阻塞性睡眠呼吸暂停低通气综合征患者白细胞粘附分子CD15、CD11c的表达和ROS产生的研究
发布时间：2018-07-26 08:42
【摘要】： 目的：探讨OSAHS患者白细胞粘附分子CD15、CD11c的表达、ROS产生和它们之间的相关性,以及nCPAP治疗对粘附分子CD15、CD11c及其ROS水平的影响。 方法：按入院时间选择30例多导睡眠监测确诊的OSAHS患者作为实验组,男性28例,女性2例,年龄25-65岁(平均43.90±10.69岁),并将年龄和BMI匹配的12例健康志愿者作为对照组。所有参与者均签署知情同意书。以清晨空腹静脉血(5ml)作为血液标本,比较实验组和对照组单核细胞、淋巴细胞CD15、CD11c的表达、ROS产生和它们之间的差异和相关性,随机对实验组中的10例行nCPAP治疗,并分析nCPAP治疗前后各项指标的变化。 结果： 1.单核细胞和淋巴细胞粘附分子CD15、CD11c的表达。OSAHS组淋巴细胞CD15的表达高于正常组(t=3.048,P＜0.05)；OSAHS组淋巴细胞CD11c的表达高于正常组(t=2.658,P＜0.05)；OSAHS组单核细胞CD15的表达高于正常组(t=2.726,P＜0.05);OSAHS组单核细胞CD11c的表达高于正常组(t=1.611,P0.05)。 2.单核细胞中ROS产生的检测。OSAHS组单核细胞基础的ROS的产生高于正常组(t=7.859,P＜0.05),加入Rosup刺激物后,OSAHS组在单核细胞ROS的产生高于基础ROS的产生,而且高于对照组(t=12.038,P＜0.05) 3.淋巴细胞中ROS产生的检测。OSAHS组淋巴细胞基础ROS的产生高于正常组(t=16.250,P＜0.05),加入Rosup刺激物后,OSAHS组单核细胞ROS的产生高于基础ROS的产生,而且高于对照组(t=7.436,P＜0.05)。 4. nCPAP治疗的影响。nCPAP治疗后淋巴细胞CD15的表达较OSAHS组下调(t=4.257,P＜0.05),CD11c的表达较OSAHS组下调(t=3.997,P0.05),单核细胞CD15的表达较OSAHS组下调(t=2.593,P0.05),单核细胞CD11c的表达较OSAHS组下调(t=2.736,P＜0.05)。nCPAP治疗后淋巴细胞基础ROS的产生较OSAHS组下调(t=4.701,P0.05),单核细胞基础ROS的产生较OSAHS组下调(t=5.739,P＜0.05),Rosup刺激组淋巴细胞ROS的产生下调(t=6.008,P0.05)。Rosup刺激组单核细胞ROS的产生也下调(t=6.751,P0.05)。 5.ROS的产生与CD15、CD11c两种粘附分子的相关性。淋巴细胞中CD15与基础活性氧及Rosup刺激组是相关的(r=0.888,P＜0.01；r=0.799,P＜0.01),淋巴细胞中CD11c与基础活性氧及Rosup刺激组是相关的(r=0.901,P＜0.01；r=0。849,P＜0.01),单核细胞中CD15与基础活性氧及Rosup刺激组是相关的(r=0.766,P0.01；r=0.663,P0.01),单核细胞中CD11c与基础活性氧及Rosup刺激组是相关的(r=0.812,P0.01；r=0.667,P＜0.01)。 结论： 1. OSAHS患者淋巴细胞、单核细胞CD15、CD11c两种粘附分子的表达增强。 2. OSAHS患者淋巴细胞、单核细胞ROS的产生增强。 3. nCPAP治疗可以下调淋巴细胞、单核细胞粘附分子CD15、CD11的表达、减低ROS的产生。 4.ROS的产生与CD15、CD11c两种粘附分子的增强表达相关。
[Abstract]:Objective: to investigate the expression of CD15pCD11c in leukocyte of patients with OSAHS and its correlation with Ros, and to investigate the effect of nCPAP therapy on CD15 CD11c and its ROS level. Methods: 30 patients with OSAHS diagnosed by polysomnography were selected as experimental group (28 males and 2 females, aged 25-65 years, mean 43.90 卤10.69 years), and 12 healthy volunteers matched with BMI were used as control group. All participants sign informed consent. Early morning fasting venous blood (5ml) was used as blood sample to compare the expression of CD15 ~ + CD11c in monocytes and lymphocytes and the difference and correlation between them. 10 cases of experimental group were treated with nCPAP randomly. The changes of indexes before and after nCPAP treatment were analyzed. Results: 1. The expression of CD15 in lymphocytes in OSAHS group was higher than that in normal group (t 3.048g P < 0. 05). The expression of CD11c in OSAHS group was higher than that in normal group (t 2. 658% P < 0. 05). The expression of CD15 in monocytes in OSAHS group was higher than that in normal group (t = 2.726, P < 0. 05). The expression of CD11c in monocytes in OSAHS group was higher than that in normal group (t 1. 611). 2. Detection of ROS production in monocytes. The ROS production of monocytes in the OSAHS group was higher than that in the normal group (t = 7.859, P < 0. 05), and the production of ROS in monocytes was higher than that in the basic ROS group after the addition of Rosup stimulator, and was higher than that in the control group (t = 12. 038, P < 0. 05). Detection of ROS production in lymphocytes. The production of basic ROS in lymphocytes in OSAHS group was higher than that in normal group (t = 16.250, P < 0. 05). The ROS production of monocytes in OSAHS group was higher than that in basic ROS group after adding Rosup stimulator. The expression of CD11c in nCPAP group was lower than that in OSAHS group (t 4.257, P < 0. 05), the expression of CD15 in monocytes was lower than that in OSAHS group (P0.05), the expression of CD15 in monocytes was lower than that in OSAHS group (t2. 593P 0.05), and the expression of CD11c in monocytes was lower than that in OSAHS group (P < 0. 05), and the expression of CD15 in monocytes was lower than that in OSAHS group (P < 0. 05), and the expression of CD15 in monocytes was lower than that in OSAHS group (P < 0. 05). The expression of CD11c was lower than that of OSAHS group (tnCPAP). After treatment with nCPAP, the production of basic ROS in lymphocytes was lower than that in OSAHS group (p0.05), and the production of basic ROS in monocytes was lower than that in OSAHS group (t 5.739U P < 0. 05). The ROS production of lymphocytes in rosup-stimulated group was down-regulated (t6.008p0.05). The production of ROS was also down-regulated (P 0.05). The production of 5.ROS was correlated with CD15 and CD11c. CD15 in lymphocytes was correlated with basal reactive oxygen species (Ros) and Rosup stimulation group (P < 0.01), and CD11c in lymphocytes was correlated with basal Ros and Rosup (r 0.901U P < 0.01). CD15 in monocytes was correlated with basal reactive oxygen species (Ros) and Rosup stimulation (rP0. 766G, P 0. 01). CD11c in monocytes was correlated with basal reactive oxygen species (Ros) and Rosup (P < 0. 01). Conclusion: 1. The expression of CD15 and CD11c in lymphocytes and monocytes of OSAHS patients was increased. 2. 2. In patients with OSAHS, the production of monocyte ROS was increased. 3. The expression of monocyte adhesion molecule CD15, CD11, was down-regulated by nCPAP treatment. To reduce the production of ROS. The production of 4.ROS is related to the enhanced expression of CD15, CD11c and CD11c.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R766
本文编号：2145469
[Abstract]:Objective: to investigate the expression of CD15pCD11c in leukocyte of patients with OSAHS and its correlation with Ros, and to investigate the effect of nCPAP therapy on CD15 CD11c and its ROS level. Methods: 30 patients with OSAHS diagnosed by polysomnography were selected as experimental group (28 males and 2 females, aged 25-65 years, mean 43.90 卤10.69 years), and 12 healthy volunteers matched with BMI were used as control group. All participants sign informed consent. Early morning fasting venous blood (5ml) was used as blood sample to compare the expression of CD15 ~ + CD11c in monocytes and lymphocytes and the difference and correlation between them. 10 cases of experimental group were treated with nCPAP randomly. The changes of indexes before and after nCPAP treatment were analyzed. Results: 1. The expression of CD15 in lymphocytes in OSAHS group was higher than that in normal group (t 3.048g P < 0. 05). The expression of CD11c in OSAHS group was higher than that in normal group (t 2. 658% P < 0. 05). The expression of CD15 in monocytes in OSAHS group was higher than that in normal group (t = 2.726, P < 0. 05). The expression of CD11c in monocytes in OSAHS group was higher than that in normal group (t 1. 611). 2. Detection of ROS production in monocytes. The ROS production of monocytes in the OSAHS group was higher than that in the normal group (t = 7.859, P < 0. 05), and the production of ROS in monocytes was higher than that in the basic ROS group after the addition of Rosup stimulator, and was higher than that in the control group (t = 12. 038, P < 0. 05). Detection of ROS production in lymphocytes. The production of basic ROS in lymphocytes in OSAHS group was higher than that in normal group (t = 16.250, P < 0. 05). The ROS production of monocytes in OSAHS group was higher than that in basic ROS group after adding Rosup stimulator. The expression of CD11c in nCPAP group was lower than that in OSAHS group (t 4.257, P < 0. 05), the expression of CD15 in monocytes was lower than that in OSAHS group (P0.05), the expression of CD15 in monocytes was lower than that in OSAHS group (t2. 593P 0.05), and the expression of CD11c in monocytes was lower than that in OSAHS group (P < 0. 05), and the expression of CD15 in monocytes was lower than that in OSAHS group (P < 0. 05), and the expression of CD15 in monocytes was lower than that in OSAHS group (P < 0. 05). The expression of CD11c was lower than that of OSAHS group (tnCPAP). After treatment with nCPAP, the production of basic ROS in lymphocytes was lower than that in OSAHS group (p0.05), and the production of basic ROS in monocytes was lower than that in OSAHS group (t 5.739U P < 0. 05). The ROS production of lymphocytes in rosup-stimulated group was down-regulated (t6.008p0.05). The production of ROS was also down-regulated (P 0.05). The production of 5.ROS was correlated with CD15 and CD11c. CD15 in lymphocytes was correlated with basal reactive oxygen species (Ros) and Rosup stimulation group (P < 0.01), and CD11c in lymphocytes was correlated with basal Ros and Rosup (r 0.901U P < 0.01). CD15 in monocytes was correlated with basal reactive oxygen species (Ros) and Rosup stimulation (rP0. 766G, P 0. 01). CD11c in monocytes was correlated with basal reactive oxygen species (Ros) and Rosup (P < 0. 01). Conclusion: 1. The expression of CD15 and CD11c in lymphocytes and monocytes of OSAHS patients was increased. 2. 2. In patients with OSAHS, the production of monocyte ROS was increased. 3. The expression of monocyte adhesion molecule CD15, CD11, was down-regulated by nCPAP treatment. To reduce the production of ROS. The production of 4.ROS is related to the enhanced expression of CD15, CD11c and CD11c.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R766
【参考文献】
相关期刊论文 前1条
1 中华医学会呼吸病学分会睡眠呼吸疾病学组;阻塞性睡眠呼吸暂停低通气综合征诊治指南(草案)[J];中华结核和呼吸杂志;2002年04期
，本文编号：2145469
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