奥曲肽对氧诱导视网膜新生血管抑制作用的实验研究

发布时间：2018-07-03 07:55

本文选题：视网膜新生血管 + 奥曲肽　；参考：《山西医科大学》2010年硕士论文

【摘要】： 目的：在高氧诱导的视网膜新生血管模型的小鼠玻璃体腔注射奥曲肽(OA),观察奥曲肽对视网膜新生血管形成的抑制作用,及其抑制效果是否具有剂量依赖性。方法：将鼠龄7 d的小鼠40只随机分为正常组(N组)和高氧组(O组),高氧组分为对照组(O0组)和实验组(O1、02、03组)。正常组置于空气中,其他4组置于密闭氧箱中饲养5天后,返回正常环境饲养,正常组和高氧对照组玻璃体腔注射生理盐水3μl,实验组玻璃体腔注射不同剂量奥曲肽,分别为0.02μg、0.1μg、0.5μg各3μl。7天后处死小鼠,行视网膜铺片观察视网膜血管形态,组织切片观察并计数突破视网膜内界膜的血管内皮细胞核数目。结果： 1.视网膜血管形态观察：正常组(N组)视网膜血管均匀分布,管径较粗,分支良好,周边视网膜血管网结构清晰。高氧对照组(O0)可见视网膜血管分布不均匀,走行僵直,管径变细,视盘周围毛细血管闭塞,可见大片无灌注区,周边视网膜分支小血管狭窄,甚至闭塞,可见无灌注区。实验组(O1、O2、O3)随着奥曲肽剂量增加视网膜血管分布和形态逐渐改善,03组视网膜血管分布均匀,分支大致走行正常,周边视网膜血管网结构基本清晰。 2.视网膜新生血管计数：高倍镜(40×10)下可见每组平均每张切片突破视网膜内界膜的血管内皮细胞核数依次为(0.75±0.03)个/HP、(26.40±1.90)个/HP、(19.37±1.24)个/HP、(7.12±0.63)个/HP和(2.38±0.16)个/HP,N组和O0组比较差异有统计学意义(P0.05),O0、O1、02和03组间比较差异均具有统计学意义(P0.05)。结论：奥曲肽对高氧诱导的小鼠视网膜新生血管有抑制作用,且具有剂量依赖性。
[Abstract]:Aim: to observe the inhibitory effect of octreotide (OA) on retinal neovascularization in hyperoxia-induced retinal neovascularization model mice and whether the inhibitory effect is dose-dependent. Methods: forty mice aged 7 days were randomly divided into normal group (group N) and hyperoxia group (group O). Hyperoxia group was divided into control group (group O _ 0) and experimental group (group O _ 1o _ 2). Normal group was placed in air, the other four groups were fed in closed oxygen box for 5 days, then returned to normal environment to feed. Normal group and hyperoxia control group were injected with normal saline 3 渭 l into the vitreous cavity, and the experimental group were injected with different doses of octreotide in vitreous cavity. The mice were killed after 0. 02 渭 g / 0. 1 渭 g / g 0. 5 渭 g / g each 3 渭 l. 7 days later, the retinal vascular morphology was observed by retina preparation, and the number of vascular endothelial nuclei which broke through the inner retinal membrane was observed and counted by tissue section. Results: 1. Observation of retinal vascular morphology: normal group (group N) showed uniform distribution of retinal vessels, coarse diameter, good branches and clear structure of peripheral retinal vascular network. In the hyperoxia control group (O _ 0), the retinal vessels were distributed inhomogeneously, the retinal vessels were stiffened, the diameter became thinner, the capillaries around the optic disc were occluded, a large area without perfusion was seen, and the peripheral retinal branches were narrow, even occluded, and no perfusion area could be seen. With the increase of octreotide dose, the distribution and morphology of retinal vessels in the experimental group were gradually improved, the distribution of retinal vessels was uniform, the branches were normal, and the structure of peripheral retinal vascular network was clear. 2. Retinal neovascularization count: under high power microscope (40 脳 10), the average number of endothelial nuclei per section in each group was (0.75 卤0.03) HPs, (26.40 卤1.90) rHPs, (19.37 卤1.24) rHPs, (7.12 卤0.63) rHP and (2.38 卤0.16) rHPNs, respectively. Significant (P0.05) the differences between the two groups were statistically significant (P0.05). Conclusion: octreotide can inhibit retinal neovascularization induced by hyperoxia in a dose-dependent manner.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R774.1

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