氨基胍高选择性抑制iNOS在糖尿病大鼠视网膜中的研究

发布时间：2018-07-06 14:17

本文选题：糖尿病 + 氨基胍　；参考：《重庆医学》2014年19期

【摘要】：目的探讨氨基胍对糖尿病大鼠视网膜的治疗作用及机制。方法 60只大鼠分为对照组(n=20)、糖尿病组(n=20)、氨基胍治疗组(n=20),给药14d后取大鼠眼组织进行病理学观察(HE染色),采用ELISA、Western blot、PT-PCR检测各组大鼠诱导型一氧化氮合酶(iNOS)、内皮细胞性一氧化氮合酶(eNOS)、神经型一氧化氮合酶(nNOS)水平及表达的差异。结果HE染色结果显示,氨基胍治疗组与糖尿病组比较,大鼠视网膜组织缺损明显降低,神经元细胞增多。氨基胍治疗组、对照组iNOS水平显著低于糖尿病组,对照组nNOS水平明显低于其他两组;3组eNOS水平差异无统计学意义(P0.05)。氨基胍治疗组与糖尿病组比较,iNOS蛋白表达降低(P0.05),与对照组比较差异无统计学意义(P0.05)。氨基胍治疗组大鼠iNOS mRNA表达低于eNOS mRNA和nNOS mRNA。结论氨基胍可以改善糖尿病大鼠视网膜组织病变,作用途径可能是选择性地抑制iNOS的活性。
[Abstract]:Objective to investigate the therapeutic effect and mechanism of aminoguanidine on retina of diabetic rats. Methods Sixty rats were divided into three groups: control group (n = 20), diabetic group (n = 20) and aminoguanidine group (n = 20). After 14 days of administration, histopathology of rat eyes was observed by HE staining. Inducible nitric oxide synthase (iNOS) and endothelial cells were detected by ELISA-Western blotPT-PCR. The level and expression of nitric oxide synthase (Enos) and neuronal nitric oxide synthase (nNOS) were different. Results the results of HE staining showed that the retinal tissue defect was significantly decreased and the number of neuronal cells increased in the aminoguanidine treated group compared with the diabetic group. In aminoguanidine treatment group, the level of iNOS in control group was significantly lower than that in diabetic group, and the level of nNOS in control group was significantly lower than that in other two groups (P0.05). Aminoguanidine treatment group and diabetes group compared with the expression of iNOS protein decreased (P0.05), compared with the control group, there was no significant difference (P0.05). The expression of iNOS mRNA in aminoguanidine group was lower than that in Enos mRNA and nNOS mRNA. Conclusion Aminoguanidine can improve retinopathy in diabetic rats by selectively inhibiting iNOS activity.
【作者单位】：上海交通大学附属第一人民医院眼科;
【基金】：上海市自然科学基金资助项目(13ZR1433200) 上海市眼底病重点实验室开放课题基金资助项目(07Z22911)
【分类号】：R774.1;R587.2

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