地塞米松对实验性过敏性鼻炎小鼠血清和鼻中隔黏膜TGF-β表达的影响
发布时间：2018-12-12 01:55
【摘要】： 目的：探讨地塞米松(Dexamethasone,DEX)对实验性过敏性鼻炎小鼠模型血清和鼻中隔黏膜中转化生长因子-β(transforming growth factor-β,TGF-β)表达的影响。方法：4-6周BALB/c小鼠40只,随机分为四组：正常对照A组、模型对照B组、治疗C组及治疗D组。以卵清蛋白(ovalbumin,OVA)作为致敏原；致敏实验小鼠、建立过敏性鼻炎小鼠模型。分析比较小鼠过敏症状评分,采用苏木素-伊红(hematoxylin-eosin,HE)染色法分析比较实验小鼠鼻中隔黏膜嗜酸性粒细胞(eosinophils,EOS)浸润程度。应用免疫组化法分析实验小鼠鼻中隔黏膜上皮细胞及炎性细胞内TGF-β表达情况。采用酶联免疫吸附法(ELISA)检测并分析比较实验小鼠外周血清中TGF-β及IgE的水平。应用spss11.5软件对实验数据进行统计学分析：实验数据均以x±s表示,采用单因素方差分析,组间比较采用t检验。检验水准取a=0.05,以P0.05为差异有统计学意义。 结果：B组小鼠鼻中隔黏膜组织嗜酸性粒细胞浸润显著高于A组(P0.01)；C组小鼠过敏症状评分、鼻中隔黏膜嗜酸性粒细胞浸润、血清IgE水平较B组显著降低(P0.01),其血清TGF-β水平较B组显著增高(P0.01)；D组小鼠仅鼻中隔黏膜嗜酸性粒细胞浸润低于B组(P0.01)；其过敏症状评分、血清IgE水平及血清TGF-β水平与B组比较无显著差异(P0.05)。A组小鼠鼻中隔黏膜未见TGF-β表达,B组小鼠鼻中隔黏膜内TGF-β表达明显,两个治疗组小鼠鼻中隔黏膜TGF-β表达均低于B组(P0.01)。 结论：地塞米松根据给药时间不同可分别抑制不同时相的鼻变态反应。基础致敏前使用地塞米松可抑制速发相和迟发相过敏反应；局部激发前使用地塞米松仅能抑制迟发相过敏反应。地塞米松可能至少通过两种机制影响TGF-β的表达,从而在过敏性鼻炎的治疗过程中发挥作用。一是通过上调外周血清TGF-β的水平从而抑制过敏反应的发生；二是通过降低鼻黏膜上皮细胞及嗜酸性粒细胞TGF-β的表达,抑制炎症细胞的增殖和趋化,从而抑制鼻部过敏性炎性反应的发生,最终可有效遏制鼻黏膜发生组织重塑。
[Abstract]:Aim: to investigate the effect of dexamethasone (Dexamethasone,DEX) on the expression of transforming growth factor 尾 (transforming growth factor- 尾 (TGF- 尾) in serum and septum mucosa of experimental allergic rhinitis mice. Methods: forty BALB/c mice at 4-6 weeks were randomly divided into four groups: normal control group A, model control group B, treatment group C and treatment group D. The mouse model of allergic rhinitis was established by using ovalbumin (ovalbumin,OVA) as allergen and sensitizing mice. The allergic symptom score of mice was analyzed and the infiltration of eosinophil (eosinophils,EOS) in nasal septum of experimental mice was analyzed by hematoxylin eosinophil (hematoxylin-eosin,HE) staining. The expression of TGF- 尾 in nasal septum mucosal epithelial cells and inflammatory cells was analyzed by immunohistochemical method. Enzyme linked immunosorbent assay (ELISA) was used to detect and compare the levels of TGF- 尾 and IgE in peripheral serum of experimental mice. Spss11.5 software was used to analyze the experimental data. The experimental data were expressed as x 卤s, single factor analysis of variance and t-test were used in the comparison between groups. The test level was 0. 05, with P 0. 05 as the difference. Results: the infiltration of eosinophils in nasal septum mucosa in group B was significantly higher than that in group A (P0.01). The allergic symptom score, eosinophil infiltration in nasal septum mucosa, serum IgE level and serum TGF- 尾 level in group C were significantly lower than those in group B (P0.01). The eosinophil infiltration in the nasal septum of group D was lower than that in group B (P0.01). There was no significant difference in allergy symptom score, serum IgE level and serum TGF- 尾 level between group B and group B (P0.05). No expression of TGF- 尾 was found in nasal septum mucosa of). A group, but TGF- 尾 expression in nasal septum mucosa of group B mice was significant. The expression of TGF- 尾 in nasal septum mucosa of both groups was lower than that in group B (P0.01). Conclusion: dexamethasone can inhibit nasal hypersensitivity in different phases according to the time of administration. The use of dexamethasone before basic sensitization can inhibit the rapid phase and delayed phase allergic reaction, and the use of dexamethasone before local excitation can only inhibit the delayed phase allergic reaction. Dexamethasone may play a role in the treatment of allergic rhinitis by affecting the expression of TGF- 尾 through at least two mechanisms. One is to inhibit the occurrence of allergic reaction by upregulating the level of TGF- 尾 in peripheral blood. Second, by reducing the expression of TGF- 尾 in nasal epithelial cells and eosinophils, inhibiting the proliferation and chemotaxis of inflammatory cells, thus inhibiting the occurrence of allergic inflammatory reaction in nasal mucosa, which can effectively curb the tissue remodeling of nasal mucosa.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R765.21
本文编号：2373669
[Abstract]:Aim: to investigate the effect of dexamethasone (Dexamethasone,DEX) on the expression of transforming growth factor 尾 (transforming growth factor- 尾 (TGF- 尾) in serum and septum mucosa of experimental allergic rhinitis mice. Methods: forty BALB/c mice at 4-6 weeks were randomly divided into four groups: normal control group A, model control group B, treatment group C and treatment group D. The mouse model of allergic rhinitis was established by using ovalbumin (ovalbumin,OVA) as allergen and sensitizing mice. The allergic symptom score of mice was analyzed and the infiltration of eosinophil (eosinophils,EOS) in nasal septum of experimental mice was analyzed by hematoxylin eosinophil (hematoxylin-eosin,HE) staining. The expression of TGF- 尾 in nasal septum mucosal epithelial cells and inflammatory cells was analyzed by immunohistochemical method. Enzyme linked immunosorbent assay (ELISA) was used to detect and compare the levels of TGF- 尾 and IgE in peripheral serum of experimental mice. Spss11.5 software was used to analyze the experimental data. The experimental data were expressed as x 卤s, single factor analysis of variance and t-test were used in the comparison between groups. The test level was 0. 05, with P 0. 05 as the difference. Results: the infiltration of eosinophils in nasal septum mucosa in group B was significantly higher than that in group A (P0.01). The allergic symptom score, eosinophil infiltration in nasal septum mucosa, serum IgE level and serum TGF- 尾 level in group C were significantly lower than those in group B (P0.01). The eosinophil infiltration in the nasal septum of group D was lower than that in group B (P0.01). There was no significant difference in allergy symptom score, serum IgE level and serum TGF- 尾 level between group B and group B (P0.05). No expression of TGF- 尾 was found in nasal septum mucosa of). A group, but TGF- 尾 expression in nasal septum mucosa of group B mice was significant. The expression of TGF- 尾 in nasal septum mucosa of both groups was lower than that in group B (P0.01). Conclusion: dexamethasone can inhibit nasal hypersensitivity in different phases according to the time of administration. The use of dexamethasone before basic sensitization can inhibit the rapid phase and delayed phase allergic reaction, and the use of dexamethasone before local excitation can only inhibit the delayed phase allergic reaction. Dexamethasone may play a role in the treatment of allergic rhinitis by affecting the expression of TGF- 尾 through at least two mechanisms. One is to inhibit the occurrence of allergic reaction by upregulating the level of TGF- 尾 in peripheral blood. Second, by reducing the expression of TGF- 尾 in nasal epithelial cells and eosinophils, inhibiting the proliferation and chemotaxis of inflammatory cells, thus inhibiting the occurrence of allergic inflammatory reaction in nasal mucosa, which can effectively curb the tissue remodeling of nasal mucosa.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R765.21
【引证文献】
相关期刊论文 前1条
1 胡艳菲;唐方;;过敏性鼻炎动物模型研究进展[J];医学研究生学报;2012年05期
，本文编号：2373669
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