HRD1在糖尿病小鼠视网膜的表达变化

发布时间：2019-01-08 20:49

【摘要】：目的观察羟甲基戊二酰辅酶A还原酶降解蛋白1(Hmg Co A reductase degradation 1,HRD1)在糖尿病小鼠视网膜不同时期的表达变化。方法 STZ诱导的糖尿病C57BL/J小鼠,分别于糖尿病成功造模后1个月、3个月、6个月,选取糖尿病小鼠视网膜组织,利用免疫荧光、Western blot、RT-PCR技术检测HRD1在糖尿病不同时期的表达变化。结果免疫荧光观察到HRD1绿色荧光强度在1个月、3个月、6个月糖尿病小鼠视网膜随糖尿病进程逐渐减弱。Western blot、RT-PCR检测可见在1个月、3个月、6个月糖尿病小鼠视网膜组织HRD1基因水平和蛋白水平表达较对照组均明显减少(m RNA:P1=0.040 5;P2=0.006 2;P3=0.004 1;蛋白:P1=0.010 9;P2=0.001 3;P3=0.000 3)。结论HRD1可能在糖尿病视网膜病变发生发展过程中起到一定作用。
[Abstract]:Objective to observe the expression of hydroxymethyl glutaryl coA reductase degrading protein 1 (Hmg Co A reductase degradation 1 (HRD1) in the retina of diabetic mice at different stages. Methods Diabetic C57BL/J mice induced by STZ were selected from retina of diabetic mice at 1 month, 3 months and 6 months after successful modeling of diabetes mellitus. Immunofluorescence, Western blot, was used. RT-PCR technique was used to detect the expression of HRD1 at different stages of diabetes mellitus. Results the intensity of green fluorescence of HRD1 decreased gradually with the progression of diabetes in 1 month, 3 months and 6 months of diabetic mice, and Western blot,RT-PCR detection was observed at 1 month, 3 months and 6 months, respectively. The expression of HRD1 gene and protein in the retina of 6 months diabetic mice was significantly lower than that in the control group (m RNA:P1=0.040 5). P _ (2) O _ (0.006 2) P _ (3) O _ (0.0041); protein: P _ (1) 0.010 ~ (9) P ~ (2 +) ~ (0.001) P _ (3) 0.000 ~ (3). Conclusion HRD1 may play a role in the development of diabetic retinopathy.
【作者单位】：华中科技大学同济医学院附属同济医院眼屈光治疗中心;华中科技大学同济医学院附属同济医院眼科;
【基金】：国家自然科学基金资助(编号:81271015) 湖北省自然科学基金资助(编号:2009CDB206)~~
【分类号】：R587.2;R774.1

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