CXCR3、IP-10对高糖诱导的人脐静脉内皮细胞凋亡的影响

发布时间：2018-11-03 17:44

【摘要】：目的研究CXCR3及IP-10对高糖诱导的人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)凋亡的影响,并对其机制进行初步探讨。方法 HUVEC在高糖和正常糖DMEM培养液中分别培养24 h、48 h、72 h,流式细胞术检测凋亡率,Western blot检测CXCR3表达。反义肽核酸(asPNA)阻断高糖组细胞表达CXCR3后,Western blot检测CXCR3表达,流式细胞术检测凋亡率。CXCR3配体IP-10及拮抗剂AMG487干预高糖组细胞48 h后,流式细胞术检测凋亡率。结果高糖组48 h、72 h时细胞凋亡率(22.56±1.83)%、(25.33±2.34)%较对照组(3.01±0.27)%、(3.80±0.32)%增加显著(均为P0.01)。高糖组48 h、72 h CXCR3表达量0.57±0.04、0.87±0.03较对照组48 h 0.36±0.02、0.71±0.02明显上调(P0.01、0.05)。1μmol·L-1asPNA CXCR3组、2μmol·L-1asPNA CXCR3组CXCR3表达量0.32±0.02、0.14±0.02均较空白对照组(0.61±0.02)及阴性对照组(0.59±0.03)显著下降(均为P0.01)。同时,1μmol·L-1asPNA CXCR3组、2μmol·L-1asPNA CXCR3组细胞凋亡率(15.33±1.05)%、(9.00±0.76)%也较空白对照组(22.98±1.92)%及阴性对照组(21.34±1.73)%明显下降(均为P0.01)。IP-10组细胞凋亡率(38.05±2.88)%较高糖对照组(21.11±2.02)%、IP-10+AMG487组(20.80±2.17)%及AMG487组(19.54±1.93)%显著增加(均为P0.01)。结论 CXCR3、IP-10促进高糖诱导的HUVEC凋亡,其促凋亡机制与IP-10/CXCR3轴有关。
[Abstract]:Objective to investigate the effects of CXCR3 and IP-10 on the apoptosis of human umbilical vein endothelial cells (human umbilical vein endothelial cells,HUVEC) induced by high glucose, and to explore its mechanism. Methods HUVEC was cultured in high glucose and normal glucose DMEM medium for 24 h and 48 h for 72 h respectively. Apoptosis rate was detected by flow cytometry and CXCR3 expression was detected by, Western blot. The expression of CXCR3 was detected by, Western blot after blocking the expression of CXCR3 by antisense peptide nucleic acid (asPNA), the apoptosis rate was detected by flow cytometry, and the apoptosis rate was detected by flow cytometry after the intervention of CXCR3 ligand IP-10 and antagonist AMG487 for 48 h. Results the apoptosis rate was (22.56 卤1.83)%, (25.33 卤2.34)% at 48 h and (3.01 卤0.27)%, (3.80 卤0.32)% in high glucose group. The expression of CXCR3 in high glucose group (0.57 卤0.04) at 72 h was significantly higher than that in control group (48 h, 0.36 卤0.02 卤0.71 卤0.02). The expression of CXCR3 in 2 渭 mol L-1asPNA CXCR3 group was significantly lower than that in the blank control group (0.61 卤0.02) and the negative control group (0.59 卤0.03) (both P0.01). At the same time, the apoptosis rate of 1 渭 mol L-1asPNA CXCR3 group and 2 渭 mol L-1asPNA CXCR3 group was (15.33 卤1.05)%. (9.00 卤0.76)%, (22.98 卤1.92)% and (21.34 卤1.73)%, respectively. The apoptosis rate of IP-10 group was (38.05 卤2.88)% higher than that of control group (P 0.01). The exposure group was (21.11 卤2.02)%, IP-10 AMG487 group (20.80 卤2.17)% and AMG487 group (19.54 卤1.93)% increased significantly (P0.01). Conclusion CXCR3,IP-10 can promote the apoptosis of HUVEC induced by high glucose, and its mechanism is related to the IP-10/CXCR3 axis.
【作者单位】：上海交通大学医学院附属第三人民医院;上海交通大学医学院附属第一人民医院;
【基金】：上海市科委自然科学基金资助(编号:10ZR1418500) 上海市重点学科建设资助项目(编号:S30205)~~
【分类号】：R774.1

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