OPN基因多态性与广西壮族人群鼻咽癌易感性的关系

发布时间：2018-07-29 16:24

【摘要】：目的研究骨桥蛋白(osteopontin,OPN)基因单核苷酸多态性及其单倍型与广西壮族人群鼻咽癌(nasopharyngeal carcinoma,NPC)易感性的关系。方法采用病例-对照研究方法,收集2010-03-01-2013-03-20入住右江民族医学院附属医院的广西壮族鼻咽癌患者150例,同时随机选取2012-01-01-2012-08-01右江民族医学院附属医院常规体检的广西壮族人150名作为对照。采用单碱基延伸PCR技术和DNA测序法,检测150例广西壮族鼻咽癌患者和150名壮族对照者的OPN基因rs11728697和rs4754位点单核苷酸多态性,并分析OPN基因的单倍型频率。结果在OPN基因rs11728697位点上,携带CT基因型的个体相比携带常见的CC基因型个体罹患鼻咽癌的风险更高,OR=1.78,95%CI为1.05~2.98,χ2=5.863,P=0.033;但TT基因型并不增个体罹患鼻咽癌的风险,OR=0.92,95%CI为0.55~1.77,χ2=0.012,P=0.921。同时,鼻咽癌组在该位点的等位基因型频率与对照组的频率相比差异无统计学意义,OR=1.14,95%CI为0.69~1.45,χ2=0.545,P=0.466。在OPN基因rs4754位点上,对照组和鼻咽癌组的基因型及等位基因型频率分布差异无统计学意义,P0.05。对照组和鼻咽癌组的单倍型频率分布差异亦无统计学意义,P0.05。结论在广西壮族人群中,OPN基因rs11728697位点的CT基因型可能增加个体鼻咽癌的易感性,而rs4754位点的多态性与鼻咽癌的易感性无关。
[Abstract]:Objective to study the relationship between single nucleotide polymorphism and haplotype of osteopontin gene and susceptibility to nasopharyngeal carcinoma (NPC) in Guangxi Zhuang nationality. Methods A case-control study was conducted to collect 150 patients with nasopharyngeal carcinoma of Zhuang nationality in Guangxi, who were admitted to affiliated Hospital of Youjiang Medical College for nationalities from 2010-03-01-2013-03-20. At the same time, 150 Guangxi Zhuang people were randomly selected as the control group in the affiliated hospital of Youjiang Medical College for nationalities. The single nucleotide polymorphisms of OPN gene rs11728697 and rs4754 locus in 150 patients with nasopharyngeal carcinoma of Zhuang nationality and 150 controls of Zhuang nationality in Guangxi were detected by single base extension PCR technique and DNA sequencing. The haplotype frequency of OPN gene was analyzed. Results at the rs11728697 locus of OPN gene, individuals with CT genotype had a higher risk of developing nasopharyngeal carcinoma than those with common CC genotype, the CI was 1.75 卤2.98, 蠂 2 = 5.863, P 0.033, but TT genotype did not increase the risk of NPC. The CI of individuals with CT genotype was 0.551.77, and 蠂 2 0.012 with P + 0.921. At the same time, there was no significant difference in allelic frequency between nasopharyngeal carcinoma group and control group. At the rs4754 locus of OPN gene, there was no significant difference in genotype and allele frequencies between control group and nasopharyngeal carcinoma group (P 0.05). There was no significant difference in haplotype frequency distribution between control group and nasopharyngeal carcinoma group (P 0.05). Conclusion CT genotypes of rs11728697 locus in Guangxi Zhuang population may increase the susceptibility of NPC, but the polymorphism of rs4754 locus is not related to the susceptibility of NPC.
【作者单位】：右江民族医学院附属医院检验科;右江民族医学院中医药化学实验室;右江民族医学院附属医院桂西高发病防治实验中心;
【基金】：广西自然科学基金(2012GXSFBA053121) 百色市科学研究与技术开发项目(百科计20140617;20140901)
【分类号】：R739.63

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