CSMD1基因单核苷酸多态性与喉鳞状细胞癌的关系
发布时间：2018-08-04 11:03
【摘要】： 喉鳞状细胞癌(laryngeal squamous cell carcinoma,LSCC,简称喉癌),在我国东北地区发病率远高于其他地区,且近年来有明显增高的趋势,严重威胁着人类的健康与生命。结合地域优势,从肿瘤分子遗传学角度研究与喉癌发生、发展的相关遗传因素,寻找和定位喉癌相关基因,通过关联性研究,分析相关基因在不同人群中的关系,对从基因水平上揭示喉癌的发病机制有重要的意义。 实验目的:探讨吉林地区汉族CSMD1基因单核苷酸多态性(SNP)与喉鳞状细胞癌的关系。 实验方法:在CSMD1基因上选取位于第2内含子的rs13275581、第3内含子的rs7830462、第4内含子的rs12675316、第6内含子的rs17326768和第10内含子的rs17323393等五个SNP位点,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,配合2%琼脂糖凝胶电泳,对五个SNP位点与喉鳞状细胞癌的相关性进行统计学检验,探讨各个SNP位点与吉林地区汉族喉鳞状细胞癌发生的关系。 实验结果: 1喉癌组与正常对照组基因频率经Hardy-Weinberg平衡定律检验(P 0.05),说明实验所选人群与一般人群无显著性差异,具有群体代表性,可以代表吉林地区汉族人群。 2喉癌组与正常对照组之间,CSMD1基因SNP位点rs13275581的基因频率有显著性差异,提示CSMD1基因SNP位点rs13275581可能与吉林地区汉族人群喉癌发生相关。 3喉癌组与正常对照组之间,CSMD1基因rs7830462、rs12675316、rs17326768和rs17323393 SNP位点的基因频率差异均无统计学意义。CSMD1基因的这4个SNP位点与吉林地区汉族人群喉癌的发生未见关联性。
[Abstract]:The incidence of laryngeal squamous cell carcinoma (laryngeal squamous cell) in Northeast China is much higher than that in other regions, and in recent years, it is a serious threat to human health and life. Based on the regional advantage, the genetic factors related to the occurrence and development of laryngeal carcinoma were studied from the point of view of tumor molecular genetics, and the related genes of laryngeal cancer were searched and located, and the relationship between the related genes in different populations was analyzed. It is of great significance to reveal the pathogenesis of laryngeal carcinoma at the gene level. Objective: to investigate the relationship between single nucleotide polymorphisms (SNP) of CSMD1 gene and laryngeal squamous cell carcinoma (LSCC) in Jilin Han nationality. Methods: five SNP loci, rs13275581, rs7830462, rs12675316, intron 6 and intron 10, were selected from the CSMD1 gene, respectively, in intron 2, intron 3, intron 4, intron 6, and intron 10, respectively, and five SNP loci, rs13275581, rs7830462, rs12675316, intron 6 and intron 10, respectively, were selected. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with 2% agarose gel electrophoresis was used to detect the correlation between five SNP loci and laryngeal squamous cell carcinoma (LSCC). To investigate the relationship between SNP loci and laryngeal squamous cell carcinoma (LSCC) in Jilin Han nationality. Results: 1 the gene frequencies of laryngeal carcinoma group and normal control group were tested by Hardy-Weinberg equilibrium law (P0. 05). The results showed that there was no significant difference between the selected population and the general population. It can represent the Han population in Jilin region. 2 there was significant difference in the frequency of rs13275581 gene in SNP locus of CSMD1 gene between laryngeal carcinoma group and normal control group. The results suggest that the SNP rs13275581 of CSMD1 gene may be associated with laryngeal carcinoma in the Han nationality of Jilin region. 3 there is no significant difference in the frequency of rs7830462rs12675316rs17326768 and rs17323393 SNP locus between the laryngeal cancer group and the normal control group. The four SNP loci of CSMD1 gene were not associated with laryngeal cancer in Jilin Han population.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.65
本文编号：2163757
[Abstract]:The incidence of laryngeal squamous cell carcinoma (laryngeal squamous cell) in Northeast China is much higher than that in other regions, and in recent years, it is a serious threat to human health and life. Based on the regional advantage, the genetic factors related to the occurrence and development of laryngeal carcinoma were studied from the point of view of tumor molecular genetics, and the related genes of laryngeal cancer were searched and located, and the relationship between the related genes in different populations was analyzed. It is of great significance to reveal the pathogenesis of laryngeal carcinoma at the gene level. Objective: to investigate the relationship between single nucleotide polymorphisms (SNP) of CSMD1 gene and laryngeal squamous cell carcinoma (LSCC) in Jilin Han nationality. Methods: five SNP loci, rs13275581, rs7830462, rs12675316, intron 6 and intron 10, were selected from the CSMD1 gene, respectively, in intron 2, intron 3, intron 4, intron 6, and intron 10, respectively, and five SNP loci, rs13275581, rs7830462, rs12675316, intron 6 and intron 10, respectively, were selected. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with 2% agarose gel electrophoresis was used to detect the correlation between five SNP loci and laryngeal squamous cell carcinoma (LSCC). To investigate the relationship between SNP loci and laryngeal squamous cell carcinoma (LSCC) in Jilin Han nationality. Results: 1 the gene frequencies of laryngeal carcinoma group and normal control group were tested by Hardy-Weinberg equilibrium law (P0. 05). The results showed that there was no significant difference between the selected population and the general population. It can represent the Han population in Jilin region. 2 there was significant difference in the frequency of rs13275581 gene in SNP locus of CSMD1 gene between laryngeal carcinoma group and normal control group. The results suggest that the SNP rs13275581 of CSMD1 gene may be associated with laryngeal carcinoma in the Han nationality of Jilin region. 3 there is no significant difference in the frequency of rs7830462rs12675316rs17326768 and rs17323393 SNP locus between the laryngeal cancer group and the normal control group. The four SNP loci of CSMD1 gene were not associated with laryngeal cancer in Jilin Han population.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.65
【引证文献】
相关硕士学位论文 前1条
1 孙海波;CSMD1基因和BRG1基因与喉鳞状细胞癌发生的相关性研究[D];吉林大学;2012年
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