喉鳞癌及癌旁正常组织miRNA差异表达分析

发布时间：2019-01-27 17:14

【摘要】：目的:应用基因芯片技术,研究喉鳞状细胞癌中miRNA的表达谱,筛选喉鳞癌和癌旁正常中组织差异表达的miRNA,寻找可能的分子生物学指标。方法:选取2008-06-01-2009-04-30中国医学科学院肿瘤医院8例喉鳞癌及其配对的癌旁正常组织(距肿瘤组织1cm),Trizol法提取总RNA,经Cy3荧光标记后与含有人723条miRNA探针的芯片进行杂交,筛选在喉鳞癌和癌旁正常组织中差异表达的miRNA。结果:喉鳞癌及癌旁组织中提取的总RNA均符合芯片杂交要求,RNA完整性评分1.8~2.1;共有132个miRNA在8例喉鳞癌患者中均有表达。喉鳞癌和癌旁正常组织中差异表达的miRNA有53个,其中22个出现上调,31个出现下调,差异表达≥5倍的miRNA共有8个,上调的有miR-196b、miR-196a、miR-7和miR-183,下调的有miR-375、miR-139-5p、miR-338-3p和miR-370,其中miR-196b、miR-196a和miR-375差异表达≥8倍。采用TargetScan、Pictar、miRanda和MirTarget2等4种算法数据库预测这8种差异表达的miRNA的靶基因,4种数据库中均预测到的靶基因共183个。结论:通过基因芯片的方法,筛选到喉鳞癌和癌旁组织中差异表达的miRNA,对其中明显差异表达的miRNA及其靶基因的深入研究有可能找到新的喉鳞癌分子肿瘤学标志,为进一步研究喉鳞癌发病机制打下基础。
[Abstract]:Objective: to study the expression of miRNA in laryngeal squamous cell carcinoma (LSCC) by using gene chip technique, and to screen the differentially expressed miRNA, in laryngeal squamous cell carcinoma (LSCC) and adjacent normal tissues to find possible molecular biological markers. Methods: eight patients with laryngeal squamous cell carcinoma (LSCC) and their matched adjacent normal tissues were selected from Cancer Hospital of the Chinese Academy of Medical Sciences from January 2008-01-2009-04-30. Total RNA, was extracted by 1cm), Trizol method from tumor tissues. Cy3 labeled microarray containing 723 human miRNA probes was used to screen differentially expressed miRNA. in laryngeal squamous cell carcinoma and adjacent normal tissues. Results: the total RNA extracted from laryngeal squamous cell carcinoma and adjacent tissues met the requirement of chip hybridization, and the RNA integrity score was 1.8m2.1.A total of 132 miRNA were expressed in 8 cases of laryngeal squamous cell carcinoma. There were 53 miRNA differentially expressed in laryngeal squamous cell carcinoma and adjacent normal tissues, of which 22 were up-regulated and 31 down-regulated. There were 8 miRNA whose differential expression was more than 5 times, and miR-196b,miR-196a,miR-7 and miR-183, were up-regulated. The expression of miR-196b,miR-196a and miR-375 was more than 8 times lower than that of miR-375,miR-139-5p,miR-338-3p and miR-370,. The target genes of the eight differentially expressed miRNA were predicted by TargetScan,Pictar,miRanda and MirTarget2, and 183 target genes were predicted in each of the four databases. Conclusion: the differential expression of miRNA, in laryngeal squamous cell carcinoma and paracancerous tissues was screened by the method of gene microarray. The further study of miRNA and its target gene in laryngeal squamous cell carcinoma may find a new molecular oncology marker of laryngeal squamous cell carcinoma. To lay a foundation for further study of the pathogenesis of laryngeal squamous cell carcinoma.
【作者单位】：深圳市第二人民医院;深圳大学第一附院耳鼻喉科;中国医学科学院肿瘤医院头颈外科;中国医学科学院肿瘤研究所病因与癌变研究室;
【分类号】：R739.65

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