局部应用地塞米松对翼状胬肉组织中趋化因子受体CCR3表达的影响及意义

发布时间：2018-08-15 13:22

【摘要】：目的探讨术前局部应用地塞米松对趋化因子受体CCR3在翼状胬肉中表达的影响和意义。方法选取30例翼状胬肉患者,分为用药组和对照组,术前2周分别局部滴用3 g·L-1妥布霉素地塞米松眼液或3 g·L-1妥布霉素眼液,取手术切除的翼状胬肉组织;RT-PCR及Western blot法检测趋化因子受体CCR3 mRNA水平及蛋白水平的表达,进一步采用免疫组织化学法检测其蛋白水平的表达。结果翼状胬肉组织中CCR3在mRNA和蛋白水平均存在表达,但用药组CCR3 mRNA表达明显低于对照组,差异有统计学意义(t=-5.713,P=0.013),用药组CCR3的蛋白表达也明显低于对照组(t=-3.915,P=0.001)。免疫组织化学染色显示CCR3表达主要分布在结膜上皮细胞及部分血管内皮细胞上。结论趋化因子受体CCR3可能参与翼状胬肉的发生发展。地塞米松引起的CCR3表达改变是影响翼状胬肉预后、减少术后复发的可能机制之一。
[Abstract]:Objective to investigate the effect of dexamethasone on the expression of chemokine receptor CCR3 in pterygium before operation. Methods Thirty patients with pterygium were divided into two groups: drug group and control group. 3 g L-1 tobramycin dexamethasone eye solution or 3 g L tobramycin eye fluid were used 2 weeks before operation. The expression of chemokine receptor (CCR3 mRNA) and chemokine receptor protein (CCR3 mRNA) in pterygium were detected by RT-PCR and Western blot, and the expression of chemokine receptor protein was detected by immunohistochemistry. Results the expression of CCR3 in pterygium tissue was significantly lower than that in the control group (t ~ (-5.713) P ~ (0.013) and the expression of CCR3 protein in the treated group was also lower than that in the control group (t ~ (-3.915) P ~ (0. 001), but the expression of CCR3 mRNA in the treated group was significantly lower than that in the control group (t ~ (-5.713) P ~ (0. 01). Immunohistochemical staining showed that the expression of CCR3 was mainly in conjunctival epithelial cells and some vascular endothelial cells. Conclusion chemokine receptor CCR3 may be involved in the development of pterygium. The change of CCR3 expression induced by dexamethasone may affect the prognosis of pterygium and reduce the recurrence of pterygium.
【作者单位】：苏州大学附属第一医院眼科;苏州大学研究生院医学部;
【基金】：国家自然科学基金面上项目(编号:81200727,30972712) 江苏省医学重点人才项目(编号:RC2011104)~~
【分类号】：R777.33

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