经眼动脉灌注治疗兔成视网膜细胞瘤

发布时间：2018-12-27 10:43

【摘要】：目的评估兔成视网膜细胞瘤(retinoblastoma,RB)模型的建立及经导管眼动脉灌注(selective ophthalmic arterial injection,SOAI)治疗兔RB的可行性。方法 HXO-Rb44RB细胞予中心细胞室培养,取对数生长期HXO-Rb44细胞,用PBS配成浓度为2.0×107个/ml的细胞悬液,抽取0.1 ml细胞悬液注射于6只SPF级新西兰兔右眼视网膜间隙下。接种肿瘤细胞前3 d和注射后1个月臀部注射环孢素A 15 mg·kg-1·d-1,1个月后减量至10 mg·kg-1·d-1并持续到实验结束。将RB兔分为对照组3只和实验组3只,对照组灌注0.9%氯化钠溶液15 ml,实验组将美法仑2.5 mg用0.9%氯化钠溶液稀释至15 ml后灌注。术后分别于7、14 d比较两组肿瘤生长情况。结果注射环孢素A期间,实验兔不良反应轻微,6只均建模成功。7次插管中6次成功插至眼动脉。SOAI术后1周,实验组肿瘤可见明显缩小,2周后均消失。SOAI术后,实验组仅1只见右眼轻度红肿、流泪,1周后红肿自行消退。结论 SOAI治疗RB兔具有可行性,为进一步经眼动脉灌注治疗RB的基础研究提供了参考。
[Abstract]:Objective to evaluate the establishment of rabbit retinoblastoma (retinoblastoma,RB) model and the feasibility of transcatheter ophthalmic artery infusion of (selective ophthalmic arterial injection,SOAI in the treatment of rabbit RB. Methods HXO-Rb44RB cells were cultured in the center cell chamber. The HXO-Rb44 cells in logarithmic growth phase were collected and injected into the right retinal space of 6 SPF grade New Zealand rabbits with PBS admixture of 2.0 脳 107 cells / ml. Cyclosporine A 15 mg kg-1 d -1 was injected into the buttocks 3 days before inoculation and 1 month after injection, and then decreased to 10 mg kg-1 d -1 one month later and continued until the end of the experiment. RB rabbits were divided into control group (n = 3) and experimental group (n = 3). The control group was perfused with 0.9% sodium chloride solution for 15 ml,. The tumor growth was compared between the two groups on the 7th and 14th day after operation. Results during the period of cyclosporine A injection, the adverse reaction of experimental rabbits was slight, and 6 rabbits were successfully modeled, 6 out of 7 intubation were successfully inserted into the ophthalmic artery. 1 week after SOAI, the tumor in the experimental group was obviously reduced and disappeared 2 weeks later. SOAI operation was performed. In the experimental group, the right eye was mild redness and tears, and the swelling subsided 1 week later. Conclusion SOAI is feasible for the treatment of RB rabbits, which provides a reference for the further study of transocular arterial infusion of RB.
【作者单位】：第二军医大学附属长征医院放射科;广州市妇女儿童医疗中心介入与血管瘤科;
【基金】：广州市科技和信息化局项目(2012J4100032)
【分类号】：R739.72

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