左旋多巴甲酯对斜视性弱视猫模型的作用

发布时间：2018-07-06 16:25

本文选题：左旋多巴甲酯 + 斜视性弱视　；参考：《中国药理学通报》2010年01期

【摘要】：目的观察左旋多巴甲酯对斜视性弱视猫视皮质c-fos基因表达的影响,探讨盐酸左旋多巴甲酯对弱视的治疗作用及其作用机制。方法正常幼猫30只随机分成6组:左旋多巴甲酯低剂量、中剂量和高剂量组、模型对照组、阳性对照组及正常对照组,每组5只,于4wk时行眼外直肌切除术造成人工斜视(正常对照组除外),经图形视觉诱发电位(P-VEP)确定形成弱视后,灌胃给予左旋多巴甲酯20,40,80mg·kg-1,阳性对照组给予左旋多巴40mg·kg-1,正常对照组及模型组均给予等量生理盐水。观察P-VEP的变化并采用原位杂交技术检测各组猫视皮质的c-fosmRNA表达情况。结果左旋多巴甲酯明显的缩短猫斜视性弱视眼的P100波峰潜时及提高P100波幅值。斜视猫视皮质的阳性染色细胞较正常猫减少,差异有显著性(P0.01),用药后各组阳性染色细胞较模型对照组明显增加(P0.01)。结论左旋多巴甲酯明显地改善斜视性弱视猫模型弱视眼的传导和感觉功能,其机制可能与左旋多巴甲酯进入脑内的量增加及介导视皮质c-fos mRNA的表达调控有关。
[Abstract]:Objective to observe the effect of levodopa methyl ester on the expression of c-fos gene in the visual cortex of strabismic amblyopic cats and to explore the therapeutic effect and mechanism of L-dopa hydrochloride on amblyopia. Methods 30 normal kittens were randomly divided into 6 groups: low dose, middle dose and high dose group, model control group, positive control group and normal control group. Artificial strabismus was induced by extraocular rectus myectomy at 4wk (except for normal control group), and amblyopia was determined by pattern visual evoked potential (P-VEP). The normal control group and the model group were given the same amount of normal saline. The positive control group was given 40mg kg-1, and the normal control group and the model group were given the same amount of normal saline. The changes of P-VEP and the expression of c-fos mRNA in the visual cortex of the cats were detected by in situ hybridization. Results L-dopa methyl ester significantly shortened the peak time of P 100 wave and increased the amplitude of P 100 wave in the amblyopia eyes with strabismus. The number of positive staining cells in the visual cortex of strabismus cats was significantly lower than that in normal cats (P0.01), and the number of positive staining cells in each group was significantly higher than that in the model control group (P0.01). Conclusion L-dopa methyl ester can obviously improve the conduction and sensory function of amblyopia in strabismic amblyopia cat model. The mechanism may be related to the increase in the amount of L-dopa methyl ester entering into the brain and the regulation of c-fos mRNA expression in the visual cortex.
【作者单位】：广西医科大学药理学教研室;
【基金】：国家自然科学基金资助项目(No30760285) 广西研究生教育创新计划资助项目(No2008105981007D27)
【分类号】：R-332

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