顺铂激活的低分化鼻咽癌细胞氯电流为非钙激活氯电流

发布时间：2018-12-30 13:18

【摘要】：目的:探讨顺铂激活的低分化鼻咽癌细胞(CNE-2Z)氯通道电流是否为钙激活的氯电流。方法:采用膜片钳全细胞记录技术记录细胞内/外无Ca2+及钙通道阻断剂对顺铂激活氯电流的影响,并用高渗灌流液观察顺铂激活氯电流的容积敏感性。结果:去除细胞外液的Ca2+后,5μmol/L顺铂能诱发氯电流,且电流大小与细胞外有Ca2+时无明显差异,但潜伏期与达峰时间延长。细胞内外均无Ca2+对顺铂激活氯电流未产生影响。钙通道阻断剂nifedipine未能抑制顺铂诱发的氯电流。但细胞外灌流高渗液几乎可完全抑制顺铂激活的氯电流。结论:顺铂激活的氯通道开放不依赖于细胞内/外的Ca2+,该通道不是钙激活氯通道而很可能是容积敏感性氯通道。
[Abstract]:Aim: to investigate whether the chloride channel current in poorly differentiated nasopharyngeal carcinoma (CNE-2Z) cells activated by cisplatin is calcium activated chloride current. Methods: the effects of intracellular / extracellular Ca2 and calcium channel blocker on the chloride current activated by cisplatin were recorded by patch clamp whole-cell recording technique. The volumetric sensitivity of cisplatin activated chloride current was observed by high percolation fluid. Results: after the removal of Ca2 from extracellular fluid, 5 渭 mol/L cisplatin could induce chloride current, and the current size was not significantly different from that of extracellular Ca2, but the latency and peak time were prolonged. There was no effect of Ca2 on the chloride current activated by cisplatin. Nifedipine, a calcium channel blocker, failed to inhibit the chloride current induced by cisplatin. However, extracellular perfusion with high percolation almost completely inhibited the chloride current activated by cisplatin. Conclusion: the opening of Cisplatin-activated chloride channel is independent of intracellular / extracellular Ca2. It is not calcium-activated chloride channel but probably volume-sensitive chloride channel.
【作者单位】：广州医科大学卫生职业技术学院生理学教研室;暨南大学医学院药理学系;暨南大学医学院生理学系;广东医学院生理学教研室;广东药学院生物学教研室;
【基金】：国家自然科学基金资助项目(No.81173064;No.81272223;No.81273539) 教育部博士点基金资助项目(No.20124401110009) 广东省医学科学技术研究基金资助项目(No.B2012187) 广州市科技计划项目(No.2013J500015) 东莞市科技计划项目(No.2011108102006)
【分类号】：R739.63

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