白酒对家兔眼压的影响

发布时间：2018-12-10 13:17

【摘要】：目的探讨家兔使用白酒灌胃后对眼压的影响,并比较不同剂量白酒对眼压的影响有无差异性,以便为研究人类饮酒后对眼压的影响提供参考。材料与方法健康成年家兔48只,完全随机分为A、B、C、D四组,其中A、B、C为实验组,D为对照组。以酒精度为56%(v/v)的白酒,分别按照不同的剂量对实验组灌胃:A组,2.5ml/kg;B组,5ml/kg;C组,7.5ml/kg,而对照组则灌以相应剂量的蒸馏水,并分别命名为Da、Db和Dc。采用Tono-Pen XL眼压计进行眼压测量。实验前测定固定时间点的多次眼压值作为基线值。灌胃实验开始前,先测量实验组与对照组的眼压值,并以该时刻作为初始时间点(0时刻)。立即对各实验组和对照组按相应的剂量以白酒和蒸馏水灌胃,灌胃后分别在0.5h、1.0h、1.5h、2.0h、2.5h、3.5h各时间点测量眼压。应用SPSS17软件包对实验前后所测眼压数据以及基线值数据进行统计分析和比较,分析白酒对家兔眼压的影响以及灌胃剂量的不同所产生的差异。结果利用SPSS17软件包进行单因素重复测量方差分析。A、B、C、D四组家兔间眼压的基线值相比:F=0.483;P0.05,无统计学差异。对照组Da、Db、Dc与D组的基线值相比:F=0.03;P0.05,无统计学差异。实验组中A组与对照组Da相比:F=0.255;P0.05,无统计学差异。实验组中B组与对照组Db相比:F=2.910;P0.05,且在0~1.0h之间家兔的眼压呈下降趋势,在1.0h处有明显差异,1.0~2.5h处开始明显回升。实验组中C组与对照组Dc相比:F=5.771;P0.05,且在0~1.5h之间呈下降趋势,在1.0h、1.5h、2.0h处有统计学差异。结论以剂量为5.0ml/kg(中剂量组)和7.5ml/kg(高剂量组)的白酒对家兔灌胃后,眼压在一定时间内有明显下降趋势。随着时间的延长,眼压逐步回升,剂量越大,降压幅度越大且较低眼压维持时间越长。而以2.5ml/kg(低剂量组)的白酒对家兔灌胃后,无明显的降压作用。同时由Da、Db、Db的比较结果表明,本实验中影响眼压值的因素为白酒,与蒸馏水(溶剂)无相关性。
[Abstract]:Objective to study the effect of different doses of liquor on intraocular pressure (IOP) in rabbits, and to provide a reference for the study of the effect of drinking liquor on IOP in rabbits. Materials and methods Forty-eight healthy adult rabbits were randomly divided into four groups: Acarb C as experimental group and D as control group. In the experiment group, 56% (v / v) liquor was given to the experimental group according to different doses: group A, group B, 2.5 ml / kg, 5 ml / kg; Group C, 7.5 ml / kg, while the control group was given distilled water of the corresponding dose, named Da,Db and Dc., respectively. The intraocular pressure was measured by Tono-Pen XL IOP meter. The intraocular pressure (IOP) at a fixed time point was measured as the baseline value before the experiment. The intraocular pressure (IOP) of the experimental group and the control group was measured before the gavage experiment, and the time was taken as the initial time point (time 0). The rats in each experimental group and control group were immediately treated with distilled water and liquor at the corresponding dosage. Intraocular pressure was measured at 0.5 h, 1.0 h, 1.5 h, 2.0 h, 2.5 h and 3.5 h, respectively. The intraocular pressure data and baseline data before and after the experiment were statistically analyzed and compared by SPSS17 software package. The effects of liquor on intraocular pressure in rabbits and the difference between the dosages of intragastric perfusion were analyzed. Results single factor repeated analysis of variance was carried out by using SPSS17 software package. The baseline values of IOP in the four groups were compared with each other: FG 0.483U P0.05. there was no statistical difference between the four groups. The baseline value of Da,Db,Dc in control group was not significantly different from that in group D (P 0.05). There was no significant difference in Da between group A and control group (P 0.05). Compared with the control group, the intraocular pressure (IOP) of group B was lower than that of the control group (P 0.05), and the IOP of group B decreased between 0 h and 1.0 h, and the intraocular pressure increased significantly at 1.0 h and 2.5 h, respectively. Compared with the control group, the Dc of group C was 5.771g / ml (P0.05), and it showed a decreasing trend between 0 and 1.5h, and there was a statistical difference at 1.0hs, 1.5h and 2.0h. Conclusion the intraocular pressure (IOP) of 5.0ml/kg (middle dose group) and 7.5ml/kg (high dose group) were significantly decreased after intragastric perfusion in rabbits. With the prolongation of time, intraocular pressure gradually increased, the larger the dose, the greater the amplitude of lowering blood pressure and the longer the duration of lower IOP. However, 2.5ml/kg (low dose group) liquor had no obvious antihypertensive effect on rabbits. At the same time, the results of Da,Db,Db showed that the factor affecting IOP in this experiment was liquor, which had no correlation with distilled water (solvent).
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R77

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