimens were stained with HE, then were stained by MaxVisionTM immunohistochemical staining and analyzed quantitatively by computer. Results: the expression of COX-2 was weak in normal vocal cords, but strong in epithelial cells, inflammatory cells, vascular endothelial cells, glandular cells and fibroblasts in vocal cord polyps. The mean value of COX-2 positive unit in vocal cord polyp group (37.9950 卤2.15586) was higher than that in normal vocal cord group (22.6235 卤3.20561) (p0.05). Conclusion 1 vocal cord polyp is the result of multiple factors. Repeated vocal cord injury, local hyperemia, edema, hemorrhage, mucosal epithelium degeneration, necrosis, exfoliation, proliferation, inflammatory cell infiltration and vascular hyperplasia are caused by various causes. During the long term injury of the vocal cord, the chronic inflammation caused by COX-2 and other related inflammatory mediators resulted in the injury of the endothelial basement membrane and the enhancement of the permeability of the vascular wall. Some substances that were not easy to pass through the vascular wall into the tissue space, leading to vocal cord mucosal lamina propria, especially Reinkes space edema, inflammatory cell infiltration, fibroblast proliferation, vitreous degeneration, lamina propria connective tissue disorder, In the process of polyp formation, COX-2 synthesis promotes polyp angiogenesis, polyp blood supply and growth.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R767.4

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