Annexin1在喉鳞癌及癌旁组织的表达及临床意义

发布时间：2018-10-10 12:22

【摘要】： 目的：通过检测抑癌基因Annexin 1在喉鳞状细胞癌(LSCC)及癌旁不同距离组织中的表达情况,从蛋白水平及基因水平来探讨其与喉癌手术安全切缘的关系,并分析与临床分型、临床分期、病理分级和淋巴结转移的关系。方法：采用病理学观察、免疫组织化学法和RT-PCR法检测32例新鲜喉鳞癌标本的癌原发灶区与癌旁黏膜交界处0.2cm、0.5cm、1.0cm、2.0cm组织中Annexin 1的表达情况。结果：Annexin 1 (ANXAl)在距离喉鳞癌原发灶0.2cm→2.0cm中的表达强度依次递增；在癌旁0.5cm以外组织中,Annexin 1蛋白的染色强度很深,而在喉鳞癌原发灶及癌旁0.2cm组织中,Annexin 1蛋白的染色强度相对较低,两者之间统计学差异有显著性(P0.01)。免疫组化技术分析Annexin 1的表达与临床分期(P0.05)、病理分级(P0.05)均有关；与临床分型、淋巴结转移均无显著性差异(P0.05); RT-PCR法测定Annexin lmRNA的表达与喉鳞癌的临床分期(P0.05)、病理分级(P0.05)有关；与临床分型、淋巴结转移均无关(P0.05)。结论：Annexin 1蛋白及1mRNA在LSCC组织中表达量由原发灶到无癌区的递增性变化及癌旁0.5cm内外表达量的显著性差异表达,提示：Annexin 1可以作为LSCC恶性程度的分子生物学指标,指导临床病理分级及分期；癌旁0.5cm做为手术切缘是安全的。
[Abstract]:Objective: to investigate the expression of tumor suppressor gene Annexin 1 in laryngeal squamous cell carcinoma (LSCC) (LSCC) and its adjacent tissues at different distances from the level of protein and gene, and to analyze the relationship between the expression of Annexin 1 gene and the safe margin of laryngeal carcinoma operation, and to analyze the relationship between the expression and clinical type of laryngeal squamous cell carcinoma (LSCC). The relationship between clinical stage, pathological grade and lymph node metastasis. Methods: the expression of Annexin 1 in 32 cases of fresh laryngeal squamous cell carcinoma (LSCC) was detected by pathological observation, immunohistochemical method and RT-PCR method. Results: the expression intensity of Annexin 1 (ANXAl) in 0.2cm 2.0cm was increased in turn, and the staining intensity of Annexin 1 protein was very strong in the adjacent 0.5cm tissues of laryngeal squamous cell carcinoma (LSCC). In laryngeal squamous cell carcinoma (LSCC) and adjacent 0.2cm tissues, the staining intensity of Annexin 1 protein was relatively low, there was significant difference between them (P0.01). The expression of Annexin 1 was correlated with clinical stage (P0.05) and pathological grade (P0.05), and had no significant difference with clinical type and lymph node metastasis (P0.05). The expression of Annexin lmRNA was correlated with the clinical stage (P0.05) and pathological grade (P0.05) of laryngeal squamous cell carcinoma (P0.05). There was no correlation with clinical type and lymph node metastasis (P0.05). Conclusion: the expression of Annexin 1 protein and 1mRNA in LSCC tissues is increased from primary tumor to non-cancerous area, and there is significant difference in the expression of 0.5cm in and out of LSCC, suggesting that Annexin 1 can be used as a molecular biological index of the malignant degree of LSCC. It is safe to use the paracancerous 0.5cm as the surgical margin to guide the clinicopathological grading and staging.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.65

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