COX-2、iNOS在原发性翼状胬肉中的表达及其相关研究

发布时间：2018-07-20 17:56

【摘要】：目的：①比较不同时期原发性翼状胬肉上皮细胞中环氧合酶-2(cyclooxygenase-2、COX-2)、诱导型一氧化氮合酶(induce-nitricoxide synthase、iNOS)的表达；②探讨环氧合酶2 (COX-2)和诱导型一氧化氮合酶(iNOS)对原发性翼状胬肉发生、发展的影响及其作用机制；探讨细胞凋亡在原发性翼状胬肉组织中的表达及其在发生发展过程中的作用。方法：选取2008年至2009年石河子大学第一附属医院眼科行手术切除的56例原发性翼状胬肉患者(56只眼)。按病变进行情况分为进行期和静止期,进行期40例(40只眼),静止期16例(16只眼)各组兼顾年龄、地区、种族及性别差异,所有患者排除眼部及身体其他部位疾病,排除其它眼科及全身疾病,其中男性19例,女性37例；年龄29～85岁,平均59岁；病程2-30年。正常结膜组织取自同时期行其他眼科手术并排除结膜疾病的20例患者术中所切除的少许正常结膜组织(均为手术中废弃不要的组织),男性12例,女性8例；年龄40-60岁,平均56岁。两组年龄比较,无显著性差异。取材前均经患者知情同意。全部标本均经石河子大学第一附属医院病理科统一切片确诊。病理标本做连续切片,厚3μm,分别进行HE染色和免疫组织化学Elivision染色。免疫组化结果判定综合染色强度及阳性细胞数两方面进行分析。同时采用脱氧核苷酸末端转移酶介导的脱氧尿苷三磷酸末端标记法(terminal deoxynucleotidyl transferase mediated 2'-deoxyuridine 5'-triphosphate-biotin nick end labeling, TUNEL),检测不同时期原发性翼状胬肉细胞中凋亡细胞的表达。结果：①COX-2在原发性翼状胬肉组织中表达上调,静止期、进行期与正常结膜之间比较差异有显著性意义,P0.001；静止期与正常结膜,进行期与正常结膜,以及静止期与进行期之间比较差异有意义,P0.05。iNOS在原发性翼状胬肉组织中表达上调,静止期、进行期与正常结膜之间比较差异有显著性意义,P0.001；进行期与正常结膜,以及静止期与进行期之间比较差异均有显著意义；静止期与正常结膜之间比较差异无意义,P0.05。②不同时期的原发性翼状胬肉组织中均发现凋亡细胞的表达。结论：一、COX-2和iNOS在原发性翼状胬肉发生发展中起一定的促进作用。二、COX-2和iNOS对原发性翼状胬肉发生发展起协同作用。三、原发性翼状胬肉中COX-2和iNOS引起的细胞凋亡与病程发展有一定关系。
[Abstract]:Objective to compare the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (induce-nitricoxide) in primary pterygium cells at different stages of primary pterygium. To investigate the role of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in the pathogenesis of primary pterygium. To investigate the expression of apoptosis in primary pterygium and its role in the development of pterygium. Methods: 56 patients (56 eyes) with primary pterygium were selected from 2008 to 2009 in the first affiliated Hospital of Shihezi University. There were 40 cases (40 eyes) in the progressive stage and 16 eyes (16 eyes) in the stationary stage according to the condition of the disease. All the patients were excluded from the eye and other parts of the body by taking into account the differences of age, region, race and sex. Other ophthalmic and systemic diseases were excluded, including 19 males and 37 females; age 2985 years, mean 59 years; course of disease 2-30 years. Normal conjunctival tissue was obtained from 20 patients who underwent other ophthalmic operations at the same time and excluded conjunctival diseases. They were 12 males and 8 females aged 40-60 years. The average age is 56. There was no significant difference in age between the two groups. Informed consent of the patient was obtained before the material was taken. All specimens were confirmed by unified section of Department of Pathology, first affiliated Hospital of Shihezi University. The pathological specimens were stained by HE staining and immunohistochemical Elivision staining respectively with 3 渭 m thick sections. The immunohistochemical results were used to determine the comprehensive staining intensity and the number of positive cells. The expression of apoptotic cells in primary pterygium cells at different stages was detected by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate end labeling (terminal deoxynucleotidyl transferase mediated 2'-deoxyuridine 5'-triphosphate-biotin nick end labeling, Tunel). Results the expression of COX-2 in primary pterygium was significantly higher than that in normal conjunctiva (P 0.001), the expression of COX-2 in primary pterygium was significantly higher than that in normal conjunctiva (P 0.001), the expression of COX-2 in primary pterygium was significantly higher than that in normal conjunctiva (P 0.001). The expression of P0.05.iNOS was up-regulated in primary pterygium tissue, and there was significant difference between resting stage, progressive stage and normal conjunctiva (P0.001). The expression of apoptotic cells was found in pterygium tissues at different stages of pterygium with no significant difference between resting phase and normal conjunctiva. Conclusion: COX-2 and iNOS play an important role in the development of primary pterygium. COX-2 and iNOS play a synergistic role in the development of primary pterygium. Thirdly, the apoptosis induced by COX-2 and iNOS in primary pterygium is related to the progression of pterygium.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R777.33

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