米诺环素对高眼压模型视网膜功能的影响
发布时间：2018-11-13 20:53
【摘要】： 目的：探讨米诺环素(minocycline)对兔慢性高眼压引起的视网膜功能的改变,为该药物在国内应用于眼科临床治疗视神经损害性疾病提供实验研究依据。 方法：成年健康色素兔30只,随机分为A、B、C三组,每组各10只。A组为空白对照组,B组为模型组,C组为米诺环素给药组。选B、C组右眼作高眼压模型,每眼抽取房水0.1ml,随后注射0.3%复方卡波姆溶液0.1ml；C组给予米诺环素口服给药,50mg*kg-1*d-1,首次加倍,连续30天。用Schiotz眼压计测量并记录眼压,一周一次。30天时,A、B、C组右眼均行光学相干眼底断层扫描(OCT)、闪光全视野视网膜电图(Flash electroretinogram, FERG)及闪光视诱发电位(Flash visual evoked potential, FVEP)检查,通过光学相干眼底断层扫描(OCT)检测视神经纤维层厚度,通过FERG观察比较a波、b波振幅变化情况；通过FVEP观察N1、P1、N2波峰时值的后延程度。最后用OLYMPUS光学显微镜在10×40倍镜下观察视网膜各层病理改变。SPSS13.0统计学分析软件对结果进行统计学分析。 结果：眼压值：在整个实验期间B、C组动物的眼压均高于22mmHg,最高达55mmHg,且在造模后各个时间点均高于A组。OCT所检测视神经纤维层厚度测量结果统计分析显示：视神经纤维层厚度B组最薄,C组次之,A组最厚,差异均具有统计学意义(P0.05)；FERG所检测结果统计分析显示：a波振幅B、C组较A组无明显下降,三组比较差异均无统计学意义(P0.05)；b波振幅B、C组较A组均有下降,且B组较C组下降明显,差异均具有统计学意义(P0.05); FVEP所检测各波结果统计分析显示：三个波的峰时值B、C组较A组均有延迟,且B组较C组延迟明显,差异均具有统计学意义(P0.05)；光镜下B组视网膜组织各层结构最紊乱,A组视网膜组织各层结构最完整,与A组比较,C组视网膜组织各层结构改变,但较B组改变轻微。 结论：米诺环素对兔慢性高眼压引起的视网膜损伤有保护作用。
[Abstract]:Objective: to investigate the effects of minocycline (minocycline) on retinal function induced by chronic intraocular pressure (IOP) in rabbits, and to provide experimental evidence for the clinical application of minocycline in the treatment of optic nerve injurious diseases in China. Methods: thirty adult healthy pigmented rabbits were randomly divided into three groups: group A (n = 10), group B (model group) and group C (n = 10) treated with minocycline. The right eyes of group B were selected as the model of high intraocular pressure, 0.1 ml aqueous humor was extracted from each eye, and then 0.1 ml of compound carbomer solution was injected into group C for oral administration of minocycline, 50 mg 路kg ~ (-1) 路d ~ (-1), doubling for the first time for 30 days. Intraocular pressure (IOP) was measured and recorded by Schiotz intraocular pressure meter. At 30 days a week, all right eyes in group C underwent optical coherence tomography (OCT),) flash pan-field electroretinogram (Flash electroretinogram, FERG) and flash visual evoked potential (Flash visual evoked potential,). FVEP), optical coherence tomography (OCT) was used to detect the thickness of optic nerve fiber layer, and the amplitude of a wave and b wave were observed and compared by FERG. The degree of delay of N _ 1 P _ 1N _ 2 peak was observed by FVEP. Finally, the pathological changes of various layers of retina were observed with OLYMPUS optical microscope under 10 脳 40 times microscope. The results were statistically analyzed by SPSS13.0 statistical analysis software. Results: intraocular pressure (IOP): the intraocular pressure of group B C was higher than 22 mm Hg, and the highest was 55 mm Hg during the whole experiment. Statistical analysis of the thickness of optic nerve fiber layer measured by OCT showed that the thickness of optic nerve fiber layer was the thinnest in group B, followed by group C, and thickest in group A. The difference was statistically significant (P0.05). The statistical analysis of FERG results showed that the amplitude of a wave in group C was not significantly lower than that in group A, and there was no significant difference among the three groups (P0.05). The amplitude of B wave in group B was significantly lower than that in group A (P0.05), and that in group B was significantly lower than that in group C (P0.05). Statistical analysis of the results of each wave detected by FVEP showed that the peak time of three waves in group C was delayed than that in group A, and the delay in group B was significantly higher than that in group C (P0.05). Under light microscope, the structure of each layer of retina in group B was the most disordered, and that in group A was the most complete. Compared with group A, the structure of each layer of retina in group C changed slightly, but it was slightly different from that in group B. Conclusion: minocycline has protective effect on retinal injury induced by chronic intraocular pressure in rabbits.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R775.1
本文编号：2330388
[Abstract]:Objective: to investigate the effects of minocycline (minocycline) on retinal function induced by chronic intraocular pressure (IOP) in rabbits, and to provide experimental evidence for the clinical application of minocycline in the treatment of optic nerve injurious diseases in China. Methods: thirty adult healthy pigmented rabbits were randomly divided into three groups: group A (n = 10), group B (model group) and group C (n = 10) treated with minocycline. The right eyes of group B were selected as the model of high intraocular pressure, 0.1 ml aqueous humor was extracted from each eye, and then 0.1 ml of compound carbomer solution was injected into group C for oral administration of minocycline, 50 mg 路kg ~ (-1) 路d ~ (-1), doubling for the first time for 30 days. Intraocular pressure (IOP) was measured and recorded by Schiotz intraocular pressure meter. At 30 days a week, all right eyes in group C underwent optical coherence tomography (OCT),) flash pan-field electroretinogram (Flash electroretinogram, FERG) and flash visual evoked potential (Flash visual evoked potential,). FVEP), optical coherence tomography (OCT) was used to detect the thickness of optic nerve fiber layer, and the amplitude of a wave and b wave were observed and compared by FERG. The degree of delay of N _ 1 P _ 1N _ 2 peak was observed by FVEP. Finally, the pathological changes of various layers of retina were observed with OLYMPUS optical microscope under 10 脳 40 times microscope. The results were statistically analyzed by SPSS13.0 statistical analysis software. Results: intraocular pressure (IOP): the intraocular pressure of group B C was higher than 22 mm Hg, and the highest was 55 mm Hg during the whole experiment. Statistical analysis of the thickness of optic nerve fiber layer measured by OCT showed that the thickness of optic nerve fiber layer was the thinnest in group B, followed by group C, and thickest in group A. The difference was statistically significant (P0.05). The statistical analysis of FERG results showed that the amplitude of a wave in group C was not significantly lower than that in group A, and there was no significant difference among the three groups (P0.05). The amplitude of B wave in group B was significantly lower than that in group A (P0.05), and that in group B was significantly lower than that in group C (P0.05). Statistical analysis of the results of each wave detected by FVEP showed that the peak time of three waves in group C was delayed than that in group A, and the delay in group B was significantly higher than that in group C (P0.05). Under light microscope, the structure of each layer of retina in group B was the most disordered, and that in group A was the most complete. Compared with group A, the structure of each layer of retina in group C changed slightly, but it was slightly different from that in group B. Conclusion: minocycline has protective effect on retinal injury induced by chronic intraocular pressure in rabbits.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R775.1
【参考文献】
相关期刊论文 前10条
1 王昌鹏,杨新光,王晓娟,李养军;兔慢性高眼压模型的建立[J];第四军医大学学报;2004年07期
2 石一宁,常宝琴,郭建强,伊恩晖,陈建华;视觉电生理和视野检查对原发性开角型青光眼早期诊断的敏感性[J];国际眼科杂志;2004年01期
3 马建洲;贺翔鸽;谢琳;许建涛;孙亚丽;王永堂;龙在云;李应玉;;慢性高眼压青光眼动物模型的构建和鉴定[J];国际眼科杂志;2007年04期
4 王艳;青光眼视神经保护治疗的实验研究[J];眼科新进展;2002年03期
5 王秀青,贺翔鸽,白继;甲基纤维素诱导兔慢性高眼压的实验研究[J];眼科新进展;2003年03期
6 徐亮;;原发性开角型青光眼早期诊断的难点及其对策(Ⅰ)[J];眼科;1996年01期
7 徐亮;慢性开角型青光眼早期诊断的难点及其对策(Ⅱ)[J];眼科;1997年03期
8 熊海波;刘苏;沈洁;晏兴云;;米诺环素对大鼠视网膜缺血再灌注的保护作用探讨[J];眼科研究;2008年06期
9 孙河,张慧,黄春娟,高祥春,王进;建立兔慢性高眼压模型方法的研究[J];中国中医眼科杂志;2005年04期
10 杨新光;于敬妮;王百忍;;兔慢性高眼压球后视神经损害的超微结构改变[J];中华眼底病杂志;2006年06期
，本文编号：2330388
本文链接：https://www.wllwen.com/yixuelunwen/yank/2330388.html
最近更新
教材专著
