瘦素基因在甲状腺相关眼病眶脂肪及体外分化眶脂肪细胞中的表达

发布时间：2018-07-25 19:38

【摘要】：目的探讨瘦素基因在甲状腺相关眼病(thyroid associated ophthalmopathy,TAO)患者和正常人眼眶脂肪组织和体外诱导分化的眼眶脂肪细胞中的表达及意义。方法Trizol法提取8例(8眼)TAO患者和13例(13眼)正常人眼眶脂肪组织总RNA,采用实时荧光定量PCR检测瘦素mRNA的表达。体外培养TAO患者与正常人眼眶前脂肪成纤维细胞并进行诱导分化,实时荧光定量PCR检测分化后脂肪细胞中瘦素mRNA的表达。结果 TAO患者眼眶脂肪组织中瘦素mRNA的相对表达量为2.05±0.31(已排除年龄、性别和身体质量指数可能的影响),正常人眼眶脂肪组织中瘦素mRNA的相对表达量为0.69±0.24,两者相比差异有统计学意义(P0.05)。体外培养的TAO患者和正常人眼眶前脂肪成纤维细胞在诱导分化刺激下,均能分化为脂肪细胞;TAO患者分化第10天眼眶脂肪细胞的瘦素mRNA的相对表达量为1.55±0.44,正常人分化第10天眼眶脂肪细胞的瘦素mRNA的相对表达量为0.66±0.21,两者相比差异有统计学意义(P0.05)。结论瘦素基因在TAO患者眼眶脂肪组织和体外诱导分化的眼眶脂肪细胞中的表达均明显高于正常人,提示瘦素基因可能参与了TAO的发病机制。
[Abstract]:Objective to investigate the expression and significance of leptin gene in adipose tissue of (thyroid associated ophthalmopathy (thyroid associated ophthalmopathy TAO) and normal human orbital adipocytes induced by thyroid associated ophthalmopathy in vitro. Methods Total RNAs were extracted from 8 patients (8 eyes) of TAO and 13 cases (13 eyes) of normal controls by Trizol method. The expression of leptin mRNA was detected by real-time fluorescence quantitative PCR. Preorbital adipose fibroblasts from TAO patients and normal subjects were cultured in vitro and induced to differentiate. The expression of leptin mRNA in differentiated adipocytes was detected by real-time fluorescence quantitative PCR. Results the relative expression of leptin mRNA in orbital adipose tissue of TAO patients was 2.05 卤0.31 (excluded age). The relative expression of leptin mRNA in normal orbital adipose tissue was 0.69 卤0.24, the difference was statistically significant (P0.05). Cultured preorbital adipose fibroblasts from TAO patients and normal subjects were induced and stimulated by differentiation. The relative expression of leptin mRNA in orbital adipocytes was 1.55 卤0.44 on the 10th day of differentiation and 0.66 卤0.21 in normal persons on the 10th day after differentiation. There was significant difference between the two groups (P0.05). Conclusion the expression of leptin gene in orbital adipose tissue of TAO patients and orbital adipocytes induced by differentiation in vitro is significantly higher than that in normal controls, suggesting that leptin gene may be involved in the pathogenesis of TAO.
【作者单位】：南充爱尔麦格眼科医院;华西医院眼科;
【基金】：国家自然科学基金资助(编号:30371518)~~
【分类号】：R771.3

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