髓源性抑制细胞通过诱导上皮-间质转化促进鼻咽癌细胞的侵袭转移
发布时间：2018-07-27 11:37
【摘要】：目的研究髓源性抑制细胞(myeloid derived suppressor cells,MDSC)与鼻咽癌细胞上皮-间质转化(epithelialmesenchymal transition,EMT)的关系及对鼻咽癌细胞侵袭转移的影响。方法用流式细胞技术检测16例鼻咽癌患者以及9例健康对照者骨髓中MDSC所占比例,并分离鼻咽癌组织中MDSC;将MDSC与鼻咽癌细胞株CNE-2共培养,观察CNE-2细胞形态学的改变;应用q RT-PCR技术从RNA水平检测共培养前后CNE-2细胞上皮-间质转化相关基因(E-cadherin,N-cadherin,Vimentin,Snail)的改变;进一步采用Western blot技术从蛋白水平检测其表达变化;继而通过transwell小室及划痕实验检测MDSC对CNE-2细胞侵袭转移能力的影响。结果鼻咽癌患者骨髓中MDSC所占比例(10.37%)明显高于健康对照者(1.65%);MDSC与CNE-2细胞共培养后,CNE-2细胞在形态学上发生EMT;q RT-PCR和Western blot分别从RNA和蛋白水平证实CNE-2细胞上皮表型的相关基因(E-cadherin)表达下调,间质表型的相关基因(N-cadherin,Vimentin,Snail)表达上调;transwell小室侵袭实验证实共培养后CNE-2细胞的侵袭能力增强;划痕实验证实共培养后CNE-2细胞的迁移能力增强。结论 MDSC通过诱导EMT促进鼻咽癌细胞的侵袭转移能力。
[Abstract]:Objective to study the relationship between myeloid derived suppressor cells (MDSC) and epithelialmesenchymal transition (EMT) in nasopharyngeal carcinoma cells and the influence on the invasion and metastasis of nasopharyngeal carcinoma cells. Methods flow cytometry was used to detect the proportion of MDSC in 16 cases of nasopharyngeal carcinoma and 9 healthy controls. MDSC was isolated and isolated from nasopharyngeal carcinoma tissue. MDSC was co cultured with nasopharyngeal carcinoma cell line CNE-2 to observe the morphological changes of CNE-2 cells. The changes of CNE-2 cell epithelial mesenchymal transformation related genes (E-cadherin, N-cadherin, Vimentin, Snail) before and after co culture were detected by Q RT-PCR technology. The effect of MDSC on the invasion and metastasis of CNE-2 cells was detected by the Transwell chamber and scratch test. Results the proportion of MDSC in the bone marrow of the patients with nasopharyngeal carcinoma (10.37%) was significantly higher than that of the healthy controls (1.65%). After co culture of MDSC and CNE-2 cells, the CNE-2 cells were morphologically EMT; Q RT-PCR and Western. Blot confirmed that the expression of CNE-2 cell epithelial phenotype related genes (E-cadherin) was down regulated from RNA and protein levels, and the expression of interstitial phenotype related genes (N-cadherin, Vimentin, Snail) was up-regulated, and Transwell chamber invasion experiment confirmed that the invasion ability of CNE-2 cells increased after co culture, and the scratch test confirmed the migration energy of CNE-2 cells after co culture. Conclusion MDSC can promote the invasion and metastasis of nasopharyngeal carcinoma cells by inducing EMT.
【作者单位】： 解放军第324
【分类号】：R739.63
[Abstract]:Objective to study the relationship between myeloid derived suppressor cells (MDSC) and epithelialmesenchymal transition (EMT) in nasopharyngeal carcinoma cells and the influence on the invasion and metastasis of nasopharyngeal carcinoma cells. Methods flow cytometry was used to detect the proportion of MDSC in 16 cases of nasopharyngeal carcinoma and 9 healthy controls. MDSC was isolated and isolated from nasopharyngeal carcinoma tissue. MDSC was co cultured with nasopharyngeal carcinoma cell line CNE-2 to observe the morphological changes of CNE-2 cells. The changes of CNE-2 cell epithelial mesenchymal transformation related genes (E-cadherin, N-cadherin, Vimentin, Snail) before and after co culture were detected by Q RT-PCR technology. The effect of MDSC on the invasion and metastasis of CNE-2 cells was detected by the Transwell chamber and scratch test. Results the proportion of MDSC in the bone marrow of the patients with nasopharyngeal carcinoma (10.37%) was significantly higher than that of the healthy controls (1.65%). After co culture of MDSC and CNE-2 cells, the CNE-2 cells were morphologically EMT; Q RT-PCR and Western. Blot confirmed that the expression of CNE-2 cell epithelial phenotype related genes (E-cadherin) was down regulated from RNA and protein levels, and the expression of interstitial phenotype related genes (N-cadherin, Vimentin, Snail) was up-regulated, and Transwell chamber invasion experiment confirmed that the invasion ability of CNE-2 cells increased after co culture, and the scratch test confirmed the migration energy of CNE-2 cells after co culture. Conclusion MDSC can promote the invasion and metastasis of nasopharyngeal carcinoma cells by inducing EMT.
【作者单位】： 解放军第324
【分类号】：R739.63
【参考文献】
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1 马吉春;林江;姚冬明;邓兆群;钱炜;钱军;陈芹;杨静;李云;陈星星;闻向梅;郭z，
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