糖尿病视网膜病变大鼠视网膜动脉平滑肌细胞中钙离子浓度的变化

发布时间：2019-02-14 16:28

【摘要】：目的探讨糖尿病视网膜病变SD大鼠视网膜动脉平滑肌细胞中钙离子浓度的变化。方法取40只正常雄性SD大鼠,采用链脲佐菌素(streptozotocin,STZ)100 mg·kg-1腹腔内注射建立糖尿病大鼠模型,持续6个月后经眼底荧光血管造影证实各大鼠出现糖尿病视网膜病变,作为糖尿病视网膜病变组。同时选择正常SD大鼠40只及单纯形成糖尿病的大鼠40只分别用作正常对照组及单纯糖尿病组。酶消化法分离出视网膜动脉平滑肌细胞,荧光测定仪测定三组大鼠视网膜动脉平滑肌细胞内钙离子浓度,SPSS17.0软件进行分析,以P0.05为差异有统计学意义。结果造模8周后,测得正常对照组和单纯糖尿病组大鼠血糖水平分别为(6.77±0.18)mmol·L-1和(31.14±0.75)mmol·L-1,符合成模条件,6个月后糖尿病视网膜病变组大鼠眼底荧光血管造影确定为糖尿病视网膜病变SD大鼠模型。糖尿病视网膜病变大鼠视网膜动脉平滑肌细胞内钙离子浓度(255±10)nmol·L-1较正常对照组(123±11)nmol·L-1和单纯糖尿病组(152±23)nmol·L-1明显增高,三组之间差异具有统计学意义(P0.05)。结论糖尿病视网膜病变视网膜动脉平滑肌细胞内钙离子浓度升高可能成为糖尿病视网膜动脉异常收缩及功能异常的重要原因。
[Abstract]:Objective To study the changes of calcium ion concentration in the retinal artery smooth muscle cells of SD rats with diabetic retinopathy. Methods 40 normal male SD rats were injected intraperitoneally with streptozoocin (STZ) 100 mg 路 kg-1, and the diabetic retinopathy was confirmed by fundus fluorescein angiography for 6 months, as a diabetic retinopathy group. At the same time, 40 normal SD rats and 40 diabetic rats were selected as the normal control group and the simple diabetes group. The concentration of calcium in the rat retinal artery smooth muscle cells was measured by the enzyme digestion method, and the concentration of calcium ion and SPSS17.0 in the rat retinal artery smooth muscle cells were measured by the fluorescence measurement instrument. Results The blood glucose levels in the normal control group and the pure DM group were (6.77% 0.18) mmol 路 L-1 and (314.00. 75) mmol 路 L-1, respectively. The model of SD rats with diabetic retinopathy was determined by fundus fluorescein angiography in diabetic retinopathy group after 6 months. The intracellular calcium ion concentration (255-10) nmol 路 L-1 in the retinal artery smooth muscle cells of diabetic retinopathy was significantly higher than that in the normal control group (123-11) nmol 路 L-1 and the simple-type diabetes group (152-23) nmol 路 L-1, and the difference between the three groups was statistically significant (P0.05). Conclusion The increase of calcium ion concentration in the retinal artery smooth muscle cells of diabetic retinopathy may be an important cause of the abnormal contraction and abnormal function of the diabetic retinopathy.
【作者单位】：南京医科大学附属无锡人民医院眼科;南京医科大学附属无锡人民医院心脏中心;
【基金】：江苏省自然科学基金(编号:SBK201222073) 医管中心联合攻关项目(编号:YGZX1206)~~
【分类号】：R587.2;R774.1

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