体外扩增自体脂肪来源干细胞移植恢复鼻黏膜功能

发布时间：2019-04-27 09:13

【摘要】：背景:体外扩增的自体脂肪来源干细胞具有多向分化的潜能,在组织工程和再生医学领域具有广阔的应用前景。目的:观察自体脂肪来源干细胞移植受损人鼻黏膜组织的结构和功能变化。方法:选取因物理或化学因素造成鼻黏膜损害的患者10例,其中6例黏膜瘢痕,4例黏膜破溃,抽取自体脂肪组织进行脂肪来源干细胞体外分离、培养、扩增,移植前进行质量安全性检测。6例患者在黏膜瘢痕基底部、4例患者在黏膜破溃处注射移植脂肪来源干细胞(1×107/cm2),共注射3次,每2次间隔15 d。末次注射移植后30,90,150 d分别行鼻腔容积、最小横断面积、黏膜纤毛清除功能测定,其中3例患者在移植前和移植后30 d,取0.1 cm×0.1 cm黏膜组织,进行苏木精-伊红染色、Masson三色染色、AB-PAS染色。结果与结论:脂肪来源干细胞注射移植后,所有患者临床症状得到缓解。与移植前比较,脂肪来源干细胞注射移植后30,90,150 d鼻腔容积及鼻腔最小横断面积有所减少(P0.05),鼻黏膜纤毛清除功能有所改善,但差异无显著性意义(P0.05)。与移植前相比,脂肪来源干细胞移植30 d时3例患者鼻黏膜炎症明显减轻,胶原纤维排列整齐、沉积减少,杯状细胞分泌的黏蛋白增多。结果证明了体外扩增的脂肪来源干细胞可以重建鼻黏膜结构和恢复其功能。
[Abstract]:Background: autologous adipose-derived stem cells (ADSCs) expanded in vitro have the potential for multidirectional differentiation and have broad application prospects in tissue engineering and regenerative medicine. Objective: to observe the structural and functional changes of human nasal mucosa after autologous adipose-derived stem cell transplantation. Methods: 10 patients with nasal mucosal damage caused by physical or chemical factors were selected, including 6 cases of mucosal scar and 4 cases of mucosal rupture. Autologous adipose tissue was extracted and isolated, cultured, and amplified from adipose-derived stem cells in vitro. Before transplantation, 6 patients were injected with transplanted adipose-derived stem cells (1 脳 10 ~ 7 / cm2) at the base of cicatricial base of mucosa, and 4 patients were injected with transplanted adipose-derived stem cells (1 脳 10 ~ 7 / cm2) at the base of mucosal cicatricial base, for 15 d every 2 times. Nasal cavity volume, minimum cross-sectional area and mucociliary clearance function were measured 30 and 90150 days after the last injection. The mucosal tissue of 0.1 cm 脳 0.1 cm was stained with hematoxylin-eosin in 3 patients before transplantation and 30 days after transplantation. Masson trichrome staining, AB-PAS staining. Results & conclusion: after transplantation of adipose-derived stem cells, the clinical symptoms of all patients were relieved. Compared with pre-transplantation, the volume of nasal cavity and the minimum cross-sectional area of nasal cavity were decreased at 30 and 90150 days after adipose-derived stem cell transplantation (P0.05), and the ciliary clearance function of nasal mucosa was improved, but there was no significant difference (P0.05). At 30 days after adipose-derived stem cell transplantation, the inflammation of nasal mucosa in 3 patients was significantly alleviated, collagen fibers were arranged neatly, deposition decreased, and mucin secreted by goblet cells increased. The results showed that the adipose-derived stem cells expanded in vitro could reconstruct the nasal mucosa structure and restore its function.
【作者单位】：武装警察部队总医院皮肤再生医学科;
【分类号】：R765.9

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