阿魏酸对视网膜色素变性小鼠血浆内皮素-1表达的影响

发布时间：2018-11-09 10:11

【摘要】：目的研究不同浓度阿魏酸作用下,出生后不同时期的视网膜色素变性(retinitis pigmentosa,RP)动物模型(rd小鼠)血浆中内皮素-1(endothelin-1,ET-1)动态表达特征,探讨阿魏酸对rd小鼠表达ET-1的作用特点。方法取新出生的rd小鼠90只及C57/BL6小鼠18只,按照阿魏酸灌胃浓度的不同分成4组,0.25 g·L-1组、0.50 g·L-1组、0.75 g·L-1组、1.00 g·L-1组,每组再按照给药时间分为2周组、3周组、4周组,以上12组为药物处理组,每组各6只。相同周龄的rd小鼠各6只不作处理作为病变对照组,相同周龄的C57/BL6小鼠各6只作为正常对照组。ELISA法检测血浆ET-1浓度。结果 2周组、3周组、4周组病变对照组小鼠ET-1浓度均高于正常对照组,尤其3周组、4周组与正常对照组相比差异有统计学意义(P=0.024、0.010)。经阿魏酸治疗后2周时,0.50 g·L-1组、0.75 g·L-1组ET-1水平低于病变对照组,但差异无统计学意义;3周时,0.25 g·L-1组、0.50 g·L-1组、0.75 g·L-1组ET-1水平低于病变对照组,其中0.50 g·L-1组差异有统计学意义(P=0.034);4周时,0.25 g·L-1组、0.50 g·L-1组、0.75 g·L-1组ET-1水平低于病变对照组,差异均有统计学意义(P=0.011、0.027、0.021)。各浓度治疗组中,仅1.00 g·L-1组治疗3周和4周时与正常对照组相比差异有统计学意义(P=0.013、0.009)。结论 rd小鼠在病变过程中,血浆ET-1水平明显升高,可能与RP的发生发展有关。在不同浓度阿魏酸治疗下,rd小鼠ET-1水平不同程度下降,其中0.50 g·L-1、0.75 g·L-1治疗效果最明显。
[Abstract]:Objective to study the dynamic expression of endothelin-1 (endothelin-1,ET-1) in plasma of different concentrations of ferulic acid at different postnatal stages of retinitis pigmentosa (retinitis pigmentosa,RP) animal model (rd mice). To investigate the effect of ferulic acid on the expression of ET-1 in rd mice. Methods 90 newly born rd mice and 18 C57/BL6 mice were divided into 4 groups according to the concentration of ferulic acid: 0.25 g L-1 group, 0.50 g L-1 group, 0.75 g L-1 group. 1.00 g L-1 group, each group was divided into 2 week group, 3 week group and 4 week group according to the time of administration. The above 12 groups were drug treatment group with 6 rats in each group. Six rd mice of the same age were untreated as control group and six C57/BL6 mice of the same age were used as normal control group. The plasma ET-1 concentration was detected by ELISA method. Results the levels of ET-1 in the 2 week group, 3 week group and 4 week group were higher than those in the normal control group, especially in the 3 week group. The difference between the 4 week group and the normal control group was statistically significant (P 0. 024 0. 010). At 2 weeks after ferulic acid treatment, the levels of ET-1 in 0.50 g L-1 group and 0.75 g L-1 group were lower than those in the control group, but the difference was not statistically significant. At 3 weeks, the levels of ET-1 in 0.25 g L-1, 0.50 g L-1 and 0.75 g L-1 groups were lower than those in the lesion control group, and there was a significant difference between 0.50 g L-1 group and 0.75 g L-1 group (P0. 034). At 4 weeks, the levels of ET-1 in 0.25 g L-1 group, 0.50 g L-1 group and 0.75 g L-1 group were significantly lower than those in the control group (P 0.011 0. 027 卤0. 021). Only 1.00 g L-1 group was significantly different from the control group at 3 and 4 weeks after treatment (P0. 013 ~ 0. 009). Conclusion the level of plasma ET-1 in rd mice increased significantly during the course of the lesion, which may be related to the occurrence and development of RP. Under different concentrations of ferulic acid, the level of ET-1 in rd mice decreased to different degrees, of which 0.50 g / L ~ (-1) and 0.75 g ~ (-1) g 路L ~ (-1) were the most effective.
【作者单位】：北京市首都医科大学附属北京同仁医院北京同仁眼科中心
【基金】：国家自然科学基金资助(编号:81271046) 北京市教委科研计划重点项目(编号:KZ201510025025)~~
【分类号】：R774.1

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