湿性年龄相关性黄斑变性患者外周血单个核细胞Toll样受体3的表达及意义

发布时间：2018-11-22 06:43

【摘要】：目的 :探索Toll样受体(toll-like receptors,TLRs)3/4在湿性年龄相关性黄斑变性(age-related macular degeneration,AMD)患者外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)的表达及其意义。方法:25例湿性AMD患者和24例正常对照纳入本研究。患者与正常对照组均不患有糖尿病、冠心病、高血压等慢性疾病或免疫性疾病及与免疫相关的疾病。采用real-time PCR的方法检测TLR3/4的基因水平的表达,流式细胞术检测TLR3蛋白水平的表达。TLR3特定的配体PolyI∶C刺激后,ELISA检测白介素(interleukin,IL)-6、IL-8、单核细胞趋化蛋白1(monocyte chemotactic protein 1,MCP-1)的表达。结果:在PBMCs水平,AMD组TLR3在mRNA及蛋白水平表达均显著高于正常对照组,而TLR4的mRNA水平表达在2组之间无统计学差异。与对照组PBMCs相比,AMD组PBMCs IL-6及IL-8的表达明显升高,TLR3的配体PolyI∶C刺激后,AMD组PBMCs IL-6的表达显著增多,MCP-1、IL-8的表达在2组之间无统计学差异。结论:TLR3在AMD组表达上调,活化后可促进IL-6表达增多。由此推测TLR3信号通路可能通过促进促炎性细胞因子的表达在湿性AMD的发病发展中发挥着重要作用。
[Abstract]:Aim: to investigate the expression and significance of Toll like receptor (toll-like receptors,TLRs) 3 / 4 in peripheral blood mononuclear cells (peripheral blood mononuclear cells,PBMCs) in patients with wet age-related macular degeneration (age-related macular degeneration,AMD). Methods: 25 cases of wet AMD and 24 cases of normal control were included in this study. No diabetes, coronary heart disease, hypertension or immune disease or immune-related diseases were found in the patients and normal controls. The expression of TLR3/4 gene was detected by real-time PCR, and the expression of TLR3 protein was detected by flow cytometry. After the specific ligand PolyI:C was stimulated by TLR3, ELISA was used to detect the expression of interleukin-6 (interleukin,IL) IL-8. The expression of monocyte chemoattractant protein 1 (monocyte chemotactic protein 1 (MCP-1). Results: at the level of PBMCs, the expression of TLR3 and protein in AMD group was significantly higher than that in normal control group, but there was no significant difference in mRNA expression of TLR4 between the two groups. Compared with the control group, the expression of PBMCs IL-6 and IL-8 in the AMD group was significantly higher than that in the control group. The expression of PBMCs IL-6 in the AMD group was significantly increased after the stimulation of TLR3 ligand PolyI:C. There was no significant difference in the expression of MCP-1,IL-8 between the two groups. Conclusion: the expression of TLR3 was up-regulated in AMD group and increased after activation. It is suggested that TLR3 signaling pathway may play an important role in the pathogenesis and development of wet AMD by promoting the expression of pro-inflammatory cytokines.
【作者单位】：重庆医科大学附属第一医院眼科、重庆市眼科学重点实验室;
【基金】：重庆市卫生局重点资助项目(编号:2012-1-026)
【分类号】：R774.5

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