IGF-2与VEGF在翼状胬肉中的表达及意义

发布时间：2019-04-01 16:58

【摘要】：目的：本实验通过对胰岛素样生长因子2(IGF-2)与血管内皮生长因子(VEGF)在翼状胬肉中表达情况的研究,进一步探讨二者之间的相关性,为深入地研究和积极治疗翼状胬肉提供理论依据,并且为术前用药、术后防止复发奠定基础。方法：研究对象均来自2009年11月至2010年10月期间在延边大学附属医院手术治疗的翼状胬肉患者及斜视患者共52例。实验前均排除其他眼部疾病。实验组：选取胬肉患者翼状胬肉标本42例,患者年龄在38岁至65岁之间,均单眼发病(男性19例,女性23例)。按病变组织有无充血、肥厚将翼状胬肉分为两组：(1)静止期组17例,(2)进行期组25例。对照组：选择斜视患者正常结膜标本10例(均为球结膜),患者年龄在32岁至58岁之间(男性6例,女性4例)。采用免疫组化SABC法分别检测IGF-2与VEGF在两组之间的表达情况。结果： 1.IGF-2在翼状胬肉进行期组、翼状胬肉静止期组、正常结膜组的阳性表达率分别为80.0%、41.2%、0.0%,进行期组分别与静止期组、正常结膜组相比较,差异有统计学意义(P0.05);翼状胬肉静止期组与正常结膜组相比较,差异无统计学意义(P0.05)。 2. VEGF在翼状胬肉进行期组、翼状胬肉静止期组、正常结膜组的阳性表达率分别为92.0%、52.9%、30.0%,进行期组分别与静止期组、正常结膜组相比较,差异有统计学意义(P0.05);翼状胬肉静止期组与正常结膜组相比较,差异无统计学意义(P0.05)。 3.用等级资料的相关性分析检验在翼状胬肉中IGF-2与VEGF两种因子阳性表达的相关性,结果显示二项指标的表达结果相互间呈正相关(r=0.378,P0.05)。结论： 1.IGF-2与VEGF在翼状胬肉进行期、静止期中的表达均高于正常结膜； 2.IGF-2与VEGF可能促使翼状胬肉的发生、发展； 3.IGF-2与VEGF在翼状胬肉的发病机制中可能具有协同作用。
[Abstract]:Objective: to study the expression of insulin-like growth factor 2 (IGF-2) and vascular endothelial growth factor (VEGF) in pterygium. To provide theoretical basis for further study and active treatment of pterygium, and to lay a foundation for pre-operative medication and prevention of recurrence after operation. Methods: from November 2009 to October 2010, 52 patients with pterygium and strabismus were treated in the affiliated Hospital of Yanbian University. Other ocular diseases were excluded before the experiment. Experimental group: 42 pterygium patients aged between 38 and 65 years old were selected from pterygium patients (M 19, F 23). According to hyperemia or not, pterygium was divided into two groups: (1) static group (17 cases) and (2) stage group (25 cases). Control group: 10 normal conjunctiva specimens (all bulbar conjunctiva) were selected from patients with strabismus. The age of the patients ranged from 32 to 58 years old (M 6, F 4). Immunohistochemical SABC method was used to detect the expression of IGF-2 and VEGF between the two groups. Results: the positive expression rate of 1.IGF-2 in pterygium progression group, pterygium stationary stage group and normal conjunctiva group were 80.0%, 41.2% and 0.0%, respectively. The difference between normal conjunctiva group and normal conjunctiva group was statistically significant (P0.05). There was no significant difference between pterygium group and normal conjunctiva group (P0.05). 2. The positive expression rate of VEGF in pterygium progression group, pterygium stationary stage group and normal conjunctiva group were 92.0%, 52.9%, 30.0%, respectively. The difference was statistically significant (P0.05); There was no significant difference between pterygium group and normal conjunctiva group (P0.05). 3. The positive expression of IGF-2 and VEGF in pterygium was tested by the correlation analysis of grade data. The results showed that the two indexes were positively correlated with each other (r = 0.378, P0.05). Conclusion: the expression of 1.IGF-2 and VEGF in pterygium is higher than that in normal conjunctiva, 2.IGF-2 and VEGF may promote the occurrence and development of pterygium. 3.IGF-2 and VEGF may play a synergistic role in the pathogenesis of pterygium.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R779.6

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