αB-晶状体蛋白对大鼠急性高眼压后视网膜神经节细胞存活及凋亡蛋白Caspase-3表达的影响
发布时间：2018-07-29 16:23
【摘要】：目的 观察αB-晶状体蛋白对大鼠急性高眼压后视网膜神经节细胞存活及凋亡蛋白Caspase-3表达的影响。 方法 3月龄雄性Sprague-Dawley(SD)大鼠,体重250±20g ,160只完全随机设计分成以下4组:A组(急性高眼压组),B组(急性高眼压+玻璃体穿刺组),C组(急性高眼压+玻璃体注射生理盐水组),D组(急性高眼压+玻璃体注射αB-晶状体蛋白组),应用荧光金逆行标记计数法、免疫荧光法观察急性高眼压后各组大鼠视网膜神经节细胞在不同时间点的存活率及凋亡蛋白Caspase-3表达情况。 结果 急性高眼压后实验动物右眼(实验眼)视网膜神经节细胞开始减少,低倍荧光显微镜下可见全视网膜节细胞均在减少,第一周减少最为明显,两周后减少放缓。与其它3组相比,D组在急性高眼压后1周、2周、3周、4周视网膜神经节细胞存活率明显提高,差异具有统计学意义(P0.01),其它3组之间,差异无统计学意义(P0.05);急性高眼压后实验动物右眼(实验眼)Caspase-3免疫荧光染色出现阳性表达,与其它3组相比,D组在急性高眼压后24小时、48小时、72小时、1周的Caspase-3表达明显减少,差异具有统计学意义(P0.01),其它3组之间,差异无统计学意义(P0.05)。 结论 αB-晶状体蛋白对大鼠急性高眼压后视网膜神经节细胞存在保护作用,能增加视网膜神经节细胞的存活率。 αB-晶状体蛋白能抑制急性高眼压后视网膜神经节细胞内凋亡蛋白Caspase-3的表达,减少细胞凋亡。
[Abstract]:objective
Objective To observe the effect of alpha B- crystallin on the survival of retinal ganglion cells and the expression of apoptosis protein Caspase-3 after acute ocular hypertension in rats.
Method
3 month old male Sprague-Dawley (SD) rats, weighing 250 + 20g, were divided into 4 groups randomly: group A (acute high intraocular pressure group), group B (acute ocular hypertension + vitreous puncture group), group C (acute ocular hypertension + vitreous injection of saline group), group D (acute ocular hypertension + vitreous injection of alpha B- lens protein group), using fluorescein retrograde labeling The survival rate of retinal ganglion cells and the expression of apoptotic protein Caspase-3 in different groups of rat retinal ganglion cells after acute ocular hypertension were observed by the method of counting and immunofluorescence.
Result
The retinal ganglion cells in the right eye (experimental eye) of the experimental animals began to decrease after acute intraocular pressure. Under the low fluorescence microscope, the retinal ganglion cells were reduced, the first week decreased most obviously and decreased after two weeks. Compared with the other 3 groups, the survival rate of retinal ganglion cells in the D group was 1 weeks, 2 weeks, 3 weeks and 4 weeks after acute ocular hypertension. The difference was statistically significant (P0.01), the difference between the other 3 groups was not statistically significant (P0.05). The positive expression of Caspase-3 immunofluorescence staining in the right eye (experimental eye) after acute ocular hypertension was positive. Compared with the other 3 groups, the expression of Caspase-3 in the D group was significantly reduced at 24 hours, 48 hours, 72 hours and 1 weeks after acute ocular hypertension. The difference was statistically significant (P0.01), and there was no significant difference between the other 3 groups (P0.05).
conclusion
Alpha B- crystallin has protective effect on retinal ganglion cells after acute ocular hypertension in rats, and can increase the survival rate of retinal ganglion cells.
Alpha B- crystallin can inhibit the expression of apoptosis protein Caspase-3 and reduce apoptosis in retinal ganglion cells after acute ocular hypertension.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R775.9
[Abstract]:objective
Objective To observe the effect of alpha B- crystallin on the survival of retinal ganglion cells and the expression of apoptosis protein Caspase-3 after acute ocular hypertension in rats.
Method
3 month old male Sprague-Dawley (SD) rats, weighing 250 + 20g, were divided into 4 groups randomly: group A (acute high intraocular pressure group), group B (acute ocular hypertension + vitreous puncture group), group C (acute ocular hypertension + vitreous injection of saline group), group D (acute ocular hypertension + vitreous injection of alpha B- lens protein group), using fluorescein retrograde labeling The survival rate of retinal ganglion cells and the expression of apoptotic protein Caspase-3 in different groups of rat retinal ganglion cells after acute ocular hypertension were observed by the method of counting and immunofluorescence.
Result
The retinal ganglion cells in the right eye (experimental eye) of the experimental animals began to decrease after acute intraocular pressure. Under the low fluorescence microscope, the retinal ganglion cells were reduced, the first week decreased most obviously and decreased after two weeks. Compared with the other 3 groups, the survival rate of retinal ganglion cells in the D group was 1 weeks, 2 weeks, 3 weeks and 4 weeks after acute ocular hypertension. The difference was statistically significant (P0.01), the difference between the other 3 groups was not statistically significant (P0.05). The positive expression of Caspase-3 immunofluorescence staining in the right eye (experimental eye) after acute ocular hypertension was positive. Compared with the other 3 groups, the expression of Caspase-3 in the D group was significantly reduced at 24 hours, 48 hours, 72 hours and 1 weeks after acute ocular hypertension. The difference was statistically significant (P0.01), and there was no significant difference between the other 3 groups (P0.05).
conclusion
Alpha B- crystallin has protective effect on retinal ganglion cells after acute ocular hypertension in rats, and can increase the survival rate of retinal ganglion cells.
Alpha B- crystallin can inhibit the expression of apoptosis protein Caspase-3 and reduce apoptosis in retinal ganglion cells after acute ocular hypertension.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R775.9
【参考文献】
相关期刊论文 前6条
1 肖卫民,袁开宇,肖献忠,尢家，
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