糖尿病大鼠角膜上皮损伤修复过程中occludin的重塑特点

发布时间：2018-11-06 11:18

【摘要】：目的探讨糖尿病(DM)大鼠和正常大鼠角膜上皮修复过程中紧密连接蛋白occludin重塑的特点。方法 90只SD大鼠随机分为DM组和正常对照组(NC)各45只(DM组均高脂喂养),通过腹腔注射链脲佐菌素(STZ)诱导形成Ⅱ型DM大鼠模型,于损伤后0 h(未损伤),16、48、72、120 h(每组5只)处死大鼠取角膜。间接免疫荧光染色和Western blot法观察occludin蛋白在损伤修复过程中的变化。结果DM大鼠角膜上皮损伤愈合率较NC组大鼠明显延迟,DM组和NC组大鼠角膜上皮occludin蛋白在损伤后16、48 h表达差异有统计学意义(P0.05),在损伤后0、72、120 h表达差异无统计学意义。结论糖尿病可影响大鼠角膜上皮创伤修复过程中紧密连接蛋白occludin蛋白的表达。
[Abstract]:Objective to investigate the characteristics of tight junction protein (occludin) remodeling during corneal epithelial repair in diabetic (DM) rats and normal rats. Methods 90 SD rats were randomly divided into DM group and normal control group (45 (NC) rats in each group). The model of type 鈪，

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