Galectin-3、Bcl-2、MMP-2及K-ras在喉癌中的表达及临床意义

发布时间：2019-01-27 17:57

【摘要】： 目的: 研究癌基因galectin-3、bcl-2、mmp-2及k-ras在喉鳞状细胞癌中的表达、临床意义以及它们之间的相互关系。方法: 采用免疫组化SP法检测32例喉鳞状细胞癌(手术前均未进行放疗、化疗或生物治疗)组织、32例正常组织及10例声带息肉组织标本中galectin-3、bcl-2、mmp-2及k-ras的表达,并对四者的表达与喉癌病理分级、临床分期及淋巴结转移之间的关系进行统计学分析,同时对galectin-3与bcl-2、mmp-2及k-ras三者的相关性分别进行相关性分析。结果: galectin-3、bcl-2、mmp-2及k-ras阳性表达部位主要位于细胞质内。galectin-3、bcl-2、mmp-2及k-ras在32例喉正常组织中的阳性表达率分别是34.4%、0、9.4%、0,而在32例喉鳞癌中的阳性表达率分别是87.5%、62.5%%、75%、81.25%,galectin-3、bcl-2、mmp-2及k-ras 4项指标在喉癌中的表达均和其在正常组织中表达有显著的差异(p0.05);喉正常组织、声带息肉组织及喉癌组织三组间galectin-3、bcl-2、mmp-2及k-ras的表达差异有统计学意义;galectin-3和bcl-2的表达与喉癌病理分级相关(p0.05);bcl-2的表达与喉癌临床分期及淋巴结转移相关(p0.05);galectin-3与bcl-2、galectin-3与mmp-2及galectin-3与k-ras三组癌基因在喉癌中的表达呈正相关(r1=0.993 r2=0.989 r3=0.992)。结论: 1、galectin-3、mmp-2及k-ras的高表达与喉癌的发生密切相关。 2、bcl-2的表达增高与喉癌的发生、发展、恶性程度及淋巴结转移密切相关。 3、galectin-3与bcl-2、mmp-2及k-ras的协同作用促进了喉癌的发生、发展,对galectin-3、bcl-2、mmp-2及k-ras的检测可能有助于喉癌的早期诊断及预后判断,并为喉癌的基因治疗提供新的理论依据。galectin-3有望成为喉癌基因治疗的新的靶基因。
[Abstract]:Aim: to investigate the expression and clinical significance of oncogene galectin-3,bcl-2,mmp-2 and k-ras in laryngeal squamous cell carcinoma (LSCC). Methods: immunohistochemical SP method was used to detect galectin-3,bcl-2, in 32 cases of laryngeal squamous cell carcinoma (without radiotherapy, chemotherapy or biological therapy before operation), 32 normal tissues and 10 cases of vocal cord polyps. The expression of mmp-2 and k-ras, and the relationship between the expression of galectin-3 and bcl-2, and the pathological grade, clinical stage and lymph node metastasis of laryngeal carcinoma were analyzed statistically. At the same time, the relationship between galectin-3 and bcl-2, was also analyzed. The correlation between mmp-2 and k-ras was analyzed. Results: the positive expression sites of galectin-3,bcl-2,mmp-2 and k-ras were mainly located in cytoplasm. The positive expression rates of galectin-3,bcl-2,mmp-2 and k-ras in 32 normal laryngeal tissues were 34. 4%, respectively. The positive expression rates in 32 cases of laryngeal squamous cell carcinoma were 87.5 and 62.5, respectively. The expression of mmp-2 and k-ras in laryngeal carcinoma was significantly different from that in normal tissues (p0.05). The expression of galectin-3,bcl-2,mmp-2 and k-ras in normal laryngeal tissue, vocal cord polyp tissue and laryngeal carcinoma tissue were significantly different, and the expression of galectin-3 and bcl-2 were related to the pathological grade of laryngeal carcinoma (p0.05). The expression of bcl-2 was correlated with the clinical stage and lymph node metastasis of laryngeal carcinoma (p0.05). There was a positive correlation between galectin-3 and bcl-2,galectin-3 and mmp-2 and galectin-3 and k-ras in laryngeal carcinoma. Conclusion: 1 the high expression of galectin-3 mmp-2 and k-ras is closely related to the occurrence of laryngeal carcinoma. 2 the increased expression of bcl-2 is closely related to the occurrence, development, malignancy and lymph node metastasis of laryngeal carcinoma. 3 the synergistic effect of galectin-3 with bcl-2,mmp-2 and k-ras promotes the occurrence and development of laryngeal carcinoma. The detection of galectin-3,bcl-2,mmp-2 and k-ras may be helpful to the early diagnosis and prognosis of laryngeal carcinoma. Galectin-3 is expected to be a new target gene for laryngeal cancer gene therapy.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.65

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