喉鳞状细胞癌中Bak、Bcl-xl的表达及其与EB病毒的相关性研究

发布时间：2018-07-07 09:52

本文选题：凋亡 + Bak　；参考：《哈尔滨医科大学学报》2014年06期

【摘要】：目的检测凋亡相关基因Bak、Bcl-xl在喉鳞状细胞癌中的表达,探讨其与EB病毒感染的关系。方法采用原位杂交方法检测50例原发性喉鳞癌和20例癌旁正常喉黏膜的石蜡标本组织中EB病毒表达情况。免疫组织化学染色方法检测Bak和Bcl-xl蛋白的表达。结果喉鳞癌与癌旁正常喉黏膜中EB病毒表达情况存在显著差异(P0.01)。在喉鳞癌中Bak的阳性表达率为40.0%(20/50),Bcl-xl为50.0%(25/50)。18例EB病毒原位杂交阳性的喉鳞癌组织中Bak阳性检出3例,32例EB病毒原位杂交阴性的喉癌组织中Bak阳性检出17例,两组间存在显著差异(P0.05)。18例EB病毒原位杂交阳性的喉鳞癌组织中Bcl-xl阳性检出14例,32例EB病毒原位杂交阴性的喉癌组织中Bcl-xl阳性检出11例,两组间存在显著差异(P0.01)。结论喉癌组织中Bak、Bcl-xl基因表达的失调可能与喉癌的发生、发展有关;EB病毒在喉癌的发生机制中与Bak、Bcl-xl基因有协同作用。
[Abstract]:Objective to investigate the expression of apoptosis-related gene Bcl-xl in laryngeal squamous cell carcinoma (LSCC) and its relationship with Epstein-Barr virus (EBV) infection. Methods Epstein-Barr virus (EBV) expression in paraffin tissues of 50 cases of primary laryngeal squamous cell carcinoma and 20 cases of adjacent normal laryngeal mucosa was detected by in situ hybridization. The expression of Bak and Bcl-xl protein was detected by immunohistochemical staining. Results there was significant difference in EBV expression between laryngeal squamous cell carcinoma and adjacent normal laryngeal mucosa (P0.01). In laryngeal squamous cell carcinoma (LSCC), the positive rate of Bak was 40.0% (20 / 50) Bcl-xl was 50.0% (25 / 50). In 18 cases of laryngeal squamous cell carcinoma with EBV positive in situ hybridization, there were 3 cases of Bak positive and 17 cases of Bak positive in 32 cases of laryngeal carcinoma with EBV negative in situ hybridization. There was significant difference between the two groups (P0.05). Among 18 cases of laryngeal squamous cell carcinoma with EBV positive in situ hybridization, there were 14 cases of Bcl-xl positive and 11 cases of Bcl-xl positive in 32 cases of laryngeal carcinoma with EBV negative in situ hybridization. There was significant difference between the two groups (P0.01). Conclusion the imbalance of Bcl-xl gene expression in laryngeal carcinoma may be related to the occurrence of laryngeal carcinoma, and the development of EBV may play a synergistic role with Bcl-xl gene in the pathogenesis of laryngeal carcinoma.
【作者单位】：哈尔滨医科大学附属第二医院耳鼻咽喉-头颈外科;
【分类号】：R739.65

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