鼓阶微孔注入盐酸椒苯酮胺对豚鼠庆大霉素耳蜗损伤的保护作用

发布时间：2018-08-29 20:07

【摘要】：目的观察盐酸椒苯酮胺(peperphentonamine hydrochloride,PPTA)对豚鼠庆大霉素(gentamicin,GM)耳蜗损伤的保护作用及机制。方法将听力正常的45只健康豚鼠随机分为空白对照组、GM组和PPTA组,每组15只。空白对照组行鼓阶开窗微孔注入人工外淋巴液10μl/d,连用3天;GM组给GM 160mg·kg-1·d-1肌肉注射,连用3天;PPTA组行鼓阶开窗微孔技术注入PPTA 10μl/d和肌肉注射GM 160mg·kg-1·d-1注,连用3天。分别于用药前和用药3天后对三组动物行听性脑干反应(auditory brainstem response,ABR)测试;第二次ABR测试后处死豚鼠,取出耳蜗,检测各组豚鼠耳蜗组织中过氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽(glutathione,GSH)含量,并以扫描电镜和透射电镜观察耳蜗细胞形态学改变。结果空白对照组、GM组、PPTA组ABR反应阈实验前比较差异无统计学意义,用药三天后分别为12.75±3.80、47.75±11.08、23.47±9.21dB SPL,各组间比较差异有统计学意义(P0.001);空白对照组、GM组、PPTA组SOD值比值分别为50.24±9.08、28.23±4.95、43.09±4.59U/mgprot,PPTA组SOD值显著高于GM组(P0.001);空白对照组、GM组、PPTA组GSH值分别为3.03±0.33、1.51±0.13、2.50±0.16Ggsh/L,PPTA组GSH值显著高于GM组(P0.001);扫描电镜观察PTTA组耳蜗各回外毛细胞肿胀、倒伏、缺失等较GM组轻,内毛细胞正常,而GM组内毛细胞亦有轻微损伤;透射电镜观察PPTA组耳蜗毛细胞肿胀、线粒体体聚集等改变明显轻于GM组。结论经鼓阶开窗微孔技术注入PPTA可通过清除GM产生的过量氧自由基从而发挥耳蜗保护作用。
[Abstract]:Objective to observe the protective effect and mechanism of peperphentonamine hydrochloride,PPTA on guinea pig gentamicin (gentamicin,GM) cochlea injury. Methods 45 healthy guinea pigs with normal hearing were randomly divided into control group GM group and PPTA group with 15 in each group. The control group was injected with artificial perilymph for 10 渭 l / d. GM 160mg kg-1 d-1 was injected intramuscularly in the GM group for 3 days, and PPTA 10 渭 l / d and GM 160mg kg-1 d-1 were injected intramuscularly for 3 days in the control group. The auditory brainstem response (auditory brainstem response,ABR) was measured before and 3 days after administration, the guinea pigs were killed after the second ABR test, the cochlea were removed, and the contents of superoxide dismutase,SOD and glutathione,GSH in cochlea of each group were detected. The morphological changes of cochlear cells were observed by scanning electron microscope and transmission electron microscope. Results there was no significant difference in the ABR reaction threshold before the test in the GM group in the blank control group. Three days after treatment, there were significant differences in SOD ratio between the three groups (12.75 卤3.80 卤11.08 卤23.47 卤9.21dB SPL, P 0.001), and the ratio of SOD value in the control group with GM was 50.24 卤9.08 卤4.95 卤4.95 渭 g / L, respectively, which was significantly higher than that in the GM group (P0.001), and the GSH value in the control group was 3.03 卤0.31.51 卤0.132.50 卤0.132.50 卤0.16GgshLPTA, respectively, which was significantly higher than that in the GM group (P0.001), and the GSH value in the control group was 3.03 卤0.31.51 卤0.132.50 卤0.132.50 卤0.16GgshLPTA, respectively, which was significantly higher than that in the GM group (P0.001). The outer hair cells of the cochlea in the PTTA group were observed to be swollen by scanning electron microscope. The hair cells in the GM group were slightly damaged, and the hair cells in the cochlea of the PPTA group were swollen and the mitochondrial body aggregation was significantly lighter than that in the GM group. Conclusion injection of PPTA via tympanic fenestration can protect cochlea by scavenging excessive oxygen free radicals produced by GM.
【作者单位】：南方医科大学珠江医院耳鼻咽喉-头颈外科;
【基金】：广东省科技计划项目(2008B030301150) 广东省教育部产学研结合项目(2010B090400421)资助
【分类号】：R764

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