CD3、CD45RO在鼻咽癌中的表达及临床意义

发布时间：2018-07-16 12:04

【摘要】：【目的】探讨鼻咽癌中CD3、CD45RO的表达及其临床意义，为鼻咽癌的预后评估提供新的方法，及为其细胞免疫治疗提供理论依据。【方法】收集2012年1月至2013年1月间在苏州大学附属第一医院耳鼻咽喉科门诊喉镜下鼻咽部活检并经病理检查确诊为鼻咽癌的活检组织46例和非肿瘤性鼻咽部活检组织46例，所有肿瘤性和非肿瘤性标本均经10%甲醛固定，石蜡包埋切片，采用HE染色方法、免疫组织化学检测CD3和CD45RO的阳性表达水平。分析两种蛋白与鼻咽癌的临床病理特征之间的关系，以及两种蛋白相互之间的关系。【结果】（1）CD3在非肿瘤性鼻咽粘膜组织及鼻咽癌中的阳性表达率分别为69.57%、84.78%，在46例鼻咽癌病例中，CD3的表达水平与颈部淋巴结转移呈负相关（r=-0.484,P0.05），，与病理分化程度呈负相关（r=-0.33,P0.05），而与年龄、性别无明显相关性。（2）CD45RO在非肿瘤性鼻咽粘膜组织及鼻咽癌中的阳性表达率分别为91.30%、86.96%。在46例鼻咽癌病例中，CD45RO的表达水平与颈部淋巴结转移呈负相关（r=-0.294,P0.05），与性别亦有相关性（P0.05），与病理分化程度、年龄无明显相关性。（3）CD3和CD45RO表达之间有统计学意义（P0.05）。【结论】（1）CD3在鼻咽癌组织中的表达水平高于非肿瘤性鼻咽粘膜组织中的表达，CD45RO在鼻咽癌组织中的表达水平低于非肿瘤性鼻咽粘膜组织中的表达。（2）鼻咽癌组织的病理分化程度越低，CD3的阳性表达率越高。（3）CD3、CD45RO在鼻咽癌组织中高水平表达可能减少颈部淋巴结转移，CD3、CD45RO的表达水平较高者发生颈部淋巴结转移较少，而肿瘤的有无转移与其预后是密切相关的，从而推测此类患者预后相对较好。（4）CD3、CD45RO在抗瘤过程中可能有相关性。（5）与传统UICC—TNM组织学分级评估预后不同，通过对CD3、CD45RO的检测研究可以从另外一个层面评估鼻咽癌的预后情况，并为其细胞免疫治疗提供理论依据。
[Abstract]:[objective] to investigate the expression and clinical significance of CD3pCD45RO in nasopharyngeal carcinoma (NPC), and to provide a new method for prognosis evaluation of nasopharyngeal carcinoma (NPC). [methods] from January 2012 to January 2013, biopsy of nasopharynx was performed under laryngoscope and confirmed by pathological examination in the department of otolaryngology and otolaryngology, first affiliated Hospital of Suzhou University. 46 cases of nasopharyngeal carcinoma and 46 cases of non-neoplastic nasopharyngeal biopsy were studied. All tumor and non-tumor specimens were fixed with 10% formaldehyde and embedded in paraffin sections. The positive expression of CD3 and CD45RO was detected by HE staining and immunohistochemistry. To analyze the relationship between the two proteins and the clinicopathological features of nasopharyngeal carcinoma, [results] (1) the positive expression rates of CD3 in non-neoplastic nasopharyngeal mucosa and nasopharyngeal carcinoma were 69.57 and 84.78, respectively. In 46 cases of nasopharyngeal carcinoma, the expression level of CD3 and cervical lymph nodes were detected. There was a negative correlation between metastasis and pathological differentiation (P 0.05), but a negative correlation between metastasis and age (P 0.05). (2) the positive expression rates of CD45RO in nonneoplastic nasopharyngeal mucosa and nasopharyngeal carcinoma were 91.30 and 86.96, respectively. In 46 cases of nasopharyngeal carcinoma, the expression of CD45RO was negatively correlated with cervical lymph node metastasis (r-0.294), and correlated with sex (P0.05), and with the degree of pathological differentiation. (3) there was significant difference between the expression of CD3 and CD45RO (P0.05). [conclusion] (1) the expression of CD3 in nasopharyngeal carcinoma is higher than that in non-neoplastic nasopharyngeal mucosa. (2) the lower the pathological differentiation of nasopharyngeal carcinoma, the higher the positive expression rate of CD3. (3) the high level of CD3pCD45RO expression in nasopharyngeal carcinoma may reduce the expression of CD3nc-CD45RO in cervical lymph node metastasis. The cervical lymph node metastasis was less in the patients with higher level. However, metastasis is closely related to the prognosis of the tumor, which suggests that the prognosis of these patients is relatively good. (4) CD3+ CD45RO may be related to the anti-tumor process. (5) it is different from the traditional UICC-TNM histological grading in the evaluation of prognosis. Through the detection of CD3 and CD45RO, we can evaluate the prognosis of nasopharyngeal carcinoma on another level, and provide the theoretical basis for the cellular immunotherapy of nasopharyngeal carcinoma.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.63

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