凋亡抑制因子XIAP、Livin在喉癌中的表达和意义

发布时间：2018-11-21 19:10

【摘要】： 背景:喉癌(laryngeal carcinoma、cancer of the larynx、laryngeal cancer、Larynx cancer、carcinoma of larynx)是头颈部常见的恶性肿瘤,好发年龄为40～60岁,男性较女性多见,本癌的发生与吸烟、酗酒、胃食管反流、营养不良、乳头状瘤病毒感染、种族、性别、年龄、免疫系统功能减退、长期吸入有害物质及发声过度等因素有关。多年来,在喉全切除后行放射治疗是喉癌的标准治疗。患者全喉切除治疗后,发声功能完全丧失,也可能发生吞咽功能障碍,导致在许多方面生活质量下降,包括营养,社会功能和个人卫生。此外,放化疗综合治疗和单纯放疗组有比全喉切除术组在生存率控制的胜算低。所以,我们迫切需要找到治疗喉癌的新途径。目的:探讨凋亡抑制蛋白XIAP(X-染色体连锁蛋白)和Livin在喉癌组织中的表达及与病人的性别、年龄、临床分期、淋巴结转移和分化程度的相关性。方法:从107例2007年1月-2009年12月收治所有确诊为喉癌患者中,抽取42例喉癌及癌旁组织切片作对照,术前均未进行放疗及化疗。应用免疫组织化学二步法测定其XIAP、Livin蛋白的表达情况。结果: 1、XIAP在42例喉癌患者中阳性表达率为100%,在癌旁正常组织中阳性表达率为61.9%,两组间比较差异有统计学意义(P0.01);Livin在42例喉癌患者中阳性表达率为42.86%,在癌旁正常组织中阳性表达率为9.52%,两组间比较差异有统计学意义(P0.01) 2、在42例喉癌中,XIAP和Livin的阳性表达率在临床分期两组间比较差异有统计学意义(P0.01); 3、在42例喉癌患者中,XIAP和Livin的阳性表达率在性别、年龄、有无颈部淋巴结转移和分化程度各两组间比较,差异无统计学意义(P0.05)。结论:凋亡抑制蛋白XIAP和Livin在喉癌的发生发展中起重要作用,但和性别、年龄、有无颈部淋巴结转移和分化程度差异无统计学意义。
[Abstract]:Background: laryngeal carcinoma (laryngeal carcinoma,cancer of the larynx,laryngeal cancer,Larynx cancer,carcinoma of larynx) is a common malignant tumor in the head and neck. It is more common in men than in women. The incidence of this cancer is associated with smoking, alcoholism, gastroesophageal reflux and malnutrition. Papillomavirus infection, race, sex, age, hypofunction of immune system, long-term inhalation of harmful substances and hypervocal factors. Radiotherapy after total laryngectomy has been the standard treatment for laryngeal cancer for many years. After total laryngectomy, the vocal function is completely lost and the dysphagia may occur, leading to a decline in the quality of life in many areas, including nutrition, social function and personal hygiene. In addition, radiotherapy and chemotherapy combined therapy and radiotherapy alone have lower survival control than total laryngectomy. Therefore, we urgently need to find a new way to treat laryngeal cancer. Objective: to investigate the expression of XIAP and Livin in laryngeal carcinoma and their correlation with sex, age, clinical stage, lymph node metastasis and differentiation. Methods: from January 2007 to December 2009, 42 cases of laryngeal carcinoma and paracancerous tissue sections were selected from 107 patients with laryngeal cancer. No radiotherapy and chemotherapy were performed before operation. The expression of XIAP,Livin protein was detected by immunohistochemical two-step method. Results: 1the positive expression rate of XIAP was 100 in 42 patients with laryngeal carcinoma and 61.9 in the adjacent normal tissues. The difference between the two groups was statistically significant (P0.01). The positive expression rate of Livin was 42.86 in 42 cases of laryngeal carcinoma and 9.52 in the adjacent normal tissue. The difference between the two groups was statistically significant (P0.01) 2, and in 42 cases of laryngeal carcinoma. The positive expression rate of XIAP and Livin was significantly different between the two groups in clinical stage (P0.01). 3. In 42 patients with laryngeal carcinoma, the positive expression rates of XIAP and Livin in sex, age, cervical lymph node metastasis and differentiation were not significantly different between the two groups (P0.05). Conclusion: XIAP and Livin play an important role in the occurrence and development of laryngeal carcinoma, but there is no significant difference in sex, age, cervical lymph node metastasis and differentiation.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.65

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